pLVX-IRES-Hyg

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-IRES-Hyg, pLVX IRES Hygromycin
质粒类型: 慢病毒载体;哺乳动物细胞表达载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8519 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
载体标签: 无标签
载体抗性: 氨苄青霉素
筛选标记: Hygromycin(潮霉素)
克隆菌株: Stbl3 E.coli
备注: pLVX-IRES-Hyg载体含IRES(内部核糖体结合位点)元件;
Hyg抗性基因和目的基因以双顺反子的形式表达;
CMV启动子驱动目的基因的过表达。
产品目录号: 632185
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-IRES-Hyg载体图谱



pLVX-IRES-Hyg多克隆位点

pLVX-IRES-Hyg载体特征

载体简介

载体序列

LOCUS       pLVX-IRES-Hyg   8519 bp    DNA   circular             8-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632185,632182
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4488..5079
                     /label=WPRE
     rep_origin      complement(6387..7060)
                     /label=pUC\origin
     CDS             complement(7205..8201)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5282..5918
                     /label=3'\LTR
     CDS             3440..4474
                     /label=Hyg(R)
     misc_feature    2841..3439
                     /label=IRES
     misc_feature    2803..2840
                     /label=MCS
BASE COUNT     2188 a      2063 c      2200 g      2068 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 
     2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 
     2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 
     2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 
     3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 
     3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 
     3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 
     3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 
     3241 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 
     3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 
     3361 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 
     3421 gccacaaccc gtaccaaaga tggatagatc cggaaagcct gaactcaccg cgacgtctgt 
     3481 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg 
     3541 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa 
     3601 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc 
     3661 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat 
     3721 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt 
     3781 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag 
     3841 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat 
     3901 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag 
     3961 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt 
     4021 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat 
     4081 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa 
     4141 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg 
     4201 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct 
     4261 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg 
     4321 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg 
     4381 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg 
     4441 acgccccagc actcgtccga gggcaaagga atagacgcgt ctggaacaat caacctctgg 
     4501 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 
     4561 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 
     4621 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 
     4681 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 
     4741 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 
     4801 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 
     4861 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 
     4921 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 
     4981 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 
     5041 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga 
     5101 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc 
     5161 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt 
     5221 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg 
     5281 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca 
     5341 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc 
     5401 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc 
     5461 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc 
     5521 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg 
     5581 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc 
     5641 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag 
     5701 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct 
     5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 
     5941 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct 
     6001 agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 
     6061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     6121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     6181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     6241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     6301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     6361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     6421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     6481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     6541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     6601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     6661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     6721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     6781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     6841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     6901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     6961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     7021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     7081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     7141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     7201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     7261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     7321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     7381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     7441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     7501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     7561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     7621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     7681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     7741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     7801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     7861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     7921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     7981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     8041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     8101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     8161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 
     8221 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     8281 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     8341 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 
     8401 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 
     8461 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt 
//

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