pET-23d(+)

价格:800元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pET23d载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET23d, pet 23d
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3663bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-T7, C-His
载体抗性: Ampicillin
备注:
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
产品目录号: 69748-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒
表达宿主菌: BL21(DE3)BLR(DE3)Rosetta(DE3)OrigamiB(DE3)
pET表达手册: pET表达手册英文版
pET表达手册中文版
pET载体汇总:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET23d载体图谱质粒图谱和多克隆位点信息

pet23a载体图谱
pet23a多克隆位点
pet23a载体图谱

pET23d载体简介

pET-23a-d系列载体带有N-T7标签和C-His标签。pET-23a-d系列载体与pET-21a-d系列载体相比,启动子由pet21a系列载体的T7lac启动子,更改为pET-23a-d载体的T7启动子。独特的酶切位点在载体图谱中被标注出来。需要注意的是,这些载体序列是依据载体pBR322的方向来进行编号的,所以T7标签表达区域在载体图谱上是反向的。


pET Vectors 选择指南

pET23d载体序列

LOCUS       pET-23d(+)              3663 bp    DNA     circular     11-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     promoter        complement(300..316)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(205..237)
                     /vntifkey="31"
                     /label=T7\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     rep_origin      complement(1447..1447)
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(2208..3065)
                     /vntifkey="4"
                     /label=bla(amp)\sequence
     rep_origin      complement(3197..3652)
                     /vntifkey="33"
                     /label=f1\origin
BASE COUNT      918 a       935 c       926 g       884 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
      241 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct
      301 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac
      361 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
      421 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
      481 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
      541 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
      601 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
      661 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
      721 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
      781 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
      841 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
      901 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
      961 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     1141 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     1201 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3181 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     3241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     3661 cca
//

pET23d载体图谱质粒图谱pdf版和相关资料下载

pET23d载体质粒应用举例

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