| 质粒名称: | GFP-MAP1B |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 44396 |
| 存储实验室: | Phillip Gordon-Weeks |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 4731 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | MAP1B,microtubule-associated protein 1B |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | NM_008634.2 NP_032660.2 |
| 启动子: | CMV |
| 标签/融合蛋白: | GFP（主干上的N终端） |
| 克隆方法: | Ligation Independent克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | EBV_rev_primer |
|
文献引用方法 |
|
| 材料和方法部分: | GFP-MAP1B was a gift from Phillip Gordon-Weeks (Addgene plasmid # 44396 ; http://n2t.net/addgene:44396 ; RRID:Addgene_44396) |
| 参考文献部分: | Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR. J Cell Sci. 2009 Jul 15;122(Pt 14):2424-35. doi: 10.1242/jcs.040162. Epub 2009 Jun 23. 10.1242/jcs.040162 PubMed 19549690 |
买家导航
GFPMAP1B载体基本信息
GFPMAP1B载体图谱质粒图谱和多克隆位点信息
GFPMAP1B载体简介
GFPMAP1B载体序列
LOCUS GFP-MAP1B 12115 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS GFP-MAP1B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12115)
AUTHORS Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR
TITLE Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B
regulate microtubule dynamics in growing axons.
JOURNAL J Cell Sci. 2009 Jul 15;122(Pt 14):2424-35. doi: 10.1242/jcs.040162.
Epub 2009 Jun 23.
PUBMED 19549690
REFERENCE 2 (bases 1 to 12115)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12115
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 614..623
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 620..1336
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 620..1336
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(665..686)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(926..945)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1273..1294
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 8910..9031
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8947..8966)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9001..9020
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(9038..9493)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9175..9196)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9387..9406
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 9520..9624
/gene="bla"
/label=AmpR promoter
promoter 9626..9983
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(9649..9669)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 9834..9969
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9896..9915
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10018..10812
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10072..10091)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10682..10701
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(11000..11019)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 11044..11091
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 11420..12008
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11909..11928
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
781 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
1021 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
1081 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
1141 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
1201 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
1321 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
1381 catggcgacc gtggtggtgg aagccaccga accggagcca tcgggcagca tcggcaaccc
1441 ggcggcgtcc acctcgccca gcctgtcgca ccgcttccta gacagcaagt tctacttgct
1501 ggtggtggtc ggcgagaccg taaccgaaga gcacctgagg cgtgccatcg gcaacatcga
1561 gctggggatc cgatcgtggg acacaaacct gattgagtgc aacttggacc aggaactcaa
1621 acttttcgta tctcgacact ctgcaagatt ctctcctgaa gttccaggac aaaagattct
1681 tcatcaccga agtgacgtct tagaaacagt cgttctgatc aacccttcag atgaagccgt
1741 cagcactgag gtgcgtttga tgatcactga cgctgcccgc cataaactgc tggtgctcac
