pBTB-1

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品人: Lynch,M.D. and Gill,R.T.
载体名称: pBTB-1
质粒类型: 广宿主载体;克隆载体;原核表达载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: araBAD
载体大小: 3784 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
克隆菌株: DH5α 等
宿主细胞(系): 多个菌属
备注: --
产品目录号: --
组成型/诱导型: 诱导型

载体质粒图谱和多克隆位点信息

pBTB-1载体图谱



载体简介

载体序列

LOCUS       DQ058729                3784 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBTB-1, complete sequence.
ACCESSION   DQ058729
VERSION     DQ058729.1  GI:69048308
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Broad host range vector pBTB-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:334961"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            872..1534
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             872..1534
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99717.1"
                     /db_xref="GI:69048309"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            1631..2491
                     /gene="bla"
                     /note="beta-lactam resistance"
     CDS             1631..2491
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAY99718.1"
                     /db_xref="GI:69048310"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      2507..2538
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(2566..3495)
                     /gene="araC"
     CDS             complement(2566..3495)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99719.1"
                     /db_xref="GI:69048311"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      3503..3770
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1801 tttacgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1921 gaatgacttg gttgagtact caccagtcac agaaaagcat ctcacggatg gcatgacagt
     1981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2041 ggcaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggatc
     2281 acttctgcgc tcggccctcc cggctggctg gtttattgct gataaatctg gagccggtga
     2341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgcatcgt
     2401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2461 gataggtgcc tcactgatta agcattggta atgaggatcc ccctcaagtc aaaagcctcc
     2521 ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat gatttttatg acaacttgac
     2581 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
     2641 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
     2701 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
     2761 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
     2821 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
     2881 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
     2941 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
     3001 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
     3061 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
     3121 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
     3181 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
     3241 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
     3301 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
     3361 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
     3421 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa
     3481 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
     3541 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
     3601 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
     3661 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
     3721 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagaatt
     3781 cgtc
//

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