pQCasTns(Vch)-entry(BsaI)

LOCUS       pQCasTns(Vch)-entry(BsaI)               11364 bp ds-DNA     circular SYN 22-NOV-2023
DEFINITION  Expression of CRISPR-associated transposases.
ACCESSION   .
VERSION     .
KEYWORDS    pQCasTns(Vch)-entry(BsaI)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S
  TITLE     Orthogonal CRISPR-associated transposases for parallel and 
            multiplexed chromosomal integration.
  JOURNAL   Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi: 
            10.1093/nar/gkab752.
  PUBMED    34478496
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        19..74
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    25..43
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             7111..7122
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8549..8567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8563..8610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8781..9572)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(9573..9664)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(10741..11364)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     11272..11291
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cattaattcc taatttttgt tgacactcta tcattgatag agttatttta ccactcccta
       61 tcagtgatag agaaaagtga actctagaaa taattttgtt taactttaaa aggagatata
      121 ccatgggtga actgccgagt aggtagctga taacgagacc tctggtctcg tgaactgccg
      181 agtaggtagc tgataacgga tccgaattcg agctcggcgc gcctgcaggt cgacaagctt
      241 gcggccgcta aggagatata catatgtttt tgcaaagacc taaaccttac agcgatgaaa
      301 gtttagaaag tttctttatc cgagtggcta acaaaaatgg ctacggtgat gtccatcgct
      361 tcctagaagc cactaaacga ttccttcaag acattgacca taatggctat caaacctttc
      421 cgactgatat aactcggata aacccatact cagctaaaaa cagttccagc gcacgaactg
      481 cgtcattcct gaaacttgca caattgacat ttaatgaacc gccagagcta cttgggttgg
      541 caattaacag aacaaacatg aaatactcgc cgtcaactag cgcggttgtt agaggtgcag
      601 aagtctttcc tcgcagttta ctacggacgc actccatccc ctgctgtcct ttgtgtctgc
      661 gagaaaatgg ctacgcctcc tacctttggc actttcaggg gtacgaatac tgccacagcc
      721 ataacgtacc tttaattacc acttgtagct gtggtaagga gtttgactac cgagtatctg
      781 ggttaaaggg catttgctgc aaatgcaagg agcctatcac cttaaccagc agggagaacg
      841 gtcatgaggc agcgtgtact gtttcaaact ggcttgctgg ccatgaatct aaacctctgc
      901 caaatcttcc taaaagctac cgatggggtt tagttcattg gtggatgggt attaaagata
      961 gcgagttcga tcacttttcg ttcgttcaat ttttctcaaa ctggccaagg tcattccact
     1021 cgataatcga agatgaagta gagttcaacc ttgagcatgc tgttgtcagc acgtctgaat
     1081 tacgactaaa agatcttctt ggtcgattgt ttttcggttc aattcggtta cctgagcgga
     1141 atcttcaaca caatatcatc cttggtgagc ttctctgcta tttagaaaat cgtttatggc
     1201 aagacaaggg attaatcgcc aacctcaaaa tgaacgcgtt agaggcgact gtaatgttaa
     1261 attgtagcct cgatcagatt gcatcaatgg ttgaacaacg catcttgaag ccaaatcgaa
     1321 aaagcaagcc caacagccct cttgatgtta ccgattatct atttcatttc ggcgatattt
     1381 tctgtctttg gttagctgag ttccaaagcg atgagtttaa ccgttcgttt tatgtgtcga
     1441 ggtggtaaat gcaaactctg aaagaactaa tcgcatccaa tcctgacgac ttaacaactg
     1501 aacttaagag agcatttcgt ccactcacac cgcatattgc aattgatggt aatgaacttg
     1561 acgcactgac gatattagtc aatttaaccg ataagactga tgatcagaaa gacctgctcg
     1621 atcgagccaa atgcaagcaa aaacttcgag atgaaaaatg gtgggctagc tgcataaatt
     1681 gcgttaatta cagacaaagc cataacccaa aattcccgga tatacgttct gaaggcgtga
     1741 ttcgaaccca agccctgggt gaattaccga gtttcctact ctcgtcctca aaaatcccac
     1801 cataccattg gtcatatagc catgattcaa aatacgtcaa caaaagcgca ttcctcacca
     1861 atgagttttg ttgggatggt gagatctcat gtttgggtga gcttcttaaa gatgcagatc
     1921 acccactttg gaatactttg aaaaagttag gttgttctca aaaaacttgc aaagcaatgg
     1981 caaaacaact agctgatatt actctcacga ctatcaatgt cacactcgca ccaaattacc