1801 cggacagtgc tttgagaaca ctggcgagct catcctgcag tctggctctt tctccttcca
1861 gaacttcata gagattttca cagatcaaga gattggtgag ctcctaagca ccacccaccc
1921 tgccaacaaa gccagcctca ccctcttctg tcccgaggag ggagactgga agaactccaa
1981 ccttgacaga cacaatctcc aagacttcat caacattaag ctcaactcag cttctatctt
2041 gccagaaatg gagggacttt ctgagttcac cgagtatctc tcagagtctg tagaagtccc
2101 ctctcccttt gacatcctgg aacccccaac ctcgggcggc tttctgaagc tctccaagcc
2161 ttgctgttac attttcccag ggggccgtgg ggactcggcc ctgtttgcag tgaacggatt
2221 caacatgctc atcaacggag gatcagagag aaagtcttgc ttctggaagc tcatccggca
2281 cttggaccgt gtggactcca tcctgctcac ccacattggg gatgacaatt tgcctgggat
2341 caatagcatg ctgcagcgca agattgcaga gctggaagag gagcggtccc aggggtccac
2401 cagcaacagt gactggatga aaaacctcat ctcccctgac ctgggggttg tgtttctcaa
2461 cgtacctgaa aacctgaaag acccagaacc caacatcaag atgaagagaa gcatagaaga
2521 agcatgcttc actctccagt acttaaacaa actgtccatg aaaccagagc ctctatttag
2581 aagtgtaggc aataccattg agcctgtcat cctgttccag aagatgggag tgggcaagct
2641 tgagatgtac gtgcttaacc cagtcaaaag cagcaaggaa atgcagtatt ttatgcaaca
2701 gtggactggc accaacaaag acaaggccga actaatcctg cccaatggtc aagaagtaga
2761 catcccgatt tcctacttaa cctcagtctc atctttgatt gtgtggcatc cggccaaccc
2821 cgctgagaaa atcattcggg ttctgtttcc cggaaacagt acccagtaca acatcctaga
2881 aggactggag aaactcaaac atctagattt cctaaagcag ccactggcca cccaaaaaga
2941 tctcactggc caggtgccca cccccccagt gaaacaggtc aaactgaaac agcgggctga
3001 cagccgagag agtctgaagc cagccacgaa accagttgcc agtaaatctg tgaggaagga
3061 gtccaaggag gaaacccctg aagtcacaaa gactagtcaa gtggagaaaa cacccaaagt
3121 tgaaagcaaa gagaaagtgt tagtgaaaaa agacaagcct gtaaagacag aaagtaaacc
3181 ttcagtgaca gaaaaggagg tgtccagcaa ggaagagcag tccccagtga aagccgaggt
3241 cgctgagaag caagccactg agagcaaacc caaagtcacc aaggacaaag tggtaaaaaa
3301 ggaaataaag acaaaactcg aggaaaagaa agaggagaag cccaagaaag aagtggttaa
3361 gaaggaagac aaaacacccc tcaagaaaga cgagaagcca agaaaggaag aggtgaagaa
3421 ggagatcaag aaagaaatca aaaaagaaga gagaaaggag ctgaagaaag aggtgaagaa
3481 ggaaacaccc ctgaaggatg ccaagaagga ggtgaagaaa gaggagaaga aagaagttaa
3541 aaaggaagag aaggaaccca aaaaggaaat caagaagatc tccaaggaca taaagaaatc
3601 aactcctctg tcagacacaa agaagccatc tgcgttaaaa ccaaaagtag cgaagaaaga
3661 agagtccact aagaaggagc ctcttgctgc tgggaaactc aaggacaagg ggaaagtcaa
3721 agtcattaaa aaggaaggca agaccacaga agcagctgcc acagctgttg gcactgctgc
3781 caccacagct gccgtggtgg ctgcagctgg aatagcggcc agtggtcctg tcaaagaact
3841 ggaagctgag aggtccctta tgtcctcccc tgaggatcta accaaggact ttgaggagct
3901 aaaggctgag gagattgatg tggccaagga catcaagcct cagctggagc tcattgaaga
3961 tgaagagaaa ctgaaggaaa cccagccggg agaagcctac gtcatccaga aagagacaga
4021 agtcagcaaa ggttccgctg agtcacccga tgagggaatc accaccaccg agggggaggg
4081 cgagtgtgag caaacccctg aggagctgga gccagttgag aagcagggcg tggatgacat
4141 tgagaagttt gaagatgaag gagctggttt tgaagaatcc tcagagaccg gagactacga
4201 agagaaggcg gaaactgagg aggctgagga accggaagag gacggggaag acaatgcgag
4261 cgggagtgcc tctaagcaca gccccacaga agatgacgaa agcgcaaagg ctgaggcgga
4321 cgtgcacctc aaggagaaga gggagtctgt ggtcagtggt gacgaccggg cggaagaaga
4381 catggatgat gtgcttgaga aaggagaagc tgaacagtct gaggaagagg gcgaagagga
4441 ggacaaagca gaggatgcca gagaggaagg ctatgagccc gacaaaactg aggctgagga
4501 ttatgtgatg gccgtggctg acaaggcggc tgaggctgga gtcacagagg agcagtacgg
4561 atacctgggc acgtcggcca agcaacctgg aatccagtct cctagccgag aacctgcatc
4621 ttcaattcat gatgagaccc tacctggagg ctctgagagc gaggccactg cttcggatga
4681 ggagaatagg gaagaccagc ctgaggaatt cactgctacc tctggatata ctcagtctac
4741 cattgagata tctagtgagc ctacgccaat ggatgagatg tctactcctc gtgatgtgat
4801 gagcgacgag accaacaacg aggagacaga gtccccatct caggagttcg taaacattac
4861 caaatatgag tcctcgctgt attctcagga gtactccaaa cctgctgttg catcattcaa
4921 tggattgtca gaagggtcaa aaacagacgc cactgatggt aaggattaca acgcttccgc
4981 ctccaccata tcaccacctt cgtccatgga agaagacaaa ttcagcaagt ctgctcttcg
5041 tgatgcttac tgctcagaag agaaagagtt gaaagccagt gccgagttag acatcaaaga
