| 质粒名称: | MP6 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69669 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CloDF13 |
| 载体出品公司: | David Liu Lab |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 2992 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dnaQ926 dam seqA emrR ugi CDA1 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | E. coli PBAD |
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pBAD-F |
| 3' 测序引物 | CAT-F |
|
文献引用方法 |
|
| 材料和方法部分: | MP6 was a gift from David Liu (Addgene plasmid # 69669 ; http://n2t.net/addgene:69669 ; RRID:Addgene_69669) |
| 参考文献部分: | Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MP6 6681 bp ds-DNA circular SYN 28-AUG-2018
DEFINITION Untargetted Bacterial Mutagenesis.
ACCESSION .
VERSION .
KEYWORDS MP6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6681)
AUTHORS Badran AH, Liu DR
TITLE Development of potent in vivo mutagenesis plasmids with broad
mutational spectra.
JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
PUBMED 26443021
REFERENCE 2 (bases 1 to 6681)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 28, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6681
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 208..227
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS 3093..3347
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 3377..4003
/codon_start=1
/product="cytidine deaminase from the sea lamprey
Petromyzon marinus (Rogozin et al., 2007)"
/label=PmCDA1
/translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
terminator 4030..4061
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(4077..4736)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
rep_origin complement(4889..5627)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
terminator 5717..5762
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(5777..6655)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
301 cgcgtacaac tcaagtctga cataaatgac cgctatgagc actgcaatta cacgccagat
361 cgttctcgct accgcaacca ccggtatgaa ccagattggt gcgcactatg aaggccacaa
421 gatcattgag attggtgccg ttgaagtggt gaaccgtcgc ctgacgggca ataacttcca
481 tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac atggtattgc
541 cgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt tcatggacta
601 tattcgcggc gcggagttgg tgatccataa cgcagcgttc gatatcggct ttatggacta
661 cgagttttcg ttgcttaagc gcgatattcc gaagaccaat actttctgta aggtcaccga
721 tagccttgcg gtggcgagga aaatgtttcc cggtaagcgc aacagcctcg atgcgttatg
781 tgctcgctac gaaatagata acagtaaacg aacgctgcac ggggcattac tcgatgccca
841 gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg cttttgcgat
901 ggaaggagag acacaacagc aacaaggtga agcaacaatt cagcgcattg tacgtcaggc
961 aagtaagtta cgcgttgttt ttgcgacaga tgaagagatt gcagctcatg aagcccgtct
1021 cgatctggtg cagaagaaag gcggaagttg cctctggcga gcataattta atatcagtaa
1081 accggacata acccatgaag aaaaatcgcg cttttttgaa gtgggcaggg ggcaagtatc
1141 ccctgcttga tgatattaaa cggcatttgc ccaagggcga atgtctggtt gagccttttg
1201 taggtgccgg gtcggtgttt ctcaacaccg acttttctcg ttatatcctt gccgatatca
1261 atagcgacct gatcagtctc tataacattg tgaagatgcg tactgatgag tacgtacagg
1321 ccgcacgcga gctgtttgtt cccgaaacaa attgcgccga ggtttactat cagttccgcg
1381 aagagttcaa caaaagccag gatccgttcc gtcgggcggt actgttttta tatttgaacc
1441 gctacggtta caacggcctg tgtcgttaca atctgcgcgg tgagtttaac gtgccgttcg
1501 gccgctacaa aaaaccctat ttcccggaag cagagttgta tcacttcgct gaaaaagcgc
1561 agaatgcctt tttctattgt gagtcttacg ccgatagcat ggcgcgcgca gatgatgcat
1621 ccgtcgtcta ttgcgatccg ccttatgcac cgctgtctgc gaccgccaac tttacggcgt
1681 atcacacaaa cagttttacg cttgaacaac aagcgcatct ggcggagatc gccgaaggtc
1741 tggttgagcg ccatattcca gtgctgatct ccaatcacga tacgatgtta acgcgtgagt
1801 ggtatcagcg cgcaaaattg catgtcgtca aagttcgacg cagtataagc agcaacggcg
1861 gcacacgtaa aaaggtggac gaactgctgg ctttgtacaa accaggagtc gtttcacccg
1921 cgaaaaaata attcagctaa gacactgcac tggattaaga tgaaaacgat tgaagttgat
1981 gatgaactct acagctatat tgccagccac actaagcata tcggcgagag cgcatccgac
2041 attttacggc gtatgttgaa attttccgcc gcatcacagc ctgctgctcc ggtgacgaaa
2101 gaggttcgcg ttgcgtcacc tgctatcgtc gaagcgaagc cggtcaaaac gattaaagac
2161 aaggttcgcg caatgcgtga acttctgctt tcggatgaat acgcagagca aaagcgagcg
2221 gtcaatcgct ttatgctgct gttgtctaca ctatattctc ttgacgccca ggcgtttgcc
2281 gaagcaacgg aatcgttgca cggtcgtaca cgcgtttact ttgcggcaga tgaacaaacg
2341 ctgctgaaaa atggtaatca gaccaagccg aaacatgtgc caggcacgcc gtattgggtg
2401 atcaccaaca ccaacaccgg ccgtaaatgc agcatgatcg aacacatcat gcagtcgatg
2461 caattcccgg cggaattgat tgagaaggtt tgcggaacta tctaacggct gaaattaatg
