| 质粒名称: | pAc-sgRNA-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 49330 |
| 存储实验室: | Ji-Long Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pAc-STABLE1-Puro |
| 载体出品公司: | Dr. James Sutherland |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6600 bp |
| 完整质粒大小: | 10658 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | -- |
| 插入大小: | 4278 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pAc-sgRNA-Cas9 was a gift from Ji-Long Liu (Addgene plasmid # 49330 ; http://n2t.net/addgene:49330 ; RRID:Addgene_49330) |
| 参考文献部分: | Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Bassett AR, Tibbit C, Ponting CP, Liu JL. Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120. 10.1242/bio.20137120 PubMed 24326186 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAc-sgRNA-Cas9 10632 bp ds-DNA circular SYN 12-NOV-2018
DEFINITION Expresses sgRNA and Cas9-Puro in Drosophila S2 cells.
ACCESSION .
VERSION .
KEYWORDS pAc-sgRNA-Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10632)
AUTHORS Bassett AR, Tibbit C, Ponting CP, Liu JL
TITLE Mutagenesis and homologous recombination in Drosophila cell lines
using CRISPR/Cas9.
JOURNAL Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi:
10.1242/bio.20137120.
PUBMED 24326186
REFERENCE 2 (bases 1 to 10632)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 12, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10632
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_RNA complement(167..242)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter complement(261..660)
/label=dU6-2 promoter
/note="RNA polymerase III promoter for Drosophila U6-2
snRNA (Das et al., 1987)"
CDS 3136..3201
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 3208..3228
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3253..7353
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 7354..7401
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 7417..7470
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 7495..8094
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7495..7514)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7991..8011
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 8102..8236
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8126..8145)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8180..8199
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin complement(8837..9425)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8917..8936)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9596..10456)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10219..10238
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10457..10561)
/gene="bla"
/label=AmpR promoter
primer_bind 10629..15
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ggcgtatcac gaggcccttt cgtcttcaag aattaattct atattctaaa aacacaaatg
61 atacttctaa aaaaaaatca tgaatggcat caactctgaa tcaaatcttt gcagatgcac
121 ctacttctca tttccactgt cacatcattt ttccagatct aaaaaagcac cgactcggtg
181 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
241 acggaagagc tcgctcttcc gaagtattga ggaaaacata cctatataaa tgatcaacat
301 caggaaagag cagttgagaa ttataagaat tggcaaatgg tcttaagaac cctctgctta
361 agattttcaa aatttccttt agaatcaaag tgtcctattg ttcgtttgtt gaaaacacag
421 ttcgatttat tgactataat aaattgatag ttttaaatat agaggcacga ctaagagagt
481 ttggttttgt tttggttagg gaccaaaaaa agtatacata acgaataaaa aaggatttaa
541 gtgcaaatgg taaaaaagtg tcgagttttt cttgagttga ttgtgctgta atgagactct
601 gcattcggca tttgactcgg cttttcctac tcgtgccgta tttcaggctg caagtcgaac
661 agatctcgct gcctgttatg tggcccacaa accaagacac gttttatggc cattaaagct
721 ggctgatcgt cgccaaacac caaatacata atgaatatgt acacattcga gaaagaagcg
781 atcaaagaag cgtcttcggg cggagtagga gaatgcggag gagaaggaga acgagctgat
841 ctagtatctc tccacaatcc aatgccaact gaccaactgg ccatattcgg agcaatttga
901 agccaatttc catcgcctgg cgatcgctcc attcttggct atatgttttt caccgttacc
961 cggggccatt ttcaaagact cgtcggcaag ataagattgt gtcactcgct gtctctcttc
1021 atttgtcgaa gaatgctgag gaatttcgcg atgacgtcgg cgagtatttt gaagaatgag
1081 aataatttgt atttatacga aaatcagtta gtggaatttt ctacaaaaac atgttatcta
1141 tagataattt tgttgcaaaa tatgttgact atgacaaaga ttgtatgtat atctttaatg
1201 tattctcatt ttcttatgta tttataatgg caatgatgat actgatgata ttttaagatg
1261 atgccagacc aaaaggcttg aatttctgcg tcttttgccg aacgcagtgc atgtgcaatt
1321 gttgtttttt ggaatattca attttcggac tgtccgcttt gatttcagtt tcttggctta
1381 ttcaaaaagc aaagtaaagc caaaaaagcg agatggcaat accaaatgcg gcaaaacggt
1441 agtggaagga aaggggtgcg gggcagcgga aggaagggtg gggcggggcg tggcggggtc
1501 tgtggctggg cgcgacgtca ccgacgttgg agccactcct ttgaccatgt gtgcgtgtgt
1561 gtattattcg tgtctcgcca ctcgccggtt gtttttttct ttttatgctg cgctctctct
1621 agcgccatct cgcttacgca tgctcaacgc accgcatgtt gccgtttcct tttatgcgtc
1681 attttggctc gaaataggca attatttaaa caaagattag tcaacgaaaa cgctaaaata
1741 aataagtcta caatatggtt acttattgcc atgtgtgtgc agccaacgat agcaacaaaa
1801 gcaacaacac aggtggcttt ccctctttca ctttttgttt gcaagccgcg tgcgagcaag
1861 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggcgaaaaa
1921 catcaaggcg cctgatacga atgcatttgc aataacaatt gcgatattta atattgttta
1981 tgaagctgtt tgacttcaaa acacacaaaa aaaaaaataa aacaaattat ttgaaagaga
2041 attaggaatc ggacgcttat cgttagggta acaacaagaa atgcttactg agtcacagcc
2101 tctggaaaac tgccgcaagc cagagagaga gagaaaaaga gggagagcag cttagaccgc
2161 atgtgcttgt gtgtgaggcg tctctctctt cgtctctgtt gcgcaaacgc atagactgca
2221 ctgaaaaaat cgattaccta ttttttatga atgaatattt gcactattac tattcaaaac
2281 tattaagata gcaatcacat tcaatagcca aatactatac cacctgagcg atgcaacgaa
2341 atgatcaatt tgagcaaaaa tgctgcatat ttaggacggc atcattatag aaatgcttct
2401 tgctgtgtac ttttctctcg tctggcagct gtttcgccgt tattgttaaa accggcttaa
2461 gttaggtgtg ttttctacga ctagtgaatg ccctactaga agatgtgtgt tgcacaaaat
2521 gtccctggaa taaccaattt gaagtgcaga tagcagtaaa cgtaagctaa tatgaatatt
2581 atttaactgt aatgttttaa tatcgctgga cattactaat aaacccacta taaacacatg
2641 tacatatgta tgttttggca tacaatgagt agttggggaa aaaatgtgta aaagcaccgt
2701 gaccatcaca gcataaagat aaccagctga agtatcgaat atgagtaacc cccaaattga
2761 atcacatgcc gcaactgata ggacccatgg aagtacactc ttcatggcga tatacaagac
2821 acacacaagc acgaacaccc agttgcggag gaaattctcc gtaaatgaaa acccaatcgg
2881 cgaacaattc atacccatat atggtaaaag ttttgaacgc gacttgagag cggagagcat
2941 tgcggctgat aaggttttag cgctaagcgg gctttataaa acgggctgcg ggaccagttt
3001 tcatatcact accgtttgag ttcttgtgct gtgtggatac tcctcccgac acaaagccgc
3061 tccatcagcc agcagtcgtc taatccagag accccggatc ggggtaccta ctagtccagt
3121 gtggtggaat tcatggacta taaggaccac gacggagact acaaggatca tgatattgat
3181 tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga
3241 gtcccagcag ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc
3301 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
3361 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
3421 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
3481 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
3541 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
3601 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
