pET-43.1b(+)

价格:800元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

pET43.1b载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET43.1b, pET 43.1b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7274 bp
5' 测序引物: T7
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'
3' 测序引物: ColiDOWN
3' 测序引物序列:   ColiDOWN: 5'-TTCACTTCTGAGTTCGGCATG-3'
载体标签: C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK
载体抗性: Ampicillin
备注:
C term His tag only; N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS
产品目录号: 70940-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒
表达宿主菌: BL21(DE3)BLR(DE3)Rosetta(DE3)OrigamiB(DE3)C43(DE3)
pET表达手册: pET表达手册英文版
pET表达手册中文版
pET载体汇总:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET43.1b载体图谱质粒图谱和多克隆位点信息

pet43.1b载体图谱
pet43.1b多克隆位点

Feature Name Start End  
T7_Terminal_primer 69 87
rrnB_T2_terminator 168 141
rrnB_T1_terminator 343 300
rrnB_terminator 466 309
EK 694 680
S15 790 746
6xHIS 817 800
T7_transl_en_RBS 2342 2326
lacO 2387 2360
T7_promoter 2405 2387
tet (300 - 563) 2441 2704
pBRrevBam_primer 2512 2493
lacI 2787 3878
ROP 4450 4641
pGEX_3_primer 4657 4635
pBR322_origin 5675 5056
AmpR_promoter 5762 5790
Ampicillin 5833 6693
ORF Start End  
ORF frame 2 2317 509
ORF frame 2 803 2131
ORF frame 2 2318 3046
ORF frame 3 2919 3878
ORF frame 1 5833 6693
Enzyme Name Cut
PacI 503
XhoI 530
PmlI 576
NotI 592
EagI 592
HindIII 599
KpnI 624
SalI 626
PstI 639
AscI 641
EcoRI 654
BamHI 660
SacI 672
SmaI 720
XmaI 718
SacII 796
SpeI 827
BglII 942
MscI 1074
StuI 1127
NcoI 1402
NdeI 2316
XbaI 2354
ApaI 3357
HpaI 3652
FspI 6397

pET43.1b载体简介

pET-43.1系列载体是带有Nus蛋白标签,可以在大肠杆菌中高表达目的蛋白。本载体带有N-Trx标签和His标签,S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是,这些载体序列是依据载体pBR322的方向来进行编号的,所以T7标签表达区域在载体图谱上是反向的。

T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的,所以在T7噬菌体聚合酶的作用下,包含有蛋白编码序列的病毒 粒子能够产生,并启动蛋白表达,同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。


