pESC-Leu

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent 安捷伦
载体名称: pESC-Leu, pESC Leu
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1和GAL10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7758 bp
5' 测序引物及序列: GAL1-F: ATTTTCGGTTTGTATTACTTC;
GAL10-F: GGTGGTAATGCCATGTAATATG
3' 测序引物及序列: GALI-R: GTTCTTAATACTAACATAACT
GAL10-R: GGCAAGGTAGACAAGCCGACAAC
载体标签: C-Flag, C-Myc
载体抗性: 氨苄
筛选标记: Leu2
备注: 利用半乳糖诱导,可以同时使两个基因在酿酒酵母中表达。
产品目录号: 217452
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pESC-Leu载体图谱

pESC-Leu多克隆位点

载体简介

载体序列

LOCUS       pESC-LEU                7758 bp    DNA     circular SYN 10-OCT-2005
DEFINITION  Cloning vector pESC-LEU, complete sequence.
ACCESSION   AF063849
VERSION     AF063849.1  GI:3228559
SOURCE      Cloning vector pESC-LEU.
  ORGANISM  Cloning vector pESC-LEU
            artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Cloning vector pESC-LEU"
                     /mol_type="genomic DNA"
                     /specific_host="Escherichia coli K12"
                     /db_xref="taxon:76666"
                     /vntifkey="98"
     CDS             complement(5359..6216)
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      6350..7505
                     /vntifkey="33"
                     /label=2\micron\ori
     CDS             complement(663..1766)
                     /vntifkey="4"
                     /label=LEU2
                     /note="yeast LEU2 ORF"
     terminator      complement(3012..3176)
                     /vntifkey="43"
                     /label=ADH1\terminator
     terminator      4165..4354
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(2606..2912)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3607..4061
                     /vntifkey="29"
                     /label=GAL1\promoter
     promoter        complement(3395..3606)
                     /vntifkey="29"
                     /label=GAL10\promoter
     misc_feature    4103..4138
                     /vntifkey="21"
                     /label=c-myc\tag
     misc_feature    complement(3325..3351)
                     /vntifkey="21"
                     /label=FLAG\tag
     misc_feature    complement(3307..3369)
                     /vntifkey="21"
                     /label=MCS1
     misc_feature    4063..4160
                     /vntifkey="21"
                     /label=MCS2
     rep_origin      4541..5208
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     2201 a      1663 c      1649 g      2245 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctgaatt ggagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga 
     3061 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac 
     3121 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta 
     3181 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat 
     3241 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa 
     3301 gaattgttaa ttaagagctc agatcttatc gtcgtcatcc ttgtaatcca tcgatactag 
     3361 tgcggccgcc ctttagtgag ggttgaattc gaattttcaa aaattcttac tttttttttg 
     3421 gatggacgca aagaagttta ataatcatat tacatggcat taccaccata tacatatcca 
     3481 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc 
     3541 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta 
     3601 tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc 
     3661 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca 
     3721 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt 
     3781 ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg 
     3841 ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt 
     3901 ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc 
     3961 aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg 
     4021 ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatccgt aatacgactc 
     4081 actatagggc ccgggcgtcg acatggaaca gaagttgatt tccgaagaag acctcgagta 
     4141 agcttggtac cgcggctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc 
     4201 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat 
     4261 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa 
     4321 aaccttgctt gagaaggttt tgggacgctc gaagatccag ctgcattaat gaatcggcca 
     4381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     4441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     4501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     4561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     4621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     4681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     4741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     4801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     4861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     4921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     4981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     5041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     5101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     5161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     5221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     5281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     5341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     5401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     5461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     5521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     5581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     5641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     5701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     5761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     5821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     5881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     5941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     6001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     6061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     6121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     6181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     6241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     6301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg 
     6361 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc 
     6421 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa 
     6481 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa 
     6541 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca 
     6601 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa 
     6661 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata 
     6721 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc 
     6781 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg 
     6841 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat 
     6901 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg 
     6961 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt 
     7021 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga 
     7081 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga 
     7141 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa 
     7201 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg 
     7261 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta 
     7321 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg 
     7381 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat 
     7441 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt 
     7501 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg 
     7561 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt 
     7621 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg 
     7681 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     7741 tcacgaggcc ctttcgtc 
//

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