| 质粒名称: | pSL582 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000650 |
| 产品用途: | Mammalian PhCMV-driven constitutive 8xANR-NSP3 expression vector (PhCMV-(ANR)8-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
(ANR)8-NSP3
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pSL582
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL582载体质粒基本信息
pSL582质粒图谱载体图谱和pSL582载体序列质粒序列多克隆位点信息
pSL582质粒载体简介
pSL582质粒序列载体序列
LOCUS pSL582 7497 bp DNA circular SYN 14-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7497)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Jul 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7497
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 643..668
/label=185-WKG-602-CMV-F
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..900
/label=NheI
misc_feature 911
/label=New Feature
misc_feature 911
/label=New Feature
regulatory 917..926
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 925
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 926..988
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 989..1063
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1064..1126
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1127..1201
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1208..1270
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1271..1345
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1346..1408
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1409..1483
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1490..1552
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1553..1627
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1628..1690
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1691..1765
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1772..1834
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1835..1909
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1910..1972
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1973..2047
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 2054..2995
/label=NSP3
misc_feature 2054..2077
/label=NSP3
primer_bind complement(2065..2084)
/label=179-WKG-592-R
misc_feature 3018..3023
/label=EcoRI
misc_feature 3057..3062
/label=New Feature
misc_feature 3057..3062
/label=New Feature
polyA_signal 3094..3318
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3364..3792
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 3663..3682
/label=189-WKG-612-F
promoter 3806..4135
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4202..4996
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4246..4273)
/label=188-WKG-610-R
polyA_signal 5170..5291
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5336..5356)
/label=M13-rev
primer_bind complement(5340..5356)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5362..5384)
/label=LacO
protein_bind 5364..5380
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5388..5418)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5389..5418)
/label=lac
protein_bind 5433..5454
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5560..6500
/label=ColE1
/note="ColE1"
CDS complement(6501..7361)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7362..7466)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatggggga gctcgacgag ttggtttatc tcttagacgg
961 acccggatat gaccccattc attcagatgg ctccggaacg ggcagcggga ccggaagtgg
1021 caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact
1081 cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgatggaa gtggaactgg
1141 gtctgggact gggtccggca ccgggactac ttcaggcacc ggcactgggg gctccactgg
1201 ccataccggg gagctcgacg agttggttta tctcttagac ggacccggat atgaccccat
1261 tcattcagat ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg
1321 aaccggtact ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg
1381 ccccggttac gacccaatcc acagcgatgg aagtggaact gggtctggga ctgggtccgg
1441 caccgggact acttcaggca ccggcactgg gggctccact ggccataccg gggagctcga
1501 cgagttggtt tatctcttag acggacccgg atatgacccc attcattcag atggctccgg
1561 aacgggcagc gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag
1621 taccggcggt gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat
1681 ccacagcgat ggaagtggaa ctgggtctgg gactgggtcc ggcaccggga ctacttcagg
1741 caccggcact gggggctcca ctggccatac cggggagctc gacgagttgg tttatctctt
1801 agacggaccc ggatatgacc ccattcattc agatggctcc ggaacgggca gcgggaccgg
1861 aagtggcacc ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga
1921 cgaactcgtg tacctgttgg atggccccgg ttacgaccca atccacagcg atggaagtgg
1981 aactgggtct gggactgggt ccggcaccgg gactacttca ggcaccggca ctgggggctc
2041 cactggccat atgatgctca agatggagtc tacacagcag atggctagtt cgattattaa
2101 cacttctttt gaagccgcag tcgttgctgc cacttcaact cttgaactta tgggcattca
2161 atatgattat aatgaagttt atactagagt taagagcaaa tttgattacg taatggatga
2221 ttcaggtgtt aaaaacaatc tgttgggtaa agcggcaact attgatcagg cactgaatgg
2281 aaaatttggt tcagcagcaa gaaatagaaa ttggatggct gatactagaa ctacagctag
2341 actagatgaa gatgtgaaca aacttagaat gatgctatca tctaaaggaa ttgatcaaaa
2401 aatgagagta cttaatgcat gtttcaatgt gaagcgagtt ccaggtaaat catcatcaat
2461 aattaaatgt actagattaa tgagagataa gattgaacgt ggtgaagtcg aagtggatga
2521 ttcgtttgtt gaagaaaaaa tggaagttga cacaattgat tggaaatcta gatatgaaca
2581 attggaaaaa cgatttgaat cgctgaaaca aagggtgaat gaaaaatata caagttgggt
2641 gcaaaaagca aaaaaggtaa atgaaaatat gtactctctc caaaacgtta tatcacaaca
2701 gcagagccag attgctgatt tacaaaatta ctgtaataaa ttggaagttg atctacaaaa
2761 taaaattagt tcacttgtgt cttcagtcga atggtacctg aaatcaatgg aattgcctga
2821 tgaaattaag acagatattg aacaacaact aaattccatt gatgtaatca atccaattaa
2881 tgctatagat gattttgaat cactgatcag aaatattata ctcgactatg acagaatatt
2941 tttaatgttt aaaggattaa tgaggcaatg caactatgaa tatacgtatg aataggatcc
3001 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
3061 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
3121 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
3181 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
3241 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
3301 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
3361 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
3481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
3601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
3721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
3781 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
3841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
4141 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
4381 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
5161 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
5221 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
5281 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
5341 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
5401 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
5461 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
5521 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
5581 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
5641 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5701 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5761 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5821 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5881 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5941 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6001 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
6061 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
6121 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
6181 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6241 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
6421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
6481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
6541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
6601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6781 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
6841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
6901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
6961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
7021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
7081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
7141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
7201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
7261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
7321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
7381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
7441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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pSL582质粒载体应用举例
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