GW1-Peredox-mCherry-NLS

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: GW1-Peredox-mCherry-NLS 
出品公司: 爱迪基因
目录编号: 32381
存储实验室: Gary Yellen

载体骨架基本信息
载体名称: GW1
载体出品公司: British Biotechnology
载体抗性: 氨苄霉素
空载体大小: 5300 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Peredox-mCherry-NLS
插入大小: 2800 bp
启动子 CMV
标签/融合蛋白: Nuclear Localization Seq(C端)
克隆方法: 限制性内切酶
5' 克隆位点: PspOMI(未破坏)
3' 克隆位点: SalI(未破坏)
5' 测序引物 CMV-F
3' 测序引物 SV40pA-R

文献引用方法
材料和方法部分: GW1-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32381)
参考文献部分: Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714

载体图谱质粒图谱和多克隆位点信息

GW1-Peredox-mCherry-NLS载体图谱

载体简介

载体序列

LOCUS       GW1-Peredox-mCherry-NLS	8075 bp 	DNA	SYN
DEFINITION  GW1-Peredox-mCherry-NLS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="GW1-Peredox-mCherry-NLS"
                     /mol_type="other DNA"
     CDS             121..2919
                     /label="ORF frame 1"
     CDS             complement(934..1485)
                     /label="ORF frame 3"
     CDS             complement(1683..2246)
                     /label="ORF frame 1"
     CDS             complement(1978..2940)
                     /label="ORF frame 3"
     gene            2182..2886
                     /label="mCherry"
                     /gene="mCherry"
     misc_feature    2359..2412
                     /label="Q66M (mCherry)"
     misc_feature    2887..2907
                     /label="SV40 NLS"
     misc_feature    2893..2907
                     /label="NLS"
     terminator      3044..3175
                     /label="SV40_PA_terminator"
     misc_feature    3132..3151
                     /label="EBV_rev_primer"
     promoter        complement(3195..3213)
                     /label="M13_reverse_primer"
     misc_feature    complement(3212..3234)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3248..3277)
                     /label="lac_promoter"
     rep_origin      complement(3586..4205)
                     /label="pBR322_origin"
     gene            complement(4360..5220)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4360..5220)
                     /label="ORF frame 3"
     promoter        complement(5299..5327)
                     /label="AmpR_promoter"
     misc_feature    complement(5486..5508)
                     /label="pGEX_3_primer"
     misc_feature    complement(5695..5714)
                     /label="SV40pro_F_primer"
     misc_feature    complement(5745..5888)
                     /label="lacZ_a"
     CDS             complement(5745..6272)
                     /label="ORF frame 1"
     misc_feature    5859..5881
                     /label="M13_pUC_fwd_primer"
     promoter        5874..5890
                     /label="M13_forward20_primer"
     promoter        6440..7016
                     /label="CMV_immearly_promoter"
     misc_feature    6519..6806
                     /label="CAG_enhancer"
     misc_feature    6973..6993
                     /label="CMV_fwd_primer"
     promoter        6974..7043
                     /label="CMV_promoter"
     promoter        6986..7105
                     /label="CMV2_promoter"
     misc_feature    7017..7036
                     /label="pCEP_fwd_primer"
     misc_feature    7019..7043
                     /label="LNCX_primer"
     CDS             complement(7511..7969)
                     /label="ORF frame 2"
     CDS             complement(7872..762)
                     /label="ORF frame 3"
ORIGIN
    1 tccgagtccg ataccgataa agattctgaa tcggagtgag tgccgcgtcc gagagcgttc
   61 cctgtccacg tccaccatcg gcggagcagg tgtgcctgag gcctctagat tgggcccacc
  121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
  181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
  241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
  301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
  361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
  421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
  481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
  541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
  601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
  661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
  721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
  781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
  841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
  901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
  961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
 1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
 1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
 1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
 1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
 1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
 1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
 1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
 1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
 1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
 1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
 1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
 1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
 1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
 1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
 1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
 1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
 1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
 2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
 2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
 2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
 2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
 2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
 2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
 2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
 2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
 2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
 2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
 2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
 2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
 2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
 2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
 2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgacgaatt cattgatcat
 2941 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
 3001 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
 3061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
 3121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgtac
 3181 cgagctcgcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
 3241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
 3301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
 3361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
 3421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
 3481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
 3541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
 3601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
 3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
 3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
 3781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
 3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
 3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
 3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
 4021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
 4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
 4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
 4201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
 4261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
 4321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
 4381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
 4441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
 4501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
 4561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
 4621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
 4681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
 4741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
 4801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
 4861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
 4921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
 4981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
 5041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
 5101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
 5161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
 5221 actcttcctt tttcaataag cggccgcggc catgccggcc actagtctcg agttattatt
 5281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
 5341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
 5401 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
 5461 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
 5521 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
 5581 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
 5641 atatgaagac gtcgcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
 5701 cctctgcata aataaaaaaa attagtcagc gcgccattcg ccattcaggc tgcgcaactg
 5761 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
 5821 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
 5881 gacggccagt gccatctgca gtgaataata aaatgtgtgt ttgtccgaaa tacgcgtttg
 5941 agatttctgt cccgactaaa ttcatgtcgc gcgatagtgg tgtttatcgc cgatagagat
 6001 ggcgatattg gaaaaatcga tatttgaaaa tatggcatat tgaaaatgtc gccgatgtga
 6061 gtttctgtgt aactgatatc gccatttttc caaaagttga tttttgggca tacgcgatat
 6121 ctggcgatac gcttatatcg tttacggggg atggcgatag acgcctttgg tgacttgggc
 6181 gattctgtgt gtcgcaaata tcgcagtttc gatataggtg acagacgata tgaggctata
 6241 tcgccgatag aggcgacatc aagctggcac atggccaatg catatcgatc tatacattga
 6301 atcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
 6361 attggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
 6421 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
 6481 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
 6541 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
 6601 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
 6661 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
 6721 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
 6781 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
 6841 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
 6901 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
 6961 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
 7021 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
 7081 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
 7141 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
 7201 cttatgcatg ctatactgtt tttggcttgg ggtctataca cccccgcttc ctcatgttat
 7261 aggtgatggt atagcttagc ctataggtgt gggttattga ccattattga ccactcccct
 7321 attggtgacg atactttcca ttactaatcc ataacatggc tctttgccac aactctcttt
 7381 attggctata tgccaataca ctgtccttca gagactgaca cggactctgt atttttacag
 7441 gatggggtct catttattat ttacaaattc acatatacaa caccaccgtc cccagtgccc
 7501 gcagttttta ttaaacataa cgtgggatct ccacgcgaat ctcgggtacg tgttccggac
 7561 atgggctctt ctccggtagc ggcggagctt ctacatccga gccctgctcc catgcctcca
 7621 gcgactcatg gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca
 7681 gcacgatgcc caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg
 7741 aaaatgagct cggggagcgg gcttgcaccg ctgacgcatt tggaagactt aaggcagcgg
 7801 cagaagaaga tgcaggcagc tgagttgttg tgttctgata agagtcagag gtaactcccg
 7861 ttgcggtgct gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc
 7921 gcgccaccag acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct
 7981 gcagtcaccg tccttgacac gaagcttccc ggccacagca atcgatccat gacacaacca
 8041 gtgacaagca gtcccccagt gaaaccgcat ccgca
//

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