| 质粒名称: | OKSIM |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 24603 |
| 存储实验室: | Jose Cibelli |
|
载体骨架基本信息 |
|
| 载体名称: | pSin4-EF2-IRES-Pur |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6777 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，Cre/Lox |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Oct4 KLF4 Sox2 c-Myc |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5600 bp |
| 基因突变: | Oct4-2A-KLF4-2A-Sox2-IRES-Myc |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Mlu I(未破坏) |
| 3' 克隆位点: | Pme I (未破坏) |
| 5' 测序引物 | EF-1a Forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | OKSIM was a gift from Jose Cibelli (Addgene plasmid # 24603 ; http://n2t.net/addgene:24603 ; RRID:Addgene_24603) |
| 参考文献部分: | Human Induced Pluripotent Stem Cells Produced Under Xeno-Free Conditions. Ross PJ, Suhr S, Rodriguez RM, Chang EA, Wang K, Siripattarapravat K, Ko T, Cibelli JB. Stem Cells Dev. 2009 Dec 23. ():. 10.1089/scd.2009.0459 PubMed 20030562 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS OKSIM 12417 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS OKSIM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12417)
AUTHORS Ross PJ, Suhr S, Rodriguez RM, Chang EA, Wang K, Siripattarapravat
K, Ko T, Cibelli JB
TITLE Human Induced Pluripotent Stem Cells Produced Under Xeno-Free
Conditions.
JOURNAL Stem Cells Dev. 2009 Dec 23. ():.
PUBMED 20030562
REFERENCE 2 (bases 1 to 12417)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12417
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(157..174)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 384..403
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 608..1927
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
primer_bind complement(1978..1994)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1979..1995)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 2008..2596
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2061..2081)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2479..2490)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 2740..2773
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 2793..2973
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(2996..3013)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3002..3226
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
regulatory 3269..3278
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter 3322..3426
/gene="bla"
/label=AmpR promoter
CDS 3427..4287
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3645..3664)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4458..5046
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4947..4966
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 5205..5838
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 5885..6010
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6503..6736
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 7252..7369
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7498..8676
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 7729..8667
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 8624..8644
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 8690..8710
/label=T3
/note="T3 promoter, forward primer"
promoter 8692..8710
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 9840..9905
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 11352..11417
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
misc_feature 12410..563
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
ORIGIN
1 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
61 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
121 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
181 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
241 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
301 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
361 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
421 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
481 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
541 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccatggc
601 gcgtggtatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc
661 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag
721 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac
781 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac
841 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca
901 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga
961 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt
1021 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga
1081 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct
1141 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc
1201 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc
1261 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct
1321 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga
1381 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga
1441 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa
1501 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga
1561 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa
1621 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac
1681 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg
1741 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa
1801 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga
1861 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg
