pIZT/V5-His

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pIZT/V5-His
质粒类型: 昆虫表达载体;荧光报告载体
克隆方法: 多克隆位点,限制性内切酶
启动子: OplE2
载体大小: 3336 bp
5' 测序引物及序列: OpIE2 Forward:CGCAACGATCTGGTAAACAC
3' 测序引物及序列: --
载体标签: V5 Epitope Tag , His Tag (C-端)
载体抗性: Zeocin
真核筛选标记: Zeocin;GFP
克隆菌株: TOP10;DH5alpha
宿主细胞(系): Sf9, High Five
备注: pIZT/V5-His载体含有荧光报告基因GFP,与抗性基因Zeocin融合表达,即Zeocin-GFP融合蛋白的形式;
表达水平高。
产品目录号: V8010-01
瞬表达/稳表达: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pIZT-V5-His载体图谱



pIZT-V5-His 多克隆位点

pIZT-V5-His 载体特征

载体简介

The InsectSelect Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:

• The OpIE2 promoter for constitutive expression
• The Zeocin resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells.

Promoter:	OplE2
Vector Type:	pIZ
Product Size:	1 kit
Cloning Method:	Restriction Enzyme ⁄ MCS
Protein Tag or Fusion:	V5 Epitope Tag, His Tag (6x)
Protein Tag Position (to your gene):	C-terminal

载体序列

LOCUS       pIZT/V5-His	3336 bp 	DNA	SYN
DEFINITION  pIZT/V5-His
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="pIZT/V5-His"
                     /mol_type="other DNA"
     misc_feature    515..534
                     /label="OpIE2_fwd_primer"
     rep_origin      complement(991..1610)
                     /label="pBR322_origin"
     promoter        1984..2051
                     /label="EM7_promoter"
     CDS             2052..3143
                     /label="ORF frame 3"
     gene            2076..2771
                     /label="GFP (0 - 695)"
                     /gene="GFP (0 - 695)"
     misc_feature    complement(2100..2128)
                     /label="GFP_R_primer"
     misc_feature    complement(2144..2163)
                     /label="GFPfusionREV_primer"
     misc_feature    2723..2743
                     /label="GFP_F_primer"
     gene            2781..3140
                     /label="bleo"
                     /gene="bleo"
     misc_feature    2781..3143
                     /label="sh_ble"
     terminator      3210..5
                     /label="SV40_PA_terminator"
     misc_feature    3298..3317
                     /label="EBV_rev_primer"
ORIGIN
    1 GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC
   61 TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT
  121 TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG
  181 TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC
  241 ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT
  301 TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA
  361 TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG
  421 AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC
  481 GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA
  541 ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT
  601 GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG
  661 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC
  721 ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA
  781 TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA
  841 ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC
  901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
  961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
 1021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
 1081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
 1141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
 1201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
 1261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
 1321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
 1381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
 1441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
 1501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
 1561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
 1621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC GAAACACGCA
 1681 CGGCGCGCGC ACGCAGCTTA GCACAAACGC GTCGTTGCAC GCGCCCACCG CTAACCGCAG
 1741 GCCAATCGGT CGGCCGGCCT CATATCCGCT CACCAGCCGC GTCCTATCGG GCGCGGCTTC
 1801 CGCGCCCATT TTGAATAAAT AAACGATAAC GCCGTTGGTG GCGTGAGGCA TGTAAAAGGT
 1861 TACATCATTA TCTTGTTCGC CATCCGGTTG GTATAAATAG ACGTTCATGT TGGTTTTTGT
 1921 TTCAGTTGCA AGTTGGCTGC GGCGCGCGCA GCACCTTTGC CGGGATCTGC CGGGCTGCAG
 1981 CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA
 2041 GGAACTAAAC CATGGCGGTA GAAAAAATGG CTAGCAAAGG AGAAGAACTT TTCACTGGAG
 2101 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG
 2161 GAGAGGGTGA AGGTGATGCT ACATACGGAA AGCTTACCCT TAAATTTATT TGCACTACTG
 2221 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT
 2281 TTTCCCGTTA TCCGGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
 2341 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAACTACAAG ACGCGTGCTG
 2401 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAAGGT ATTGATTTTA
 2461 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTATAACTCA CACAATGTAT
 2521 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCAAAATT CGTCACAACA
 2581 TTGAAGATGG ATCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG
 2641 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCGACACA ATCTGCCCTT TCGAAAGATC
 2701 CCAACGAAAA GCGTGACCAC ATGGTCCTTC TTGAGTTTGT AACTGCTGCT GGGATTACAC
 2761 ATGGCATGGA TGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG
 2821 GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT
 2881 TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG
 2941 TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT
 3001 GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG
 3061 GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT
 3121 TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC CGCCGGTCCG ACGCGGCCCG
 3181 ACGGGTCCGA GGGGGGTCGA CCTCGAAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT
 3241 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG
 3301 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT
//

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