pET Vectors 选择指南

pET43.1b载体序列

LOCUS       pET-43.1b (+)    7274 bp     DNA  Circular    SYN
DEFINITION  pET-43.1b (+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="pET-43.1 b (+)"
                     /mol_type="other DNA"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     terminator      complement(141..168)
                     /label="rrnB_T2_terminator"
     terminator      complement(300..343)
                     /label="rrnB_T1_terminator"
     terminator      complement(309..466)
                     /label="rrnB_terminator"
     CDS             complement(509..2317)
                     /label="ORF frame 2"
     misc_feature    complement(680..694)
                     /label="EK"
     misc_feature    complement(746..790)
                     /label="S15" 
     misc_feature    complement(800..817)
                     /label="6xHIS"
     CDS             803..2131
                     /label="ORF frame 2"
     CDS             2318..3046
                     /label="ORF frame 2"
     misc_feature    complement(2326..2342)
                     /label="T7_transl_en_RBS" 
     misc_feature    complement(2360..2387)
                     /label="lacO"      
     promoter        complement(2387..2405)
                     /label="T7_promoter"  
     gene            2441..2704
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(2493..2512)
                     /label="pBRrevBam_primer"     
     misc_feature    2787..3878
                     /label="lacI"  
     CDS             2919..3878
                     /label="ORF frame 3"
     misc_feature    4450..4641
                     /label="ROP"
     misc_feature    complement(4635..4657)
                     /label="pGEX_3_primer"   
     rep_origin      complement(5056..5675)
                     /label="pBR322_origin"
     promoter        5762..5790
                     /label="AmpR_promoter"
     gene            5833..6693
                     /label="Ampicillin"
                     /gene="Ampicillin" 
     CDS             5833..6693
                     /label="ORF frame 1" 
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca
  121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat
  181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt
  241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
  301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
  361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
  421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc
  481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct
  541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa
  601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg
  661 gatccacgag ctccggactc ttgtcgtcgt catcaatcgt accagaaccc gcgctgcccc
  721 gggggaccag accagttggc ggtggcgagt ccatgtgctg gcgttcaaat ttcgcagcag
  781 cggtttcttt acccgcggag tgatggtgat ggtgatgacc agaaccacta gtcgcttcgt
  841 caccgaacca gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca
  901 acccttcgat atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa
  961 cgccacgggc ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat
 1021 cgtcagccgg tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg
 1081 catttttagc acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca
 1141 acagctcttt catcggcaca taggccaatt cttccagcgt cgagaagcct tcttctacca
 1201 gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt
 1261 gcgcttccgc ctgatgctta gcttgcaggt cgtcaacggt catcacgttg agttcccaac
 1321 cgctcagttg cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccagat
 1381 taccggcttc aacggcgatg tccatggtgt gtttatcttc atccaccacg atagaagcaa
 1441 cgtctgccgg tgccattgcg ttaatcacga actgcgccgg gttatcatcc cacaggacga
 1501 tatcgatacg ctcgccaccc agttcagtag acaccgcctg aacacgcgcg ccacgcatac
 1561 ctacgcaagc acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac
 1621 gagaacccgg atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca
 1681 cttcaatacg gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg
 1741 cgccacgcgc ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga
 1801 agttttcacg cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat
 1861 ccagagagat gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt
 1921 gttcacggaa ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa
 1981 cctgttttgc cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa
 2041 cgtaatcgcc caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct
 2101 tggtcggctg ggtgacttca tcaacaacta accagcgacg gaaagtgtca aaatcaccgc
 2161 ttttgcgatc gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg
 2221 tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg
 2281 atacggcttc aactacagcc aaaatttctt tgttcatatg tatatctcct tcttaaagtt
 2341 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
 2401 tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca tcgtggccgg
 2461 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
 2521 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
 2581 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
 2641 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
 2701 agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat
 2761 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
 2821 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
 2881 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
 2941 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
 3001 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
 3061 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
 3121 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
 3181 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
 3241 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
 3301 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
 3361 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
 3421 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
 3481 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
 3541 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggacatct
 3601 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
 3661 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
 3721 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
 3781 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
 3841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
 3901 tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc
 3961 agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc gttgaggacc
 4021 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
 4081 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
 4141 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
 4201 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
 4261 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
 4321 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
 4381 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
 4441 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
 4501 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
 4561 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
 4621 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
 4681 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
 4741 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
 4801 catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg
 4861 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
 4921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 4981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 5041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
 5101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 5161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 5221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 5281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 5341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 5401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 5461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
 5521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 5581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 5641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 5701 gaacgaaaac tcacgttaag ggattttggt catgacctat ttgtttattt ttctaaatac
 5761 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa tcatgattga
 5821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
 5881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
 5941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
 6001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
 6061 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
 6121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
 6181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
 6241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
 6301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
 6361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
 6421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
 6481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
 6541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
 6601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
 6661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
 6721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
 6781 gataatctca tgccatcgtg ccgccagaaa ttgtaaacgt taatattttg ttaaaattcg
 6841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
 6901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
 6961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
 7021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
 7081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
 7141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
 7201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
 7261 cgtcccattc gcca
//

pET43.1b载体图谱质粒图谱pdf版和相关资料下载

pET43.1b载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....