pET-30b(+)

价格:800元

联系方式:I47-825O-882O

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pET30b载体基本信息

出品公司: EMD Biosciences (Novagen)
别名: pET30b, pet 30b
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5421 bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-His, N-S, N-Thrombin, N-EK, C-His
载体抗性: Kanamycin
表达宿主菌: BL21(DE3) BLR(DE3)BL21(DE3)pLysS
备注:
N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS
 
产品目录号: 69910-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET30b载体质粒图谱和多克隆位点信息

pet30b载体图谱
pet30b多克隆位点

pET30b载体简介

The pET-30a-c(+) vectors carry an N-terminal His• Tag®/ thrombin/ S• Tag™/ enterokinase configuration plus an optional C-terminal His• Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

pET30b载体序列

LOCUS       pET-30 b (+)    5421 bp     DNA circular    SYN
DEFINITION  pET-30 b (+)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="pET-30 b (+)"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label="T7_terminator"
     misc_feature    69..87
                     /label="T7_Terminal_primer"
     misc_feature    complement(218..229)
                     /label="EK"
     misc_feature    301..1907
                     /label="lacI"
     CDS             326..1075
                     /label="ORF frame 2"
     misc_feature    complement(355..371)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(389..416)
                     /label="lacO"
     promoter        complement(416..434)
                     /label="T7_promoter"
     gene            470..733
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(522..541)
                     /label="pBRrevBam_primer"
     CDS             948..1907
                     /label="ORF frame 3"
     gene            1966..2241
                     /label="tet (576 - 851)"
                     /gene="tet (576 - 851)"
     misc_feature    2716..2907
                     /label="ROP"
     misc_feature    complement(2901..2923)
                     /label="pGEX_3_primer"
     rep_origin      complement(3322..3941)
                     /label="pBR322_origin"
     gene            4047..4862
                     /label="KanR2"
                     /gene="KanR2"
     CDS             4047..4862
                     /label="ORF frame 3"
ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
  121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
  181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca
  241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag
  301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt
  361 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
  421 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat
  481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
  541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
  601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
  661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
  721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta
  781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
  841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
  901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
  961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
 1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
 1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
 1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
 1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
 1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
 1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
 1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
 1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
 1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
 1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
 1621 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
 1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
 1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
 1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
 1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
 1921 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
 1981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
 2041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
 2101 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
 2161 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
 2221 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg
 2281 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
 2341 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
 2401 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
 2461 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
 2521 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
 2581 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
 2641 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
 2701 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
 2761 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
 2821 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
 2881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
 2941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
 3001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
 3061 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca
 3121 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
 3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
 3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
 3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
 3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
 3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
 3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
 3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
 3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
 3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
 3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
 3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
 3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
 3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
 3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt
 4021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta
 4081 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
 4141 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
 4201 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
 4261 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
 4321 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
 4381 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
 4441 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
 4501 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
 4561 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
 4621 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
 4681 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
 4741 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
 4801 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
 4861 aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
 4921 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta
 4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
 5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
 5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
 5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
 5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
 5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
 5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
 5401 cagggcgcgt cccattcgcc a
//

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