3661 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
3721 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
3781 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
3841 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
3901 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
3961 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
4021 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
4081 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
4141 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
4201 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
4261 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
4321 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
4381 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
4441 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
4501 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
4561 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
4621 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
4681 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
4741 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
4801 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
4861 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
4921 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
4981 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
5041 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
5101 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
5161 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
5221 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
5281 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
5341 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
5401 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
5461 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
5521 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
5581 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
5641 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
5701 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
5761 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
5821 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
5881 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
5941 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
6001 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
6061 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
6121 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
6181 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
6241 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
6301 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
6361 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
6421 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
6481 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
6541 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
6601 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
6661 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
6721 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
6781 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
6841 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
6901 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
6961 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
7021 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
7081 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
7141 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
7201 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
7261 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
7321 acacggatcg acctgtctca gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc
7381 ggccaggcaa aaaagaaaaa gggatcaggc aagcttgagg gcagaggaag tcttctaaca
7441 tgcggtgacg tggaggagaa tcccggccct gctagcggta gcggcagcgg tagcatgacc
7501 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
7561 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
7621 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
7681 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
7741 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
7801 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
7861 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
7921 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
7981 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
8041 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactcgag
8101 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
8161 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
8221 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
8281 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
8341 tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
8401 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
8461 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
8521 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
8581 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
8641 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
8701 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
8761 tagatgacga ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg
8821 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
8881 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
8941 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
9001 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
9061 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
9121 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
9181 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
9241 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
9301 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
9361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
9421 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
9481 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
9541 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
9601 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
9661 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
9721 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
9781 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
9841 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
9901 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
9961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
10021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
10081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
10141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
10201 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
10261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
10321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
10381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
10441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
10501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
10561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
10621 cctataaaaa ta
//
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