| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-IRES-Hyg, pLVX IRES Hygromycin |
| 质粒类型: | 慢病毒载体；哺乳动物细胞表达载体；双顺反子载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8519 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
| 3' 测序引物及序列: | IRES-R: CCTCACATTGCCAAAAGACG |
| 载体标签: | 无标签 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin(潮霉素) |
| 克隆菌株: | Stbl3 E.coli |
| 备注: |
pLVX-IRES-Hyg载体含IRES(内部核糖体结合位点）元件； Hyg抗性基因和目的基因以双顺反子的形式表达； CMV启动子驱动目的基因的过表达。 |
| 产品目录号: | 632185 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-IRES-Hyg 8519 bp DNA circular 8-NOV-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 632185,632182
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 4488..5079
/label=WPRE
rep_origin complement(6387..7060)
/label=pUC\origin
CDS complement(7205..8201)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5282..5918
/label=3'\LTR
CDS 3440..4474
/label=Hyg(R)
misc_feature 2841..3439
/label=IRES
misc_feature 2803..2840
/label=MCS
BASE COUNT 2188 a 2063 c 2200 g 2068 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa
2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
3241 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
3361 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
3421 gccacaaccc gtaccaaaga tggatagatc cggaaagcct gaactcaccg cgacgtctgt
3481 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
3541 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
3601 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
3661 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
3721 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
3781 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
3841 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
3901 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
3961 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
4021 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
4081 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
4141 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
4201 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
4261 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
4321 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
4381 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
4441 acgccccagc actcgtccga gggcaaagga atagacgcgt ctggaacaat caacctctgg
4501 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
4561 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
4621 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
4681 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
4741 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
4801 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
4861 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
4921 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
4981 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
5041 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
5101 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
5161 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
5221 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
5281 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
5341 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
5401 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
5461 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
5521 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
5581 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
5641 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
5701 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
5941 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct
6001 agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
6061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
6121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
6181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
6241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
6301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
6361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
6421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
6481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
6541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
6601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
6661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
6721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
6781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
6901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
7021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
7081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
7141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
7201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
7261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
7321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
7381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
7441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
7501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
7561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
7621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
7681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
7741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
7801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
7861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
7921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
7981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
8041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
8101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
8161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
8221 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
8281 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
8341 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
8401 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
8461 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
