| 质粒名称: | pXR002: EF1a-dCasRx-2A-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 109050 |
| 存储实验室: | Patrick Hsu |
|
载体骨架基本信息 |
|
| 载体名称: | lenti(AMP) |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 12990 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | GFP |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCasRx,NLS-dRfxCas13d-NLS |
| 物种来源: | H. sapiens (human); Ruminococcus flavefaciens XPD3002 |
| 插入大小: | 3000 bp |
| 基因突变: | R239A/H244A/R858A/H863A |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
NLS（插入件上的N端子）
NLS（插接件上的C端子）
HA（插入件上的C端子）
2A eGFP（主干上的C端）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXR002: EF1a-dCasRx-2A-EGFP was a gift from Patrick Hsu (Addgene plasmid # 109050 ; http://n2t.net/addgene:109050 ; RRID:Addgene_109050) |
| 参考文献部分: | Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. 10.1016/j.cell.2018.02.033 PubMed 29551272 |
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pXR002:EF1a-dCasRx-2A-EGFP载体质粒基本信息
pXR002:EF1a-dCasRx-2A-EGFP质粒图谱载体图谱和pXR002:EF1a-dCasRx-2A-EGFP载体序列质粒序列多克隆位点信息
pXR002:EF1a-dCasRx-2A-EGFP质粒载体简介
pXR002:EF1a-dCasRx-2A-EGFP质粒序列载体序列
LOCUS pXR002: EF1a-dCasRx-2A-EGFP 12991 bp ds-DNA circular SYN 20-JUN-2019
DEFINITION Catalytically inactive dCasRx with 2A-EGFP.
ACCESSION .
VERSION .
KEYWORDS pXR002: EF1a-dCasRx-2A-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12991)
AUTHORS Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD
TITLE Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR
Effectors.
JOURNAL Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi:
10.1016/j.cell.2018.02.033.
PUBMED 29551272
REFERENCE 2 (bases 1 to 12991)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 20, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12991
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(42..61)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 233..612
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 614..812
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 767..787
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 830..1010
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1057..1182
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1675..1908
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2435..2552
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2677..3855
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2908..3846
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3803..3823
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3874..3894
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6811..6831
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6841..6867
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 6883..6936
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6937..7653
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(6979..7000)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7240..7259)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7587..7608
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7678..8266
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7731..7751)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8149..8160)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8269..8285)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8270..8286)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8791..8971
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8997..9014)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9003..9227
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9273..9701
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9360..9379)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9570..9591
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9710..9730)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9715..10044
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9895..10030
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9957..9976
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10092..10139
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10158..10532
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10662..10783
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10699..10718)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10753..10772
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10832..10848)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10832..10848)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10845..10867)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10856..10872
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10880..10910)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10925..10946
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11063..11080)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11234..11822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11314..11333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11993..12853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12616..12635
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12854..12958)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
781 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
1021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
1501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
2581 cagtttggtt aattaatgca aagatggata aagttttaaa cagagaggaa tctttgcagc
2641 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
2701 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
2761 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
2821 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
2881 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
2941 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
3001 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
3061 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
3121 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
3181 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
3241 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
3301 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
3361 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
3421 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
3481 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
3541 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
3601 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
3661 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
3721 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
3781 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3841 tttcaggtgt cgtgacgtac ggccaccatg agccccaaga agaagagaaa ggtggaggcc
3901 agcatcgaaa aaaaaaagtc cttcgccaag ggcatgggcg tgaagtccac actcgtgtcc
3961 ggctccaaag tgtacatgac aaccttcgcc gaaggcagcg acgccaggct ggaaaagatc
4021 gtggagggcg acagcatcag gagcgtgaat gagggcgagg ccttcagcgc tgaaatggcc
4081 gataaaaacg ccggctataa gatcggcaac gccaaattca gccatcctaa gggctacgcc
4141 gtggtggcta acaaccctct gtatacagga cccgtccagc aggatatgct cggcctgaag
4201 gaaactctgg aaaagaggta cttcggcgag agcgctgatg gcaatgacaa tatttgtatc
4261 caggtgatcc ataacatcct ggacattgaa aaaatcctcg ccgaatacat taccaacgcc
4321 gcctacgccg tcaacaatat ctccggcctg gataaggaca ttattggatt cggcaagttc
4381 tccacagtgt atacctacga cgaattcaaa gaccccgagc accatagggc cgctttcaac
4441 aataacgata agctcatcaa cgccatcaag gcccagtatg acgagttcga caacttcctc
4501 gataacccca gactcggcta tttcggccag gcctttttca gcaaggaggg cagaaattac
4561 atcatcaatt acggcaacga atgctatgac attctggccc tcctgagcgg actggcgcac
4621 tgggtggtcg ctaacaacga agaagagtcc aggatctcca ggacctggct ctacaacctc
4681 gataagaacc tcgacaacga atacatctcc accctcaact acctctacga caggatcacc
4741 aatgagctga ccaactcctt ctccaagaac tccgccgcca acgtgaacta tattgccgaa
4801 actctgggaa tcaaccctgc cgaattcgcc gaacaatatt tcagattcag cattatgaaa
4861 gagcagaaaa acctcggatt caatatcacc aagctcaggg aagtgatgct ggacaggaag
4921 gatatgtccg agatcaggaa aaatcataag gtgttcgact ccatcaggac caaggtctac
4981 accatgatgg actttgtgat ttataggtat tacatcgaag aggatgccaa ggtggctgcc
5041 gccaataagt ccctccccga taatgagaag tccctgagcg agaaggatat ctttgtgatt
5101 aacctgaggg gctccttcaa cgacgaccag aaggatgccc tctactacga tgaagctaat
