pHOOK1

价格:3850元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pHOOK1
质粒类型: 哺乳动物表达载体;Capture-Tec系统;免疫磁珠
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6061 bp
5' 测序引物及序列: CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: IgK、HA tag (N-term);2XMyc tag (N-term)
载体抗性: 氨苄青霉素
筛选标记: 博来霉素
克隆菌株: DH5α
宿主细胞(系): 哺乳动物细胞
备注:
pHOOK1载体可以用于:  
 1.       The effect of dominant, negative mutations of growth regulating proteins or toxic gene products
2.       The acute effects of constitutive mutations
3.       The effect of anti-sense RNA or ribozymes
4.       Temporal events along a developmental or signaling pathway
5.       Activation or inactivation of downstream genes using differential display
6.       You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for:
        a)        Immunoblot analysis of proteins
        b)        Isolation of DNA or RNA
        c)         Flow Cytometry

See pHOOK-3
 
产品目录号: V690-20
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pHOOK1载体图谱
pHOOk1多克隆位点

pEBVHis载体特征

载体简介

载体序列

LOCUS       Exported                6061 bp ds-DNA     circular SYN 12-AUG-2016
KEYWORDS    pHOOK1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Biofeng Lab.
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        10..389
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        638..656
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    664..732
                     /note="MCS"
     CDS             737..799
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /note="Ig-kappa leader"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             800..826
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    827..1564
                     /note="phOx sFv"
     CDS             1565..1594
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             1610..1639
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     polyA_signal    1817..2041
                     /note="bGH poly(A)"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2173..2761)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3090..3137
                     /note="HSV TK poly(A)"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(4192..4552)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      4206..4344
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             complement(4612..5472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5473..5577)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      5604..6059
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgact atccatatga tgttccagat tatgctgggg cccagccggc
      841 catggccgag gtcaagctgc aggagtcagg gggaggctta gtgcagcctg gagggtcccg
      901 gaaactctcc tgtgcagcct ctggattcac tttcagtagc tttggaatgc actgggttcg
      961 tcaggctcca gagaaggggc tggagtgggt cgcatatatt agtagtggca gtagtaccat
     1021 ctactatgca gacacagtga agggacgatt caccatctcc agagacaatc ccaagaacac
     1081 cctgttcctg caaatgacca gtctaaggtc tgaggacacg gncatgtatt actgtgcaag
     1141 agattacggg gcttattggg gccaagggac cacggncacc gtctcctcag gtggaggcgg
     1201 ctcaggcgga ggtggctctg gcggtggcgg atcggacatt gagctcaccc agtctccagc
     1261 aatcatgtct gcatctccag gggagagggt caccatgacc tgcagtgcca gttcaagtgt
     1321 aaggtacatg aactggttcc aacagaagtc aggcacctcc cccaaaagat ggatttatga
     1381 cacatccaaa ctgtcttctg gagtccctgc tcgcttcagt ggcagtgggt ctgggacctc
     1441 ttactctctc acaatcagca gcatggaggc tgaagatgct gccacttact actgccagca
     1501 gtggagtagt aacccactca cgttcggtgc tgggaccaag ctggagctga aacgggcggc
     1561 cgcagaacaa aaactcatct cagaagagga tctgaatggg gccgtcgacg aacaaaaact
     1621 catctcagaa gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc
     1681 acactccttg ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac
     1741 catcatctcc cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc
     1801 tcgagatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa
     2101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2581 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2881 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     2941 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     3001 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     3061 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     3121 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     3181 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     3241 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     3301 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     3361 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     3421 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     3481 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     3541 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     3601 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     3661 gctggcgcga gcccctgatg ctcttgatca tcctgatcga caagaccggc ttccatccga
     3721 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     3781 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     3841 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     3901 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     3961 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     4021 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     4081 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     4141 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     4201 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggaggc
     4261 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     4321 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     4381 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     4441 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     4501 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     4561 ctttttcaat aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5581 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6001 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     6061 c
//

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