| 质粒名称: | pAAVLINK-EF1a-5'-COL1A2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001671 |
| 产品用途: | 5' AAVLINK plasmid for COL1A2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-COL1A2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-COL1A2载体质粒基本信息
pAAVLINK-EF1a-5'-COL1A2质粒图谱载体图谱和pAAVLINK-EF1a-5'-COL1A2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-COL1A2质粒载体简介
pAAVLINK-EF1a-5'-COL1A2质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-COL1A2 5355 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5355)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5355
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..2495
/codon_start=1
/label=COL1A2
/translation="MLSFVDTRTLLLLAVTLCLATCQSLQEETVRKGPAGDRGPRGERG
PPGPPGRDGEDGPTGPPGPPGPPGPPGLGGNFAAQYDGKGVGLGPGPMGLMGPRGPPGA
AGAPGPQGFQGPAGEPGEPGQTGPAGARGPAGPPGKAGEDGHPGKPGRPGERGVVGPQG
ARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGENGTPGQTGARGLPGER
GRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGEIGAVGNAGPAGPAGP
RGEVGLPGLSGPVGPPGNPGANGLTGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATG
ARGLVGEPGPAGSKGESGNKGEPGSAGPQGPPGPSGEEGKRGPNGEAGSAGPPGPPGLR
GSPGSRGLPGADGRAGVMGPPGSRGASGPAGVRGPNGDAGRPGEPGLMGPRGLPGSPGN
IGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPTGDPGKNGDKGHAGLAG
ARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLH
GEFGLPGPAGPRGERGPP"
misc_feature 893..898
/label=SDS
misc_feature 911..916
/label=SDS
misc_feature 1121..1126
/label=SDS
misc_feature 1598..1603
/label=SDS
misc_feature 1823..1828
/label=SDS
misc_feature 1895..1900
/label=SDS
misc_feature 1967..1972
/label=SDS
misc_feature 2309..2314
/label=SDS
intron 2496..2536
/label=Splicing Donor
misc_feature 2540..2573
/label=lox2272-JT1571
repeat_region 2618..2758
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2618..2747
/label=AAV2 ITR
rep_origin 2833..3288
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3570..3674
/gene="bla"
/label=AmpR promoter
CDS 3675..4535
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4706..5294
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgctcag
721 ctttgtggat acgcggactt tgttgctgct tgcagtaacc ttatgcctag caacatgcca
781 atctttacaa gaggaaactg taagaaaggg cccagccgga gatagaggac cacgtggaga
841 aaggggtcca ccaggccccc caggcagaga tggtgaagat ggtcccacag gccctcctgg
901 tccacctggt cctcctggcc cccctggtct cggtgggaac tttgctgctc agtatgatgg
961 aaaaggagtt ggacttggcc ctggaccaat gggcttaatg ggacctagag gcccacctgg
1021 tgcagctgga gccccaggcc ctcaaggttt ccaaggacct gctggtgagc ctggtgaacc
1081 tggtcaaact ggtcctgcag gtgctcgtgg tccagctggc cctcctggca aggctggtga
1141 agatggtcac cctggaaaac ccggacgacc tggtgagaga ggagttgttg gaccacaggg
1201 tgctcgtggt ttccctggaa ctcctggact tcctggcttc aaaggcatta ggggacacaa
1261 tggtctggat ggattgaagg gacagcccgg tgctcctggt gtgaagggtg aacctggtgc
1321 ccctggtgaa aatggaactc caggtcaaac aggagcccgt gggcttcctg gtgagagagg
1381 acgtgttggt gcccctggcc cagctggtgc ccgtggcagt gatggaagtg tgggtcccgt
1441 gggtcctgct ggtcccattg ggtctgctgg ccctccaggc ttcccaggtg cccctggccc
1501 caagggtgaa attggagctg ttggtaacgc tggtcctgct ggtcccgccg gtccccgtgg
1561 tgaagtgggt cttccaggcc tctccggccc cgttggacct cctggtaatc ctggagcaaa
1621 cggccttact ggtgccaagg gtgctgctgg ccttcccggc gttgctgggg ctcccggcct
1681 ccctggaccc cgcggtattc ctggccctgt tggtgctgcc ggtgctactg gtgccagagg
1741 acttgttggt gagcctggtc cagctggctc caaaggagag agcggtaaca agggtgagcc
1801 cggctctgct gggccccaag gtcctcctgg tcccagtggt gaagaaggaa agagaggccc
1861 taatggggaa gctggatctg ccggccctcc aggacctcct gggctgagag gtagtcctgg
1921 ttctcgtggt cttcctggag ctgatggcag agctggcgtc atgggccctc ctggtagtcg
1981 tggtgcaagt ggccctgctg gagtccgagg acctaatgga gatgctggtc gccctgggga
2041 gcctggtctc atgggaccca gaggtcttcc tggttcccct ggaaatatcg gccccgctgg
2101 aaaagaaggt cctgtcggcc tccctggcat cgacggcagg cctggcccaa ttggccccgc
2161 tggagcaaga ggagagcctg gcaacattgg attccctgga cccaaaggcc ccactggtga
2221 tcctggcaaa aacggtgata aaggtcatgc tggtcttgct ggtgctcggg gtgctccagg
2281 tcctgatgga aacaatggtg ctcagggacc tcctggacca cagggtgttc aaggtggaaa
2341 aggtgaacag ggtccccctg gtcctccagg cttccagggt ctgcctggcc cctcaggtcc
2401 cgctggtgaa gttggcaaac caggagaaag gggtctccat ggtgagtttg gtctccctgg
2461 tcctgctggt ccaagagggg aacgcggtcc cccaggtaag tatcaaggtt acaagacagg
2521 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
2581 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
2641 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
2701 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
2761 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
2821 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
2881 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
2941 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
3001 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
3061 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
3121 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
3181 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
3241 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
3301 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
3361 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
3421 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
3481 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
3541 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
3601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
3661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
3721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
3781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
3841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
3901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
3961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
4021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
4081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
4141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
4201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
4261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
4321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
4381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
4441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
4501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
4561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
4621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
4681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
4741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
4801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
4861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
4921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
4981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
5221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
5281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
5341 cttttgctca catgt
//
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