pET-41b(+)

价格:500元

联系方式:I47-825O-882O

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pET41b载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET41b, pET 41b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5932 bp
5' 测序引物: T7或pGEX-5‘
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
载体标签: N-GST, N-His, N-Thrombin
载体抗性: Kanamycin
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注:
Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; PshAI blunt cloning site; a,b,c vary by MCS
产品目录号: 70557-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET41b载体质粒图谱和多克隆位点信息

pet41b载体图谱
pet41b多克隆位点
pet41b载体克隆方法说明

Feature Name Start End  
T7_terminator 120 1
T7_Terminal_primer 69 87
EK 278 264
S15 353 309
6xHIS 413 396
GST (variant) 1094 444
T7_transl_en_RBS 1119 1103
lacO 1164 1137
T7_promoter 1182 1164
tet (300 - 563) 1218 1481
pBRrevBam_primer 1289 1270
lacI 1564 2655
ROP 3227 3418
pGEX_3_primer 3434 3412
pBR322_origin 4452 3833
KanR2 4558 5373
ORF Start End  
ORF frame 3 1094 147
ORF frame 3 1095 1823
ORF frame 1 1696 2655
ORF frame 1 4558 5373
Enzyme Name Cut
XhoI 174
NotI 182
EagI 182
HindIII 189
SalI 195
SacI 206
PstI 214
AscI 216
StuI 226
EcoRI 235
BamHI 241
EcoRV 251
NcoI 254
AgeI 291
KpnI 298
BglII 301
SacII 392
SpeI 423
MscI 886
NdeI 1093
XbaI 1131
ApaI 2134
HpaI 2429
NruI 4646
SmaI 4863
XmaI 4861

pET41b载体简介

The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3).Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.

pET41b载体序列

LOCUS       pET-41 b(+)    5932 bp     DNA Circular    SYN
DEFINITION  pET-41 b(+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="pET-41 b(+)"
                     /mol_type="other DNA"
     terminator      complement(1..120)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(147..1094)
                     /label="ORF frame 3"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
                     KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
     misc_feature    complement(264..278)
                     /label="EK"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
                     KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
     misc_feature    complement(309..353)
                     /label="S15"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
                     KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
     misc_feature    complement(396..413)
                     /label="6xHIS"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
                     KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
     gene            complement(444..1094)
                     /label="GST (variant)"
                     /gene="GST (variant)"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
                     KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
     CDS             1095..1823
                     /label="ORF frame 3"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1103..1119)
                     /label="T7_transl_en_RBS"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1137..1164)
                     /label="lacO"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     promoter        complement(1164..1182)
                     /label="T7_promoter"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     gene            1218..1481
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(1270..1289)
                     /label="pBRrevBam_primer"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    1564..2655
                     /label="lacI"
                     /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
                     IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
                     PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
                     ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
                     TVVADWRCHLQSGPARAVANCRGD*"
     CDS             1696..2655
                     /label="ORF frame 1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    3227..3418
                     /label="ROP"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    complement(3412..3434)
                     /label="pGEX_3_primer"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     rep_origin      complement(3833..4452)
                     /label="pBR322_origin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            4558..5373
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             4558..5373
                     /label="ORF frame 1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
  241 ggatccccga tatccatggg actcttgtcg tcgtcatcac cggagccacc accggtaccc
  301 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca
  361 attgcagtac taccgcgtgg caccagaccc gcggagtgat ggtgatggtg atgaccagaa
  421 ccactagttg aaccatccga ttttggagga tggtcgccac caccaaacgt ggcttgccag
  481 ccctgcaaag gccatgctat atacttgctg gatttcaagt acttatcaat ttgtgggata
  541 gcttcaatac gttttttaaa acaaactaat tttgggaacg catccaggca cattgggtcc
  601 atgtataaaa caacatcaag agcgtcatac aacatgaagt caggatgggt tacatgatca
  661 ccatttaaat atgttttatg acataaacga tcttcgaaca ttttcagcat ttcaggtagc
  721 ttgctaagaa aatcaacttt gagagtttca aagtctttac tatatgcaat tctcgaaaca
  781 ccgtatctaa tatccaaaac cgctccttca agcattgaaa tctctgcacg ctcttttgga
  841 caaccaccca acatgttgtg cttgtcagct atataacgta tgatggccat agactgtgtt
  901 aatttaacat caccatcaat ataataagga agattgggaa actccaaacc caattcaaac
  961 tttttgtttc gccatttatc accttcatcg cgctcataca aatgctcttc atatttttct
 1021 tcaagatatt ccaaaagaag tcgagtgggt tgcacaaggc ccttaatttt ccaataacct
 1081 agtatagggg acatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
 1141 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
 1201 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
 1261 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
 1321 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
 1381 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
 1441 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc
 1501 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
 1561 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
 1621 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
 1681 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
 1741 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
 1801 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
 1861 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
 1921 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
 1981 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
 2041 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
 2101 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
 2161 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
 2221 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
 2281 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
 2341 attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata cgacgatacc
 2401 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
 2461 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
 2521 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
 2581 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
 2641 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg
 2701 atctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
 2761 catgactagc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
 2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
 2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
 2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
 3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
 3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
 3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
 3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
 3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
 3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
 3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
 3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
 3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
 3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
 3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
 3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
 3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
 3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
 3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
 4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
 4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
 4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
 4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
 4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
 4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
 4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
 4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
 4501 ttttggtcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
 4561 agccatattc aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct
 4621 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
 4681 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
 4741 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
 4801 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
 4861 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
 4921 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
 4981 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
 5041 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
 5101 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
 5161 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
 5221 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
 5281 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
 5341 cagtttcatt tgatgctcga tgagtttttc taagaattaa ttcatgagcg gatacatatt
 5401 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
 5461 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
 5521 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
 5581 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
 5641 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
 5701 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
 5761 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
 5821 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
 5881 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca

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