pET-32 Xa/LIC

价格:800元

联系方式:I47-825O-882O

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pET32 Xa/LIC载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET32 Xa/LIC, pet 32 Xa/LIC
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5926 bp
5' 测序引物: T7或者Trx-F
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3'; Trx-F: 5' TTCCTCGACGCTAACCTG 3'
载体标签: thioredoxin (N端); His (中间和C端), N- Factor Xa
载体抗性: Ampicillin
表达宿主菌: Origami2(DE3)Origami(DE3)OrigamiB(DE3)BL21-TrxB(DE3)
备注:
For directional cloning of PCR-amplified DNA; ligation independent cloning; Factor Xa cleavage site
产品目录号: 69072-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET32 Xa/LIC载体质粒图谱和多克隆位点信息

pet32 Xa/LIC载体图谱
pet32 Xa/LIC多克隆位点
pet32 Xa/LIC克隆方法

Feature Name Start End  
T7_terminator 129 1
T7_Terminal_primer 69 87
S15 319 275
lacI 328 2279
6xHIS 370 353
T7_transl_en_RBS 743 727
lacO 788 761
T7_promoter 806 788
tet (300 - 563) 842 1105
pBRrevBam_primer 913 894
tet (576 - 851) 2338 2613
ROP 3088 3279
pGEX_3_primer 3295 3273
pBR322_origin 4313 3694
Ampicillin 5328 4468
AmpR_promoter 5398 5370
ORF Start End  
ORF frame 1 718 137
ORF frame 2 353 1447
ORF frame 3 1320 2279
ORF frame 2 5328 4468
Enzyme Name Cut
XhoI 158
NotI 166
EagI 166
HindIII 173
SalI 179
SacI 190
EcoRI 192
BamHI 198
EcoRV 206
NcoI 211
AgeI 257
KpnI 264
BglII 267
BstBI 294
XbaI 755
BclI 1561
ApaI 1758
HpaI 2053
PstI 4786

pET32 Xa/LIC载体简介

The pET-32 Xa/LIC vector is designed for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with the 109aa Trx•Tag™ thioredoxin protein (1), His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-32 Xa/LIC Vector Kit (Cat. No. 69072-3), inserts can be efficiently cloned without the need for restriction digestion or ligation, and the resulting fusion proteins can be cleaved with Factor Xa to precisely remove all vector-encoded amino acids. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

pET32 Xa/LIC载体序列

LOCUS       pET-32 Xa/LIC    5926 bp     DNA  Circular   SYN
DEFINITION  pET-32 Xa/LIC
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="pET-32 Xa/LIC"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     CDS             complement(137..718)
                     /label="ORF frame 1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     misc_feature    complement(275..319)
                     /label="S15"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     misc_feature    328..2279
                     /label="lacI"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
                     GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
     CDS             353..1447
                     /label="ORF frame 2"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(353..370)
                     /label="6xHIS"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(727..743)
                     /label="T7_transl_en_RBS"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(761..788)
                     /label="lacO"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     promoter        complement(788..806)
                     /label="T7_promoter"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     gene            842..1105
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     misc_feature    complement(894..913)
                     /label="pBRrevBam_primer"
                     /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
                     SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
                     RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
                     ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
                     AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
                     IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
                     TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
     CDS             1320..2279
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            2338..2613
                     /label="tet (576 - 851)"
                     /gene="tet (576 - 851)"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    3088..3279
                     /label="ROP"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     misc_feature    complement(3273..3295)
                     /label="pGEX_3_primer"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     rep_origin      complement(3694..4313)
                     /label="pBR322_origin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     gene            complement(4468..5328)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(4468..5328)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5370..5398)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
  181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct
  241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt
  301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat
  361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca
  421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca
  481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
  541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
  601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
  661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
  721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
  781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta
  841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
  901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
  961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa
 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc
 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg
 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg
 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg
 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//

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