     2041 tgactcaaat atctctcccc gatagtgata catcctacat ttcactctct cctgttgcat
     2101 cgctatcgat gcaaagccac tttcatcaga ggctccaaga tgaaaatcgg catagcgcga
     2161 taacgcggtt tagccgaact accaacatgg gggtcactgc gatgacatgt ggcggcgcat
     2221 ttagaatgtt aaagtctggc gctaagtttt ccagcccccc tcatcaccga ttaaacagta
     2281 aacgaagttg gttgacgtca gagcatgttc agtcattaaa acagtaccag cgcctcaaca
     2341 aaagcctcat acctgaaaac tctcggattg cactccgtag aaaatacaaa atcgagcttc
     2401 aaaatatggt cagatcttgg tttgcaatgc aagaccatac ccttgattcg aacatactta
     2461 tccaacattt gaatcatgac ctatcttact taggagccac aaaacgtttt gcatacgatc
     2521 cagcgatgac caagctcttt actgagcttt tgaaacgaga gttatcaaat tcaatcaata
     2581 atggtgagca acacactaat ggatcgtttt tagtcctacc gaatatcagg gtttgtggcg
     2641 caacagcttt aagctccccg gtaacggtgg ggattccatc acttacagct ttctttggct
     2701 tcgttcacgc atttgaacgg aatataaatc gcaccacctc atcgtttcgt gttgaatcct
     2761 ttgcgatatg cgtccatcaa ctacatgtcg agaagcgagg tttgacagca gagtttgtgg
     2821 aaaaaggcga cgggactata tccgctcccg cgacccggga tgactggcag tgtgatgtcg
     2881 tatttagcct tattttgaac accaactttg ctcaacatat tgaccaagat acgttagtta
     2941 catcactacc aaagcgattg gctcggggtt cagcaaaaat tgcgattgat gactttaaac
     3001 atatcaactc attctcgaca ttagaaacag cgatcgaatc tctgccaata gaagctggta
     3061 ggtggttatc actttacgca cagtcaaaca ataatctaag tgatctatta gcagccatga
     3121 cagaggacca tcagctcatg gcaagctgcg tcggttacca cttgttagaa gagcccaaag
     3181 ataaaccaaa ctccctcaga ggttacaaac acgctatcgc cgagtgcatc attggactca
     3241 ttaactcaat cacctttagc tcagagactg atcccaacac aatcttttgg tcgctaaaga
     3301 actatcaaaa ctacctagtg gtacagccaa ggagtatcaa cgatgaaact accgacaaat
     3361 ctagcctatg agcgctctat cgacccatca gatgtctgtt tttttgtcgt ctggcccgat
     3421 gatagaaaaa cacctttaac ctacaattct cgtactctgc tcgggcaaat ggaagcggca
     3481 tcattagcct atgatgtctc aggtcaacca ataaaaagtg ccaccgctga ggcgttagct
     3541 caagggaacc ctcatcaagt tgatttctgc cacgttccat acggtgcgag tcatattgaa
     3601 tgcagtttct ccgtctcgtt ttcttctgaa ctacgtcaac catataagtg taactcaagc
     3661 aaagttaaac aaacgctagt gcaattagtc gagctctacg aaacgaaaat cggctggact
     3721 gagctagcaa cccgatattt gatgaacatt tgcaacggta aatggctgtg gaaaaatacc
     3781 cgtaaagcct attgctggaa cattgtactt acaccttggc cttggaacgg ggaaaaggtt
     3841 ggatttgaag atattcgtac taactacacc tcacggcaag actttaaaaa taataaaaat
     3901 tggtctgcta tagttgaaat gatcaaaacc gcattttcta gtactgatgg gctggcgata
     3961 tttgaagtca gggccacctt gcacttgcca acgaatgcta tggtgcggcc aagccaagtt
     4021 ttcacagaaa aagaaagtgg cagtaaaagt aaatctaaaa ctcaaaacag tcgagttttt
     4081 cagagtacaa ctattgatgg tgaacgatcg ccaatactag gggcctttaa aacgggagca
     4141 gctattgcaa ccattgacga ctggtatcct gaagccactg agccactaag ggtcggacgg
     4201 tttggggttc atcgcgaaga tgtcacttgc taccgtcatc cgtctaccgg aaaagatttt
     4261 ttctcgatat tacaacaagc agagcactat attgaagtgt tgagcgccaa caaaactccc
     4321 gctcaagaaa ctatcaacga catgcacttt ttaatggcta acctgattaa gggtgggatg
     4381 ttccagcata aaggagactg actgtgaaat ggtattataa gacaatcacc tttctgccag
     4441 agttgtgcaa caacgagtca ctggctgcaa agtgtctccg cgttctgcat ggatttaact
     4501 atcagtatga gacacgaaat atcggcgttt catttccgct ttggtgtgat gcaacggttg
     4561 gaaaaaagat ttcatttgtc agcaagaaca agatagaact cgacttacta cttaaacaac
     4621 actatttcgt ccaaatggaa caacttcaat actttcatat atccaacact gttctcgtcc
     4681 cagaagattg tacatacgtt tcctttagac gctgtcaatc tatagataag ctcacagcag
     4741 cagggctggc aaggaaaatc agacgcctgg agaaacgtgc tctatctaga ggcgagcaat
     4801 ttgacccatc atcttttgct caaaaagagc atactgcaat agcgcactac cactcacttg
     4861 gggagtccag caaacagacg aaccgcaact ttcgactcaa tatcaggatg ctctcggagc
     4921 aaccccgtga ggggaactcg atttttagta gctatggctt atcaaattca gaaaactcgt
     4981 ttcagcctgt acccttaatc tgacctaggc tgcttttaaa aggagatata gcatggcgac
