pET-23d(+)

价格:800元

联系方式:I47-825O-882O

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pET23d载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pET23d, pet 23d
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3663bp
5' 测序引物: T7
5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
载体标签: N-T7, C-His
载体抗性: Ampicillin
备注:
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
产品目录号: 69748-3
稳定性: 瞬时表达 Transient
组成型: 组成型 Constitutive
病毒/非病毒: 非病毒

pET23d载体质粒图谱和多克隆位点信息

pet23a载体图谱
pet23a多克隆位点
The maps for pET-23b(+), pET-23c(+) and pET-23d(+) are the same as pET-23a(+) (shown) with the following exceptions: pET-23b(+) is a 3665bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-23c(+) is a 3664bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-23d(+) is a 3663bp plasmid; the BamH I site is in the same reading frame as in pET-23c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-23c(+). As a result, Nco I cuts pET-23d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-23a(+). Nde I does not cut pET-23d(+). Note also that Sty I is not unique in pET-23d(+).

pET23d载体简介

The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) by the “plain” T7 promoter instead of the T7lac promoter and by the absence of the lacI gene. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

pET23d载体序列

LOCUS       pET-23d(+)              3663 bp    DNA     circular     11-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     promoter        complement(300..316)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(205..237)
                     /vntifkey="31"
                     /label=T7\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     rep_origin      complement(1447..1447)
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(2208..3065)
                     /vntifkey="4"
                     /label=bla(amp)\sequence
     rep_origin      complement(3197..3652)
                     /vntifkey="33"
                     /label=f1\origin
BASE COUNT      918 a       935 c       926 g       884 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
      241 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct
      301 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac
      361 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
      421 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
      481 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
      541 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
      601 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
      661 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
      721 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
      781 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
      841 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
      901 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
      961 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     1141 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     1201 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3181 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     3241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     3661 cca
//

pET23d载体质粒图谱pdf版和相关资料下载

pET23d载体应用举例

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