| 出品公司: | Novagen |
|---|---|
| 载体名称: | pET-3d(+) |
| 质粒类型: | 大肠杆菌载体 |
| 克隆菌株: | DH5α |
| 启动子: | T7 |
| 终止子: | T7 terminator |
| 载体大小: | 4637bp |
| 5' 测序引物及序列: | T7:TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | T7-ter:TGCTAGTTATTGCTCAGCGG |
| 载体标签: | N-T7 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α |
| 表达宿主: | BL21(DE3) |
| 培养条件: | 37℃，有氧，LB |
| 诱导方式: | 无需诱导 |
| 组成型: | 组成型 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET3d载体基本信息
pET3d载体图谱质粒图谱和多克隆位点信息
pET3d载体简介
pET-3a-d载体携带一个N端t7•tag序列和BamH I克隆位点。这些 载体是许多pET家族载体的前身；pET-23a-d（+）系列对应于 PET-3A-D，但包含几个附加功能。独特的地点显示在圆形地图上。 注意，序列由pbr322约定编号，因此t7表达式区域是 在图谱上反转。转录的编码链的克隆/表达区 T7 RNA聚合酶如下所示。PET-3A-D载体携带一个N末端T7•Tag®序列和BAMH I克隆位点。这些 载体是许多pet家族载体的前身；pet3d系列对应于 PET-3A-D，但包含几个附加功能。独特的地点显示在圆形地图上。 注意，序列由pbr322约定编号，因此t7表达式区域是 在图谱上反转。
pET3d载体序列
LOCUS pET-3d(+) 4637 bp ds-DNA circular SYN 25-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pET-3d
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4637)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, August 25, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4637
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..19
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 51..73
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 80..112
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
terminator 178..225
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(591..619)
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
promoter 732..836
/gene="bla"
/note="AmpR promoter"
CDS 837..1697
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1868..2456
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2642..2784
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(2886..3077)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
61 tttaagaagg agatatacca tggctagcat gactggtgga cagcaaatgg gtcggatccg
121 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
181 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
241 atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
301 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
361 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
421 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
481 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
541 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
601 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
661 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
841 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
901 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
961 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
1021 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
1081 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
1141 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
1201 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
1261 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
1321 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
1381 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
1441 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
1501 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
1561 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
1621 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
1681 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
1741 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
1801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
1861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
1981 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
2041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
2101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
2161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
2221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
2281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
2401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
2461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2521 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
2581 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2641 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
2701 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
2761 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
2821 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
2881 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
2941 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
3001 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
3061 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
3121 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
3181 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
3241 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
3301 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
3361 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
3421 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
3481 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
3541 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac
3601 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
3661 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
3721 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
3781 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
3841 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
3901 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
3961 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
4021 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
4081 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
4141 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
4201 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
4261 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
4321 ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca
4381 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
4441 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
4501 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
4561 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
4621 tctcgatccc gcgaaat
//
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pET3d载体质粒应用举例
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