pSL892

LOCUS       pSL892              7595 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             542..1129
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     misc_feature    1136..1879
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1203..1225)
                     /label=186-WKG-603-R
     misc_feature    1409..1411
                     /label=F93A
                     /note="F93A"
     primer_bind     1776..1802
                     /label=206-WKG-655-F
     misc_feature    1880..3151
                     /label=mFGFR 405-end
                     /note="mFGFR 405-end"
     misc_feature    2714..2716
                     /label=Y677
                     /note="Y677"
     polyA_signal    3192..3416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3462..3890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3476..3500)
                     /label=184-WKG-599-R
     primer_bind     3761..3780
                     /label=189-WKG-612-F
     promoter        3904..4233
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4300..5094
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4344..4371)
                     /label=188-WKG-610-R
     polyA_signal    5268..5389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5434..5454)
                     /label=M13-rev
     primer_bind     complement(5438..5454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5460..5482)
                     /label=LacO
     protein_bind    5462..5478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5486..5516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5487..5516)
                     /label=lac
     protein_bind    5531..5552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5658..6598
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6599..7459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7460..7564)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 caagaagaag ggcagcgtgg tgatcgtggg cagaatcaac ctgagcggcg acaccgccta
      601 cagccagcag accagaggcc tggagggctg ccaggagacc agccagaccg gcagagacaa
      661 gaaccaggtg gagggcgagg tgcaggtggt gagcaccgcc acccagagct tcctggccac
      721 cagcatcaac ggcgtgctgt ggaccgtgta ccacggcgcc ggcaccagaa ccatcgccag
      781 ccccaagggc cccgtgaccc agatgtacac gaacgtggac aaggacttag tgggctggca
      841 ggccccccaa ggcagcagaa gcctgacccc ctgcacctgc ggcagcagcg acctgtacct
      901 ggtgaccaga cacgccgacg tgatccccgt gagaagaaga ggcgacagca gaggcagcct
      961 gctgagcccc agacccatca gctatctgaa aggctcaagc ggcggccccc tgttgtgtcc
     1021 cgccggccat gccgtgggca tcttcagagc cgccgtgagc accagaggcg tggccaaggc
     1081 cgtggacttc atccccgtgg agagcctgga gaccaccatg agaagccccg aattcgcacc
     1141 ttcacccagc ctcccggacc ccaagtttga gagcaaagcg gccctgctgg catcccgggg
     1201 ctccgaagaa cttctgtgct tcacccaacg cttggaagac ttggtgtgtt tctgggagga
     1261 agcggcgagc tccgggatgg acttcaacta cagcttctca taccagctcg agggtgagtc
     1321 acgaaagtca tgtagcctgc accaggctcc caccgtccgc ggctccgtgc gtttctggtg
     1381 ttcactgcca acagcggaca catcgagtgc tgtgccgctg gagctgcagg tgacggaggc
     1441 gtccggttct cctcgctatc accgcatcat ccatatcaat gaagtagtgc tcctggacgc
     1501 ccccgcgggg ctgctggcgc gccgggcaga agagggcagc cacgtggtgc tgcgctggct
     1561 gccacctcct ggagcaccta tgaccaccca catccgatat gaagtggacg tgtcggcagg
     1621 caaccgggca ggagggacac aaagggtgga ggtcctggaa ggccgcactg agtgtgttct
     1681 gagcaacctg cggggcggga cgcgctacac cttcgctgtt cgagcgcgca tggccgagcc
     1741 gagcttcagc ggattctgga gtgcctggtc tgagcccgcg tcactactga ccgctagcga
     1801 cctggaccct ctcatcttga cgctgtctct cattctggtc ctcatctcgt tgttgctgac
     1861 ggttctggcc ctgctgtcca tgaagagcgg caccaagaag agcgacttcc atagccagat
     1921 ggctgtgcac aagctggcca agagcatccc tctgcgcaga caggtaacag tgtcagctga
     1981 ctccagtgca tccatgaact ctggggttct cctggttcgg ccctcacggc tctcctccag
     2041 cgggaccccc atgctggctg gagtctccga atatgagctc cctgaggacc cccgctggga
     2101 gctgccacga gacagactgg tcttaggcaa accacttggc gagggctgct tcgggcaggt
     2161 ggtgttggct gaggccatcg ggctggataa ggacaaaccc aaccgtgtga ccaaagtggc
     2221 cgtgaagatg ttgaagtccg acgcaacgga gaaggacctg tcggatctga tctcggagat
     2281 ggagatgatg aaaatgattg ggaagcacaa gaatatcatc aaccttctgg gagcgtgcac
     2341 acaggatggt cctctttatg tcattgtgga gtacgcctcc aaaggcaatc tccgggagta
     2401 tctacaggcc cggaggcctc ctgggctgga gtactgctat aaccccagcc acaaccccga
     2461 ggaacagctg tcttccaaag atctggtatc ctgtgcctat caggtggctc ggggcatgga
     2521 gtatcttgcc tctaagaagt gtatacaccg agacctggct gctaggaacg tcctggtgac
     2581 cgaggataac gtaatgaaga tcgcagactt tggcttagct cgagacattc atcatatcga
     2641 ctactacaag aaaaccacca acggccggct gcctgtgaag tggatggccc ctgaggcgtt
     2701 gtttgaccgg atctacacac accagagcga tgtgtggtct tttggagtgc tcttgtggga
     2761 gatcttcact ctgggtggct ccccataccc cggtgtgcct gtggaggaac ttttcaagct
     2821 gctgaaggag ggtcatcgaa tggacaagcc cagtaactgt accaatgagc tgtacatgat
     2881 gatgcgggac tgctggcatg cagtgccctc tcagagacct acgttcaagc agttggtgga
     2941 agacctggac cgcattgtgg ccttgacctc caaccaggag tatctggacc tgtccatacc
     3001 gctggaccag tactcaccca gctttcccga cacacggagc tccacctgct cctcagggga
     3061 ggactctgtc ttctctcatg agccgttacc tgaggagccc tgtctgcctc gacaccccac
     3121 ccagcttgcc aacagtggac tcaaacggcg ctagtctaga gggcccgttt aaacccgctg
     3181 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3241 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3301 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3361 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3421 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3901 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3961 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4081 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4141 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4201 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4261 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4321 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4381 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4441 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4501 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4561 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4621 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4681 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4741 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4801 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4861 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4921 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4981 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5041 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5101 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5161 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5221 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5281 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5401 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5461 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5521 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5581 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5641 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5701 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5761 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5821 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5881 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5941 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6001 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6061 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6121 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6181 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6241 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6301 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6361 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6421 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6481 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6541 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6601 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6661 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6721 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6781 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6841 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6901 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6961 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7021 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7081 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7141 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7201 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7261 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7321 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7381 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7441 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7501 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7561 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//