     5041 aagtttacct acgccctcag caattacgac ttcggcgtta gagtatgcat tccatactcc
     5101 cgctcgcaat ctaacgaaat ctcgcggaaa aaacattcat cgttatgtca gtgtaaagat
     5161 gagtaagagg attacggtag aatctactct agagtgtgat gcctgctatc actttgattt
     5221 tgagccaagt attgttcgct tttgcgctca accgattcga tttttatatt atctcaatgg
     5281 tcagtctcac tcctatgttc ctgactttct agttcaattt gataccaacg agtttgttct
     5341 atatgaagta aagtcagctt atgctaagaa caaacctgat tttgatgttg aatgggaggc
     5401 gaaagtaaaa gcagcaactg aactagggct agaattggag cttgttgaag agagtgatat
     5461 tagggatacg gttgtattaa ataatcttaa gcgcatgcat cgttatgctt cgaaagatga
     5521 gctgaataac gtacataact ctctcttaaa aataataaag tacaatggcg cccaatctgc
     5581 aagatgcttg ggagaacagt tgggtttaaa aggccgaact gttttaccaa ttttgtgcga
     5641 tttgctgtca aggtgtttac tcgatacacg tttggataag cctctatctc ttgaatctcg
     5701 atttgagttg gccagttatg gctaagaaag ggttctcaag tttccataga aaagcagtct
     5761 cttcccaaga tacgcttgaa tcgatagagc ttgtctctag cgctaattgt ctagaaagtg
     5821 ttacgtatca agatatatca gcatttcccg aaacaattgc ggtagagatt aatttccgat
     5881 taagcattct tcgcttttta gcgcgaaagt gcgaaaccat tgtggccaaa tcaattgaac
     5941 cacatcgtgt agagctacag caaaactata gtagaaaaat acccagtgca ataacgatat
     6001 atcgatggtg gcttgctttt cgaaaatcag actacaaccc cattagctta gcacctaata
     6061 tcaaggatag aggtaataga gagacaaaag tgtcaacagt tgttgattct attatggaac
     6121 aggcagttga aagagttata tctggacgaa aagtcaatgt tagctctgca tataaacgtg
     6181 ttcgacgaaa agttcgtcaa tacaatctaa ctcatggaac gaaatacacg tatcctaagt
     6241 acgaatctgt aagaaagcga gtaaaaaaga aaaccccatt tgagttatta gccgcaggga
     6301 aaggggagag agtagctaag agagagtttc gccgaatggg aaaaaagatc ctcacgtcta
     6361 gcgtgctaga gagggttgaa atagatcaca ctgtcgttga cctttttgca gtacacgaag
     6421 agtatcgaat cccattgggc cgaccttggc ttactcaatt ggttgattgt tacagtaaag
     6481 ctgtaatcgg tttttattta ggtttcgagc ctcctagcta tgtgtcggtt tcccttgcac
     6541 ttaagaatgc aatacaacgc aaagatgact taatctcctc gtatgaatcg atcgagaatg
     6601 aatggctatg ttatggcatc ccagacctac tcgtaactga taatggtaaa gagtttttgt
     6661 cgaaagcatt tgatcaagca tgtgaatcac tattgatcaa tgtgcatcaa aataaagttg
     6721 agacgcccga caacaaacct catgttgaac gtaactacgg gactattaat acttctctgt
     6781 tagacgattt acctgggaaa tccttcagcc agtaccttca aagagaaggg tacgactctg
     6841 tgggagaagc tacccttaca ctcaatgaga ttagagaaat ttacttaatt tggttggtgg
     6901 atatttatca taaaaaaccc aatcagagag gcactaattg tcctaatgtt gcgtggaaaa
     6961 agggttgtca agaatgggaa ccagaggagt tctctggttc taaagacgaa ttagacttta
     7021 aatttgctat tgttgattac aaacaactta ctaaagtagg gataactgtc tacaaagaac
     7081 tgagttatag caatgaccgt ttagctgaat atagagggaa gaaaggaaac cataaagttc
     7141 agttcaagta taaccctgag tgtatggcag ttatttgggt gttggatgag gatatgaatg
     7201 agtactttac agttaatgcg attgactacg aatatgcaag tagagtatca ctttggcaac
     7261 ataaatataa catgaaatat caagcagaac taaattcagc agaatatgat gaggacaagg
     7321 aaattgatgc agaaataaaa attgaagaaa tcgcagatcg ttcaattgtt aagactaaca
     7381 aaatcagagc tcggaggcgt ggcgctaggc atcaagagaa tagcgcaagg gctaagtcaa
     7441 tcagtaatgc gaacccggcc tcgatacaaa aacatgaaga tgaaatcgtt agtgcagata
     7501 atgacgattg ggatattgat tatgtctgag aaatcgtcaa atgagtgaaa cgcgtgaggc
     7561 tcgtatatca agagctaaaa gggcatttgt atccacaccg agcgttagga aaatcttgag
     7621 ttacatggat agatgtagag atctatcaga cctagagtct gagcctacat gcatgatggt
     7681 ctatggtgct tcgggtgtag gtaaaacgac cgtcatcaag aaatacttaa atcaaaacag
     7741 aagagagtcc gaagccgggg gcgatataat accggttttg catattgagt tgccagacaa
     7801 tgcgaagcca gtagatgcag caagggaatt gctggttgaa atgggtgacc cgctagcact
     7861 ttatgaaact gacttagcta gattgacgaa aagactgact gaattaatcc ctgcggtcgg
     7921 cgtgaagctg attattatcg atgagttcca acatttggtg gaagaaaggt caaatcgggt