5161 agaatttgga gaaagctcga aaatatcatg cacaacatca aggaatttag gggaaacaag
5221 acaagagagt ataagaagaa ggacgcccct agactgccca gaatcctgcc cgctggccgt
5281 gatgtttccg ccttcagcaa actcatgtat gccctgacca tgttcctgga tggcaaggag
5341 atcaacgacc tcctgaccac cctgattaat aaattcgata acatccagag cttcctgaag
5401 gtgatgcctc tcatcggagt caacgctaag ttcgtggagg aatacgcctt tttcaaagac
5461 tccgccaaga tcgccgatga gctgaggctg atcaagtcct tcgctagaat gggagaacct
5521 attgccgatg ccaggagggc catgtatatc gacgccatcc gtattttagg aaccaacctg
5581 tcctatgatg agctcaaggc cctcgccgac accttttccc tggacgagaa cggaaacaag
5641 ctcaagaaag gcaagcacgg catgagaaat ttcattatta ataacgtgat cagcaataaa
5701 aggttccact acctgatcag atacggtgat cctgcccacc tccatgagat cgccaaaaac
5761 gaggccgtgg tgaagttcgt gctcggcagg atcgctgaca tccagaaaaa acagggccag
5821 aacggcaaga accagatcga caggtactac gaaacttgta tcggaaagga taagggcaag
5881 agcgtgagcg aaaaggtgga cgctctcaca aagatcatca ccggaatgaa ctacgaccaa
5941 ttcgacaaga aaaggagcgt cattgaggac accggcaggg aaaacgccga gagggagaag
6001 tttaaaaaga tcatcagcct gtacctcacc gtgatctacc acatcctcaa gaatattgtc
6061 aatatcaacg ccaggtacgt catcggattc cattgcgtcg agcgtgatgc tcaactgtac
6121 aaggagaaag gctacgacat caatctcaag aaactggaag agaagggatt cagctccgtc
6181 accaagctct gcgctggcat tgatgaaact gcccccgata agagaaagga cgtggaaaag
6241 gagatggctg aaagagccaa ggagagcatt gacagcctcg agagcgccaa ccccaagctg
6301 tatgccaatt acatcaaata cagcgacgag aagaaagccg aggagttcac caggcagatt
6361 aacagggaga aggccaaaac cgccctgaac gcctacctga ggaacaccaa gtggaatgtg
6421 atcatcaggg aggacctcct gagaattgac aacaagacat gtaccctgtt cgcaaacaag
6481 gccgtcgccc tggaagtggc caggtatgtc cacgcctata tcaacgacat tgccgaggtc
6541 aattcctact tccaactgta ccattacatc atgcagagaa ttatcatgaa tgagaggtac
6601 gagaaaagca gcggaaaggt gtccgagtac ttcgacgctg tgaatgacga gaagaagtac
6661 aacgataggc tcctgaaact gctgtgtgtg cctttcggct actgtatccc caggtttaag
6721 aacctgagca tcgaggccct gttcgatagg aacgaggccg ccaagttcga caaggagaaa
6781 aagaaggtgt ccggcaattc cggatccgga cctaagaaaa agaggaaggt ggcggccgct
6841 tacccatacg atgttccaga ttacgctgct agcggcagtg gagagggcag aggaagtctg
6901 ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga gcaagggcga ggagctgttc
6961 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
7021 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
7081 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
7141 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
7201 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
7261 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
7321 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
7381 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
7441 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
7501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
7561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
7621 atcactctcg gcatggacga gctgtacaag taagaattcg atatcaagct tatcgataat
7681 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
7741 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
7801 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
7861 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
7921 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
7981 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
8041 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
8101 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
8161 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
8221 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
8281 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
8341 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
8401 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
8461 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
8521 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
8581 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
8641 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
8701 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
8761 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
8821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
8881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
8941 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
9001 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
9061 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
9121 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
9181 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
9241 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
9301 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
9361 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
9421 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
9481 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
9541 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
9601 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
9661 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
9721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
9781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
9841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
9901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
9961 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
10021 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
10081 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
10141 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
10201 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
10261 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
10321 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
10381 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
10441 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
10501 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
10561 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
10621 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
10681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
10741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
10801 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
10861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
10921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
10981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
11041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
11101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
11161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
11221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
11281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
11341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
11401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
11461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
11521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
11581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
11641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
11701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
11761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
11821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
11881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
11941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
12001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
12061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
12121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
12181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
12241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
12301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
12361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
12421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
12481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
12541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
12601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
12661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
12721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
12781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
12841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
12901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
12961 catttccccg aaaagtgcca cctgacgtcg a
//