2521 aggtcatacc caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc
2581 cagccgccac gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcacat
2641 gcaaagcaag ctgctggaga accgcaataa aatgctgaag gctcagggaa ttaacgagac
2701 gttgtttatg gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga
2761 attaagttgt gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga
2821 aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca
2881 attaacggaa aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct
2941 gcatcaactc tggtccgcgc tcagcacaac agaaaaagat cagctcgagc aaatcacccg
3001 caaattgctc tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag
3061 ctaataatac aaaaattagg aggaatttca acatgacaaa tttatctgac atcattgaaa
3121 aagaaacagg aaaacaacta gtgattcaag aatcaattct aatgttacca gaagaagtag
3181 aggaagtaat tgggaataaa ccagaaagtg atattttagt tcatactgct tatgatgaaa
3241 gtacagatga aaatgtaatg ctattaactt cagatgctcc agaatataaa ccttgggctt
3301 tagtaattca agacagtaat ggagaaaata aaattaaaat gttataagtc gagattaagt
3361 aaaccggaat ctgaagatga ccgacgcgga atacgttcgt atccacgaaa aactggacat
3421 ctacaccttc aaaaaacagt tcttcaacaa caaaaaatct gtttctcacc gttgctacgt
3481 tctgttcgaa ctgaaacgtc gtggtgaacg tcgtgcgtgc ttctggggtt acgcggttaa
3541 caaaccgcag tctggtaccg aacgtggtat ccacgcggaa atcttctcta tccgtaaagt
3601 tgaagaatac ctgcgtgaca acccgggtca gttcaccatc aactggtact cttcttggtc
3661 tccgtgcgcg gactgcgcgg aaaaaatcct ggaatggtac aaccaggaac tgcgtggtaa
3721 cggtcacacc ctgaaaatct gggcgtgcaa actgtactac gaaaaaaacg cgcgtaacca
3781 gatcggtctg tggaacctgc gtgacaacgg tgttggtctg aacgttatgg tttctgaaca
3841 ctaccagtgc tgccgtaaaa tcttcatcca gtcttctcac aaccagctga acgaaaaccg
3901 ttggctggaa aaaaccctga aacgtgcgga aaaacgtcgt tctgaactgt ctatcatgat
3961 ccaggttaaa atcctgcaca ccaccaaatc tccggcggtt taaacttaat taacggcact
4021 cctcagccaa gtcaaaagcc tccggtcgga ggcttttgac tacatgccca tggcgtttac
4081 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
4141 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
4201 tgcgtataat atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg
4261 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
4321 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
4381 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
4441 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
4501 tctttcattg ccatacggaa ctccggatga gcattcatca ggcgggcaag aatgtgaata
4561 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
4621 agctgaacgg tctggttata ggtacattga gtaactgact gaaatgcctc aaaatgttct
4681 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
4741 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
4801 tcattatggt gaaagttgga acctcttacg tgccaagcca aataggccgt cactcggtcg
4861 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
4921 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg
4981 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca
5041 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
5101 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct
5161 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat
5221 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
5281 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
5341 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
5401 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
5461 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
5521 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
5581 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
5641 cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agcccaggag
5701 gaagaggaca tccggtcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
5761 tagacttagg gaccctttat gacaacttga cggctacatc attcactttt tcttcacaac
5821 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga
5881 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
5941 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
6001 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
6061 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
6121 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
6181 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
6241 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
6301 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
6361 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
6421 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
6481 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
6541 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
6601 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
6661 tcatactccc accattcaga g
//