     7981 tcttacccaa gtaggtaatt ggctaaaaat gatacttaac aaaacgaaat gtccaattgt
     8041 tatatttggt atgccatact caaaagttgt actgcaagca aactcgcaac ttcacgggcg
     8101 attttccatt caggttgaac tgcgcccctt tagctaccag ggaggtagag gtgtatttaa
     8161 aacttttttg gaataccttg ataaagccct accttttgaa aaacaggctg gcttagccaa
     8221 cgaaagtttg cagaaaaaat tgtatgcatt ctctcaggga aacatgcgtt cgttgagaaa
     8281 ccttatttat caagcatcta tcgaagcaat tgataatcag catgagacga taaccgaaga
     8341 agatttcgtt tttgcatcga agttgacatc gggcgataaa cccaactcat ggaaaaatcc
     8401 ttttgaggag ggtgttgagg taacagaaga tatgttacga ccgccaccaa aagatattgg
     8461 ttgggaggac tatttgagac attcaacccc gagagtgagt aaaccaggta gaaataaaaa
     8521 ctttttcgaa taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     8581 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
     8641 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     8701 taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg
     8761 cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     8821 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
     8881 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     8941 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     9001 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     9061 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     9121 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     9181 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     9241 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     9301 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     9361 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     9421 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     9481 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     9541 gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata ttattgaagc
     9601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9661 caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
     9721 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
     9781 acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     9841 taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     9901 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc
     9961 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta
    10021 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
    10081 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
    10141 cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc
    10201 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
    10261 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
    10321 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
    10381 ggtggttttt tcgaaggtta agtcagttgg ggaactgctt aaccttcgat caaaccacct
    10441 ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa
    10501 gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca
    10561 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
    10621 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ctgatgaatc
    10681 ccctaatgat ttttatcaaa atcattaagg ttaccatcac ggaaaaaggt tatgctgctt
    10741 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
    10801 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
    10861 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
    10921 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
    10981 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
    11041 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
    11101 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
    11161 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
    11221 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
    11281 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
    11341 tttactttta tctaatctag acat
//