| 出品公司: | Novangen |
|---|---|
| 载体名称: | pET-23(+), pET23 |
| 质粒类型: | 大肠杆菌表达载体；蛋白纯化 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 多克隆位点；限制性内切酶 |
| 启动子: | T7/ lacI |
| 载体大小: | 3592 bp |
| 5' 测序引物及序列: | T7 Fwd: 5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | His Tag （C-端） |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 克隆菌株: | DH5a |
| 表达菌株: | BL21系列 |
| 备注: | pET-23a，b，c仅阅读框（读码框）不同，彼此相差1个bp。 |
| 产品目录号: | 71553-3 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型（IPTG) |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET23载体基本信息
pET23载体图谱质粒图谱和多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_promoter | 245 | 227 | |
| ROP | 754 | 945 | |
| pGEX_3_primer | 961 | 939 | |
| pBR322_origin | 1979 | 1360 | |
| Ampicillin | 2994 | 2134 | |
| AmpR_promoter | 3064 | 3036 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 2994 | 2134 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| BglII | 260 |
| MscI | 285 |
| PvuII | 905 |
| PstI | 2452 |
pET23载体简介
pET-23系列载体是一种转录载体，设计用于从克隆插入位点中携带的细菌翻译信号表达。因此，它缺乏存在于pET翻译载体上的核糖体结合位点和ATG起始密码子。pET-23系列载体具有C端T7His标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET23载体序列
LOCUS pET-23(+) 3592 bp DNA SYN
DEFINITION pET-23(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3592
/organism="pET-23(+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
promoter complement(227..245)
/label="T7_promoter"
misc_feature 754..945
/label="ROP"
misc_feature complement(939..961)
/label="pGEX_3_primer"
rep_origin complement(1360..1979)
/label="pBR322_origin"
gene complement(2134..2994)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2134..2994)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(3036..3064)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcctagaggg aaaccgttgt ggtctcccta tagtgagtcg
241 tattaatttc gcgggatcga gatctcgggc agcgttgggt cctggccacg ggtgcgcatg
301 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
361 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
421 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
481 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
541 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
601 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
661 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
721 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
781 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
841 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
901 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
961 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
1021 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
1081 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat
1141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3121 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
3181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
3241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
3301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
3361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
3421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
3481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
3541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca
//
pET23载体图谱质粒图谱pdf版和相关资料下载
pET23载体质粒应用举例
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