1921 tgcgtaagtt taaacggatc catcgatact agttcgaatt cgatatcaag cttatcgcga
1981 taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
2041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
2101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
2161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
2221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
2281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
2341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
2401 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
2461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
2521 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
2581 tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
2641 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
2701 ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta tagcatacat
2761 tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
2821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
2881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
2941 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gagctcgctg atcagcctcg
3001 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
3061 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
3121 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
3181 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
3241 agaaccagct gggggcgcgc ccctcgaggc cgccatggtc atagctgttt gacgtcaggt
3301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
3361 aatatgtatc cgctcacgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
3421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
3481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
3541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
3601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
3661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
3721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
3781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
3841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
3901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
3961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
4021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
4081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
4141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
4201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
4261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
4321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
4381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
4441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
4621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
4741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
4921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
4981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5041 tggaaaaacg ccagcaacgc ggccttttta cggttctggc cttttgcacg gaccgccact
5101 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
5161 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
5221 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
5281 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
5341 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
5401 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
5461 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
5521 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
5581 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
5641 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
5701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
5761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
5821 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
5881 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
5941 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
6001 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
6061 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
6121 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
6181 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
6241 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
6301 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
6361 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
6421 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
6481 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
6541 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
6601 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
6661 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
6721 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
6781 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
6841 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
6901 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
6961 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
7021 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
7081 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
7141 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
7201 tagtgaacgg atctcgacgg tatcgccaca aatggcagta ttcatccaca attttaaaag
7261 aaaggggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
7321 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
7381 cagggacagc agagatccac tttggatcga taagctttgc aaagatggat aaagttttaa
7441 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
7501 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
7561 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
7621 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
7681 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
7741 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
7801 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
7861 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
7921 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
7981 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
8041 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
8101 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
8161 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
8221 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
8281 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
8341 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
8401 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
8461 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
8521 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
8581 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
8641 gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa ttcgcctagg caattaaccc
8701 tcactaaagg gaaggccgcc tgggccacgc gtgccatggc gggacacctg gcttcggatt
8761 tcgccttctc gccccctcca ggtggtggag gtgatgggcc aggggggccg gagccgggct
8821 gggttgatcc tcggacctgg ctaagcttcc aaggccctcc tggagggcca ggaatcgggc
8881 cgggggttgg gccaggctct gaggtgtggg ggattccccc atgccccccg ccgtatgagt
8941 tctgtggggg gatggcgtac tgtgggcccc aggttggagt ggggctagtg ccccaaggcg
9001 gcttggagac ctctcagcct gagggtgaag caggagtcgg ggtggagagc aactccgatg
9061 gggcctcccc ggagccctgc accgtcaccc ctggtgccgt gaagctggag aaggagaagc
9121 tggagcaaaa cccggaggag tcccaggaca tcaaagctct gcagaaagaa ctcgagcaat
9181 ttgccaagct cctgaagcag aagaggatca ccctgggata tacacaggcc gatgtggggc
9241 tcaccctggg ggttctattt gggaaggtat tcagccaaac gaccatctgc cgctttgagg
9301 ctctgcagct tagcttcaag aacatgtgta agctgcggcc cttgctgcag aagtgggtgg
9361 aggaagctga caacaatgaa aatcttcagg agatatgcaa agcagaaacc ctcgtgcagg
9421 cccgaaagag aaagcgaacc agtatcgaga accgagtgag aggcaacctg gagaatttgt
9481 tcctgcagtg cccgaaaccc acactgcagc agatcagcca catcgcccag cagcttgggc
9541 tcgagaagga tgtggtccga gtgtggttct gtaaccggcg ccagaagggc aagcgatcaa
9601 gcagcgacta tgcacaacga gaggattttg aggctgctgg gtctcctttc tcagggggac
9661 cagtgtcctt tcctctggcc ccagggcccc attttggtac cccaggctat gggagccctc
9721 acttcactgc actgtactcc tcggtccctt tccctgaggg ggaagccttt ccccctgtct
9781 ctgtcaccac tctgggctct cccatgcatt caaacggggc gcgtaaaatt gtcgctcctg
9841 tcaaacaaac tcttaacttt gatttactca aactggctgg ggatgtagaa agcaatccag
9901 gtccaggggc gcgtggtatg gctgtcagcg acgcgctgct cccatctttc tccacgttcg
9961 cgtctggccc ggcgggaagg gagaagacac tgcgtcaagc aggtgccccg aataaccgct
10021 ggcgggagga gctctcccac atgaagcgac ttcccccagt gcttcccggc cgcccctatg
10081 acctggcggc ggcgaccgtg gccacagacc tggagagcgg cggagccggt gcggcttgcg
10141 gcggtagcaa cctggcgccc ctacctcgga gagagaccga ggagttcaac gatctcctgg
10201 acctggactt tattctctcc aattcgctga cccatcctcc ggagtcagtg gccgccaccg
10261 tgtcctcgtc agcgtcagcc tcctcttcgt cgtcgccgtc gagcagcggc cctgccagcg
10321 cgccctccac ctgcagcttc acctatccga tccgggccgg gaacgacccg ggcgtggcgc
10381 cgggcggcac gggcggaggc ctcctctatg gcagggagtc cgctccccct ccgacggctc
10441 ccttcaacct ggcggacatc aacgacgtga gcccctcggg cggcttcgtg gccgagctcc
10501 tgcggccaga attggacccg gtgtacattc cgccgcagca gccgcagccg ccaggtggcg
10561 ggctgatggg caagttcgtg ctgaaggcgt cgctgagcgc ccctggcagc gagtacggca
10621 gcccgtcggt catcagcgtc agcaaaggca gccctgacgg cagccacccg gtggtggtgg
10681 cgccctacaa cggcgggccg ccgcgcacgt gccccaagat caagcaggag gcggtctctt
10741 cgtgcaccca cttgggcgct ggaccccctc tcagcaatgg ccaccggccg gctgcacacg
10801 acttccccct ggggcggcag ctccccagca ggactacccc gaccctgggt cttgaggaag
10861 tgctgagcag cagggactgt caccctgccc tgccgcttcc tcccggcttc catccccacc
10921 cggggcccaa ttacccatcc ttcctgcccg atcagatgca gccgcaagtc ccgccgctcc
10981 attaccaaga gctcatgcca cccggttcct gcatgccaga ggagcccaag ccaaagaggg
11041 gaagacgatc gtggccccgg aaaaggaccg ccacccacac ttgtgattac gcgggctgcg
11101 gcaaaaccta cacaaagagt tcccatctca aggcacacct gcgaacccac acaggtgaga
11161 aaccttacca ctgtgactgg gacggctgtg gatggaaatt cgcccgctca gatgaactga
11221 ccaggcacta ccgtaaacac acggggcacc gcccgttcca gtgccaaaaa tgcgaccgag
11281 cattttccag gtcggaccac ctcgccttac acatgaagag gcattttggg gcgcgtaaaa
11341 ttgtcgctcc tgtcaaacaa actcttaact ttgatttact caaactggct ggggatgtag
11401 aaagcaatcc aggtccaggg gcgcgtggta tgtacaacat gatggagacg gagctgaagc
11461 cgccgggccc gcagcaaact tcggggggcg gcggcggcaa ctccaccgcg gcggcggccg
11521 gcggcaacca gaaaaacagc ccggaccgcg tcaagcggcc catgaatgcc ttcatggtgt
11581 ggtcccgcgg gcagcggcgc aagatggccc aggagaaccc caagatgcac aactcggaga
11641 tcagcaagcg cctgggcgcc gagtggaaac ttttgtcgga gacggagaag cggccgttca
11701 tcgacgaggc taagcggctg cgagcgctgc acatgaagga gcacccggat tataaatacc
11761 ggccccggcg gaaaaccaag acgctcatga agaaggataa gtacacgctg cccggcgggc
11821 tgctggcccc cggcggcaat agcatggcga gcggggtcgg ggtgggcgcc ggcctgggcg
11881 cgggcgtgaa ccagcgcatg gacagttacg cgcacatgaa cggctggagc aacggcagct
11941 acagcatgat gcaggaccag ctgggctacc cgcagcaccc gggcctcaat gcgcacggcg
12001 cagcgcagat gcagcccatg caccgctacg acgtgagcgc cctgcagtac aactccatga
12061 ccagctcgca gacctacatg aacggctcgc ccacctacag catgtcctac tcgcagcagg
12121 gcacccctgg catggctctt ggctccatgg gttcggtggt caagtccgag gccagctcca
12181 gcccccctgt ggttacctct tcctcccact ccagggcgcc ctgccaggcc ggggacctcc
12241 gggacatgat cagcatgtat ctccccggcg ccgaggtgcc ggaacccgcc gcccccagca
12301 gacttcacat gtcccagcac taccagagcg gcccggtgcc cggcacggcc attaacggca
12361 cactgcccct ctcacacatg tgagtttaaa tctagggcgg ccaattccgc ccctctc
//
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