5101 tgtttcagat gagagactta gcccagccaa gagtccatcc ctgagtcctt ctccgccatc
5161 ccccatagag aagactcccc tgggtgaacg tagcgtgaat ttctctctga cacccaatga
5221 gattaaagtg tcagcagagg gagaagcaag gtcagtgtcc cctggagtga cccaggcagt
5281 agttgaagaa cattgtgcca gtcctgagga gaagaccctg gaagtagtgt cgccatccca
5341 gtctgtgaca ggcagtgctg gccacacacc ttactaccaa tctcccaccg atgagaagtc
5401 cagtcaccta cccacagaag tcactgaaaa gccacaggca gttccagtga gctttgaatt
5461 cagtgaggcc aaagatgaga acgagagagc gtccctaagc cccatggatg agcctgtgcc
5521 tgactcagag tctcctgtgg agaaggttct gtctccttta cgcagtcctc cccttcttgg
5581 atctgagtcc ccatatgaag acttcctgag tgctgatagc aaggttcttg gcagacgttc
5641 cgaaagcccc tttgaaggga agaatggaaa gcagggcttc ccagacagag aaagcccagt
5701 ttctgacctg acttccactg gtctttacca agacaaacag gaagagaaaa gcacgggctt
5761 catacccata aaggaagact ttggtccaga aaagaaaacc agtgatgttg aaaccatgag
5821 ttctcaatca gctctagctt tggatgaaag gaaattggga ggagatgtat ctccaacaca
5881 aatagacgtc agtcagtttg gctctttcaa agaagacacc aagatgtcca tttcagaagg
5941 cactgtttca gacaagtcag ccacacctgt ggatgagggt gtggcagaag acacctactc
6001 ccacatggag ggtgtggcct ccgtctctac tgcctctgtg gctacaagtt cgtttccaga
6061 gccaaccaca gatgacgtgt ctccttctct gcatgccgag gtgggttctc cacattccac
6121 agaagttgat gactccctgt ccgtttctgt ggtgcaaaca cctaccacgt tccaggaaac
6181 agaaatgtct ccatctaaag aagagtgccc aagaccgatg tcaatttctc ctccagactt
6241 ctcccccaag acagccaaat ccaggacacc agttcaagat caccgatctg aacagtcttc
6301 tatgtctatt gagttcggcc aggaatcccc tgagcattct tttgctatgg actttagtcg
6361 gcagtctcca gatcacccta ctctgggtgc cagtgtgctt catatcactg aaaacgggcc
6421 aactgaagtg gactatagtc cctctgatat ccaggactct agtttgtcgc ataagatccc
6481 gccaacggaa gagccatcct acacccagga taatgatctg tctgagctca tctctgtgtc
6541 tcaggtagag gcttccccgt ccacctcttc agctcacacc ccttctcaga tagcctctcc
6601 tcttcaggaa gacactctct ctgatgttgt tcctcccaga gagatgtcct tatatgcctc
6661 acttgcgtct gaaaaagtgc agagcctgga aggagagaag ctctctccga aatctgatat
6721 ttctccactc accccacgag agtcctcacc tttatattca cctggctttt cagattctac
6781 ctctgcagct aaagagactg cagcagctca ccaagcctcc tcttccccac caatagatgc
6841 agcaacagca gagccctatg gcttccgctc ctcaatgcta tttgatacaa tgcaacatca
6901 tctggccttg aatagagatt tgactacatc tagtgtggag aaagacagcg gagggaagac
6961 acccggagac tttaactatg cctatcaaaa gcctgagaat gccgccggat ctccagatga
7021 agaagattat gactatgaat ctcaagagaa aaccatccgg acccacgacg tgggtggtta
7081 ctactatgag aagacagaga gaaccataaa atccccatgt gacagtgggt actcctatga
7141 gaccattgag aagaccacca agaccccaga agatggtggc tacacctgtg aaattaccga
7201 gaaaaccact cggaccccag aagagggtgg ctattcatat gagatcagcg agaagacgac
7261 acggacccct gaagtaagcg gctacaccta tgaaaagact gagaggtcca gaaggctcct
7321 ggatgacatt agcaatggct atgatgacac tgaggatggc ggccacacac ttggcgactg
7381 tagctactcc tatgaaacca ctgagaaaat taccagcttt cctgagtctg aaagctattc
7441 ctacgagaca agtacaaaaa caacacggag tccagacacc tctgcatact gttacgagac
7501 catggagaag atcaccaaga ccccacaggc atccacatac tcctatgaga cctcagaccg
7561 atgctacact acggaaaaga agtccccctc agaggcacgc caggatgttg atttatgtct
7621 ggtgtcctcc tgtgaattca agcatcccaa gacggagctc tcgccttcct tcattaatcc
7681 aaatcctctt gagtggtttg ctggtgaaga gcccactgaa gaatcagaga agcccctcac
7741 tcagtctgga ggagcccccc caccttcagg aggaaaacaa cagggcaggc aatgcgatga
7801 aacgccaccc acctcagtca gtgagtcagc cccatcccag acagactctg atgttccccc
7861 agagacggaa gagtgcccct ccatcacagc cgacgccaat attgactctg aagatgagtc
7921 agaaactatc cccacagaca aaaccgtcac atacaaacac atggacccac ctccagcccc
7981 catgcaagac cgaagccctt ctcctcgcca ccccgacgtg tccatggtgg acccagacgc
8041 cttggcagtt gatcagaacc taggcaaggc tctgaaaaaa gacctgaagg agaagaccaa
8101 gaccaagaag ccaggcacaa agaccaagtc ctcttcacct gtcaaaaagg gtgatgggaa
8161 gtccaagcct ttagcagctt cccccaaacc aggagctttg aaggaatcct ccgacaaggt
8221 gtccagagtg gcttctccca agaagaaaga atccgtggaa aaagctacaa agaccaccac
8281 cactcctgag gtcaaagcca cacgcggcga agagaaggac aaggagacca agaacgcagc
8341 taacgcctct gcatccaaat cagcgaagac cgcaacgaca ggaccaggaa ccactaagac
