pET-43.1c(+)

价格:800元

联系方式:I47-825O-882O

买家导航

pET43.1c载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET43.1c, pET 43.1c
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7230 bp
5' 测序引物: T7
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'
3' 测序引物: ColiDOWN
3' 测序引物序列:   ColiDOWN: 5'-TTCACTTCTGAGTTCGGCATG-3'
载体标签: C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK
载体抗性: Ampicillin
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注:
C term His tag only; N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS
产品目录号: 70941-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET43.1c载体质粒图谱和多克隆位点信息

pet43.1c载体图谱
pet43.1c多克隆位点

Feature Name Start End  
T7_Terminal_primer 69 87
rrnB_T2_terminator 168 141
rrnB_T1_terminator 343 300
rrnB_terminator 466 309
EK 693 679
S15 789 745
6xHIS 816 799
T7_transl_en_RBS 2341 2325
lacO 2386 2359
T7_promoter 2404 2386
tet (300 - 563) 2440 2703
pBRrevBam_primer 2511 2492
lacI 2786 3877
ROP 4449 4640
pGEX_3_primer 4656 4634
pBR322_origin 5674 5055
AmpR_promoter 5761 5789
Ampicillin 5832 6692
ORF Start End  
ORF frame 1 427 2130
ORF frame 1 2316 499
ORF frame 1 2317 3045
ORF frame 2 2918 3877
ORF frame 3 5832 6692
Enzyme Name Cut
PacI 503
XhoI 530
PmlI 576
NotI 592
EagI 592
HindIII 599
KpnI 624
SalI 626
PstI 639
AscI 641
EcoRI 654
BamHI 660
SacI 672
SmaI 719
XmaI 717
SacII 795
SpeI 826
BglII 941
MscI 1073
StuI 1126
NcoI 1401
NdeI 2315
XbaI 2353
ApaI 3356
HpaI 3651
FspI 6396

pET43.1c载体简介

The pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/ expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the ColiDOWN primer (cat. no. 70845-3). Vector encoded sequence can be completely removed when cloning into the PshA I or Sma I sites (as shown below) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively.
pET Vectors 选择指南

pET43.1c载体序列

LOCUS       pET-43.1 c (+)    7230 bp     DNA  Circular   SYN
DEFINITION  pET-43.1 c (+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="pET-43.1 c (+)"
                     /mol_type="other DNA"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     terminator      complement(141..168)
                     /label="rrnB_T2_terminator"
     terminator      complement(300..343)
                     /label="rrnB_T1_terminator"
     terminator      complement(309..466)
                     /label="rrnB_terminator"
     CDS             427..2130
                     /label="ORF frame 1"
     CDS             complement(499..2316)
                     /label="ORF frame 1"
     misc_feature    complement(679..693)
                     /label="EK"
     misc_feature    complement(745..789)
                     /label="S15"
     misc_feature    complement(799..816)
                     /label="6xHIS" 
     CDS             2317..3045
                     /label="ORF frame 1"
     misc_feature    complement(2325..2341)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(2359..2386)
                     /label="lacO" 
     promoter        complement(2386..2404)
                     /label="T7_promoter"
     gene            2440..2703
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(2492..2511)
                     /label="pBRrevBam_primer"
     misc_feature    2786..3877
                     /label="lacI" 
     CDS             2918..3877
                     /label="ORF frame 2"
    misc_feature    4449..4640
                     /label="ROP" 
     misc_feature    complement(4634..4656)
                     /label="pGEX_3_primer"   
     rep_origin      complement(5055..5674)
                     /label="pBR322_origin"  
     promoter        5761..5789
                     /label="AmpR_promoter"  
     gene            5832..6692
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             5832..6692
                     /label="ORF frame 3" 
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca
  121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat
  181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt
  241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
  301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
  361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
  421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc
  481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct
  541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa
  601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg
  661 gatccacgag ctcggactct tgtcgtcgtc atcaatcgta ccagaacccg cgctgccccg
  721 ggggaccaga ccagttggcg gtggcgagtc catgtgctgg cgttcaaatt tcgcagcagc
  781 ggtttcttta cccgcggagt gatggtgatg gtgatgacca gaaccactag tcgcttcgtc
  841 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa
  901 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac
  961 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc
 1021 gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc
 1081 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa
 1141 cagctctttc atcggcacat aggccaattc ttccagcgtc gagaagcctt cttctaccag
 1201 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg
 1261 cgcttccgcc tgatgcttag cttgcaggtc gtcaacggtc atcacgttga gttcccaacc
 1321 gctcagttgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccagatt
 1381 accggcttca acggcgatgt ccatggtgtg tttatcttca tccaccacga tagaagcaac
 1441 gtctgccggt gccattgcgt taatcacgaa ctgcgccggg ttatcatccc acaggacgat
 1501 atcgatacgc tcgccaccca gttcagtaga caccgcctga acacgcgcgc cacgcatacc
 1561 tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg
 1621 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac
 1681 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc
 1741 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa
 1801 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc
 1861 cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg
 1921 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac
 1981 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac
 2041 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt
 2101 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaagtgtcaa aatcaccgct
 2161 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt
 2221 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga
 2281 tacggcttca actacagcca aaatttcttt gttcatatgt atatctcctt cttaaagtta
 2341 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
 2401 attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc
 2461 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
 2521 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
 2581 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
 2641 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
 2701 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
 2761 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
 2821 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
 2881 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
 2941 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
 3001 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
 3061 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
 3121 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
 3181 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
 3241 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
 3301 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
 3361 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
 3421 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
 3481 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
 3541 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc
 3601 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
 3661 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
 3721 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
 3781 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
 3841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
 3901 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
 3961 gctccttccg gtgggcgcgg ggcatgacta gcatgatcgt gctcctgtcg ttgaggaccc
 4021 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
 4081 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
 4141 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
 4201 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
 4261 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
 4321 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
 4381 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
 4441 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
 4501 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
 4561 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
 4621 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
 4681 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
 4741 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
 4801 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc
 4861 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
 4921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
 4981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
 5041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
 5101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
 5161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
 5221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
 5281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
 5341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
 5401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
 5461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
 5521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
 5581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
 5641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
 5701 aacgaaaact cacgttaagg gattttggtc atgacctatt tgtttatttt tctaaataca
 5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat catgattgaa
 5821 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
 5881 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
 5941 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
 6001 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
 6061 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
 6121 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
 6181 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
 6241 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
 6301 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
 6361 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
 6421 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
 6481 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
 6541 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
 6601 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
 6661 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 6721 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 6781 ataatctcat gccatcgtgc cgccagaaat tgtaaacgtt aatattttgt taaaattcgc
 6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
 6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
 6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
 7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
 7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
 7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
 7201 agcggtcacg ctgcgcgtaa ccaccacacc
//

pET43.1c载体质粒图谱pdf版和相关资料下载

pET43.1c载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng