| 质粒名称: | pLminP_Luc2P_RE4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90338 |
| 存储实验室: | Ramnik Xavier |
|
载体骨架基本信息 |
|
| 载体名称: | Thermo Fisher |
| 载体出品公司: | Thermo Fisher |
| 空载体大小: | 7251bp |
| 完整质粒大小: | 9293bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Firefly luciferase、Luc2P |
| 物种来源: | P. pyralis |
| 插入大小: | 1776bp |
| 启动子: | NRF2 |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | GGTACAGTGCAGGGGAAAGA |
|
文献引用方法 |
|
| 材料和方法部分: | pLminP_Luc2P_RE4 was a gift from Ramnik Xavier (Addgene plasmid # 90338 ; http://n2t.net/addgene:90338 ; RRID:Addgene_90338) |
| 参考文献部分: | Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre IJ, Mikkelsen TS, Xavier RJ. Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011. Epub 2016 May 19. 10.1016/j.cels.2016.04.011 PubMed 27211859 |
买家导航
pLminP_Luc2P_RE4载体质粒基本信息
pLminP_Luc2P_RE4质粒图谱载体图谱和pLminP_Luc2P_RE4载体序列质粒序列多克隆位点信息
pLminP_Luc2P_RE4质粒载体简介
pLminP_Luc2P_RE4质粒序列载体序列
LOCUS pLminP_Luc2P_RE4 9240 bp DNA circular SYN 15-DEC-2025
DEFINITION NRF2 - Antioxidant response element gene reporter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9240)
AUTHORS O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre
IJ, Mikkelsen TS, Xavier RJ
TITLE Simultaneous Pathway Activity Inference and Gene Expression Analysis
Using RNA Sequencing.
JOURNAL Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
Epub 2016 May 19.
PUBMED 27211859
REFERENCE 2 (bases 1 to 9240)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date:
"2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT Sequence Label: pLminP_Luc2P_RE4
FEATURES Location/Qualifiers
source 1..9240
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1804..1921
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2072..2103
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 2130..2139
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2136..3785
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 3789..3908
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/label=hPEST
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
misc_feature 4000..4588
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4053..4073)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4471..4482)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4603..4919
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4770..4905
/label=SV40 ori
/note="SV40 origin of replication"
CDS join(4970..4972,4973..4975,4976..5689)
/codon_start=1
/product="Emerald GFP"
/label=EmGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5015..5036)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5276..5295)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5623..5644
/label=EGFP-C
/note="EGFP, forward primer"
LTR 5761..5994
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6066..6187
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6103..6122)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6157..6176
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6227..6362
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6382..6401)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6383..6401)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6411..6428)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6411..6427)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6420..6442)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6569..7024
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6656..6675)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6866..6887
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7050..7154
/gene="bla"
/label=AmpR promoter
CDS join(7155..7223,7224..8015)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7373..7392)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8186..8774
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8675..8694
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8928..8945
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 9062..9083
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(9098..9103,9104..9121,9122..9128)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9136..9152
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9141..9163
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9160..9176
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9160..9176
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9195..9215
/label=T3
/note="T3 promoter, forward primer"
promoter 9197..9215
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
1921 tatcgatcgc accagcgtgt ggatccccgt gacaaagcac ccgtgacaaa gcacccgtga
1981 caaagcaccc gtgacaaagc acccgtgaca aagcacccgt gacaaagcag atccgagaac
2041 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
2101 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
2161 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
2221 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
2281 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
2341 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
2401 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
2461 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
2521 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
2581 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
2641 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
2701 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
2761 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
2821 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
2881 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
2941 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
3001 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
3061 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
3121 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
3181 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
3241 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
3301 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
3361 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
3421 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
3481 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
3541 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
3601 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
3661 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
3721 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
3781 ccgtgaattc tcacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
3841 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
3901 tcaacgtcta aggccgcgac tctagaacgc gtcactgcgg ctttgtgtat tctatgctcg
3961 actacagaca tcagcgcgta ccggttagta atgatcgaca atcaacctct ggattacaaa
4021 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
4081 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
4141 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
4201 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
4261 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
4321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
4381 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
4441 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
4501 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
4561 cggatctccc tttgggccgc ctccccgcct ggcgatggta ccggtgtgga aagtccccag
4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
4681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
4741 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
4801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
4861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
4921 agctcccggg agcttgtata tccattttcg gatctgatca gcacctagca tggtgagcaa
4981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
5041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
5101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
5161 cttcacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
5221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
5281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
5341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
5401 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
5461 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
5521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
5581 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
5641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gtacctttaa
5701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
5761 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
5881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
5941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
6001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
6061 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
6181 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
6241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
6301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
6361 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
6421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
6481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
6541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
6601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
6661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
6721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
6781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
6841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
6901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
6961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
7021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
7081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
7141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
7201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
7261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
7321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
7381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
7441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
7501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
7561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
7621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
7681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
7741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
7801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
7861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
7921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
7981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
8041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
8101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
8161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
8221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
8281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
8341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
8401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
8461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
8521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
8581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
8641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
8701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
8761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
8821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
8881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
8941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
9001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
9061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
9121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
9181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
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