8401 agccaagtcg tccaccgtgc ctccaggcct ccctgtgtat ttggacctgt gctatattcc
8461 caaccacagc aacagtaaga atgtcgatgt tgagttcttc aagagagtga ggtcatctta
8521 ctacgtggtg agtgggaacg accctgccgc ggaggagccc agccgggctg tcctggatgc
8581 cttgttggaa gggaaggctc agtggggaag caacatgcag gtgactctga ttccgacaca
8641 tgactccgag gtgatgaggg agtggtacca ggagacccac gagaagcagc aggacctcaa
8701 catcatggtc ttagcaagca gcagtacagt ggtcatgcaa gacgagtcct tccccgcatg
8761 caagatagaa ctgtagcccg ggatccaccg gatctagata actgatcata atcagccata
8821 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
8881 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
8941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
9001 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
9061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
9121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
9181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
9241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
9301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
9361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
9421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
9481 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
9541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
9601 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
9661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
9721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
9781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
9841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
9901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
9961 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
10021 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
10081 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
10141 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
10201 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
10261 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
10321 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
10381 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
10441 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
10501 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
10561 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
10621 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
10681 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
10741 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
10801 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
10861 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
10921 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
10981 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
11041 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
11101 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
11161 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
11221 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
11281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
11341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
11401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
11461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
11521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
11581 cgtagttagg ccaccacttc aagaactctg tatcaccgcc tacatacctc gctctgctaa
11641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
11701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
11761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
11821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
11881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
11941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
12001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
12061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgc
//
GFPMAP1B载体图谱质粒图谱pdf版和相关资料下载
GFPMAP1B载体质粒应用举例
- 上一篇：pDONR221_SLC22A31
- 下一篇：pNO183
