pLVX-DsRed-Monomer-N1

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DsRed-Monomer-N1
质粒类型: 慢病毒表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8751 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: --
载体标签: DsRed-Monomer(C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: pLVX-DsRed-Monomer-N1载体表达C端DsRed-Monomer融合蛋白;
DsRed-Monomer是DsRed的单体突变体,与DsRed相比,编码序列经过了人工优化,适用于在哺乳动物细胞中的高水平表达;
CMV启动子是过表达启动子。
产品目录号: 632152
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DsRed-Monomer-N1载体图谱



pLVX-DsRed-Monomer-N1多克隆位点

pLVX-DsRed-Monomer-N1载体特征

载体简介

Description
pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site (MCS),located upstream of the DsRed-Monomer coding sequence, are expressed as N-terminal fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE) located just upstream of the MCS. Lentiviral particles derived from the vector allow the expression of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to
optimize infection protocols.

pLVX-DsRed-Monomer-N1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and nhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-DsRed-Monomer-N1 also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more efficient transduction (3).

In addition to lentiviral elements, pLVX-DsRed-Monomer-N1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

Use
pLVX-DsRed-Monomer-N1 constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HTX Packaging System (Cat. Nos. 631247 and 631249). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

载体序列

LOCUS       pLVX-DsRed-Monomer-N1   8751 bp    DNA   circular             9-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632152
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4720..5311
                     /label=WPRE
     rep_origin      complement(6619..7292)
                     /label=pUC\origin
     CDS             complement(7437..8433)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5514..6150
                     /label=3'\LTR
     misc_feature    2814..2868
                     /label=MCS
     CDS             2881..3558
                     /label=DsRed-Monomer
     promoter        3578..4086
                     /label=PGK\Promoter
     CDS             4107..4706
                     /label=Puro(R)
BASE COUNT     2168 a      2279 c      2317 g      1987 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc 
     2881 atggacaaca ccgaggacgt catcaaggag ttcatgcagt tcaaggtgcg catggagggc 
     2941 tccgtgaacg gccactactt cgagatcgag ggcgagggcg agggcaagcc ctacgagggc 
     3001 acccagaccg ccaagctgca ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 
     3061 ctgtcccccc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc 
     3121 gactacatga agctgtcctt ccccgagggc ttcacctggg agcgctccat gaacttcgag 
     3181 gacggcggcg tggtggaggt gcagcaggac tcctccctgc aggacggcac cttcatctac 
     3241 aaggtgaagt tcaagggcgt gaacttcccc gccgacggcc ccgtaatgca gaagaagact 
     3301 gccggctggg agccctccac cgagaagctg tacccccagg acggcgtgct gaagggcgag 
     3361 atctcccacg ccctgaagct gaaggacggc ggccactaca cctgcgactt caagaccgtg 
     3421 tacaaggcca agaagcccgt gcagctgccc ggcaaccact acgtggactc caagctggac 
     3481 atcaccaacc acaacgagga ctacaccgtg gtggagcagt acgagcacgc cgaggcccgc 
     3541 cactccggct cccagtagag cggccgcgac tctagataat tctaccgggt aggggaggcg 
     3601 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 
     3661 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 
     3721 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 
     3781 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 
     3841 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 
     3901 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 
     3961 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 
     4021 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 
     4081 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 
     4141 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 
     4201 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 
     4261 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 
     4321 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 
     4381 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 
     4441 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 
     4501 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 
     4561 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 
     4621 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 
     4681 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa 
     4741 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     4801 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     4861 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     4921 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     4981 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     5041 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     5101 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 
     5161 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     5221 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     5281 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa 
     5341 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag 
     5401 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa 
     5461 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 
     5521 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 
     5581 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 
     5641 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 
     5701 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 
     5761 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 
     5821 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 
     5881 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 
     5941 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 
     6001 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     6061 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     6121 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     6181 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta 
     6241 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
     6301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
     6361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     6421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     6481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     6541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     6601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     6661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     6721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     6781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     6841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 
     6901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     6961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     7021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     7081 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 
     7141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     7201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     7261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     7321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     7381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     7441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     7501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     7561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     7621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     7681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     7741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     7801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     7861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     7921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     7981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     8041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     8101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     8161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     8221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     8281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     8341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     8401 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat 
     8461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     8521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     8581 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat 
     8641 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt 
     8701 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t 
//

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