PABE-2

LOCUS       PABE-2               10294 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    PABE-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2041..6141
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6054..6062
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6154..6174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6232..6408
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(6962..6978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6962..6978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6975..6997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6986..7002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7010..7040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7055..7076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7193..7210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7364..7952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7444..7463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8123..8983)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8746..8765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8984..9088)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg
       61 cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt
      121 tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt
      181 tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattaa
      241 ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca
      301 tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat
      361 gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg
      421 atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa
      481 ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta
      541 cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt
      601 actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac
      661 ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg
      721 atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt
      781 tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaaag
      841 cttatgtccg aggtggagtt ctctcacgag tattggatga ggcacgctct tacacttgct
      901 aagagagctt gggacgaaag agaagtgcca gttggcgccg ttcttgtgca taataatagg
      961 gtgatcggcg agggttggaa tagaccaatt ggaaggcatg atccaacagc tcacgcagag
     1021 attatggctc tcagacaagg cggcctcgtt atgcagaact acaggctcat tgacgctaca
     1081 ctctacgtga cactcgaacc ttgcgttatg tgcgccggag ctatgattca ttctaggatt
     1141 ggcagggtcg tgtttggagc tagggacgct aaaacaggag ccgccggatc tcttatggac
     1201 gtgttgcatc atccaggcat gaaccatagg gtggagatta cagagggcat tcttgcagac
     1261 gagtgcgctg ctcttctttc cgatttcttc aggatgagaa ggcaggagat taaggcccag
     1321 aagaaggctc agtcttctac agatagcgga ggatcttccg gaggatctag cggctccgag
     1381 acaccaggaa catccgaaag cgctacacca gaatctagcg gaggctcttc cggaggatct
     1441 tctgaagtgg agttctccca cgagtattgg atgaggcacg ctcttacact tgctaaaagg
     1501 gctagggacg aaagggaagt tccagttgga gctgttctcg tgctcaataa cagggtgatt
     1561 ggcgagggtt ggaatagagc cattggactc catgatccaa cagctcacgc agagattatg
     1621 gctcttagac aaggcggcct cgttatgcag aattacagac tcatcgacgc cacactctac
     1681 gttaccttcg aaccttgcgt tatgtgcgcc ggagctatga tccattctag gattggcagg
     1741 gtcgtgttcg gcgttagaaa cgctaagaca ggagctgcag gctctcttat ggacgttctt
     1801 cattacccag gcatgaatca tagagtggag atcacagaag gcattcttgc agacgagtgc
     1861 gcagctctcc tttgctattt cttcaggatg ccgaggcaag ttttcaacgc tcagaagaag
     1921 gcccagtctt ctacagattc cggcggatct tctggaggat ctagcggctc cgagacacca
     1981 ggaacatccg aatccgctac accagagtct tctggaggat ctagcggagg atctcttaag
     2041 gacaagaagt actcgatcgg cctcgccatt gggactaact ctgttggctg ggccgtgatc
     2101 accgacgagt acaaggtgcc ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat
     2161 tccatcaaga agaatctcat tggcgctctc ctgttcgaca gcggcgagac ggctgaggct
     2221 acgcggctca agcgcaccgc ccgcaggcgg tacacgcgca ggaagaatcg catctgctac
     2281 ctgcaggaga ttttctccaa cgagatggcg aaggttgacg attctttctt ccacaggctg
     2341 gaggagtcat tcctcgtgga ggaggataag aagcacgagc ggcatccaat cttcggcaac
     2401 attgtcgacg aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag
     2461 ctcgtggact ccacagataa ggcggacctc cgcctgatct acctcgctct ggcccacatg
     2521 attaagttca ggggccattt cctgatcgag ggggatctca acccggacaa tagcgatgtt
     2581 gacaagctgt tcatccagct cgtgcagacg tacaaccagc tcttcgagga gaaccccatt
     2641 aatgcgtcag gcgtcgacgc gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc
     2701 ctcgagaacc tgatcgccca gctgccgggc gagaagaaga acggcctgtt cgggaatctc
     2761 attgcgctca gcctggggct cacgcccaac ttcaagtcga atttcgatct cgctgaggac
     2821 gccaagctgc agctctccaa ggacacatac gacgatgacc tggataacct cctggcccag
     2881 atcggcgatc agtacgcgga cctgttcctc gctgccaaga atctgtcgga cgccatcctc
     2941 ctgtctgata ttctcagggt gaacaccgag attacgaagg ctccgctctc agcctccatg
     3001 atcaagcgct acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag
     3061 cagctccccg agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg
     3121 tacattgacg gcggggcctc tcaggaggag ttctacaagt tcatcaagcc gattctggag
     3181 aagatggacg gcacggagga gctgctggtg aagctcaatc gcgaggacct cctgaggaag
     3241 cagcggacat tcgataacgg cagcatccca caccagattc atctcgggga gctgcacgct
     3301 atcctgagga ggcaggagga cttctaccct ttcctcaagg ataaccgcga gaagatcgag
     3361 aagattctga ctttcaggat cccgtactac gtcggcccac tcgctagggg caactcccgc
     3421 ttcgcttgga tgacccgcaa gtcagaggag acgatcacgc cgtggaactt cgaggaggtg
     3481 gtcgacaagg gcgctagcgc tcagtcgttc atcgagagga tgacgaattt cgacaagaac
     3541 ctgccaaatg agaaggtgct ccctaagcac tcgctcctgt acgagtactt cacagtctac
     3601 aacgagctga ctaaggtgaa gtatgtgacc gagggcatga ggaagccggc tttcctgtct
     3661 ggggagcaga agaaggccat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt
     3721 aagcagctca aggaggacta cttcaagaag attgagtgct tcgattcggt cgagatctct
     3781 ggcgttgagg accgcttcaa cgcctccctg gggacctacc acgatctcct gaagatcatt
     3841 aaggataagg acttcctgga caacgaggag aatgaggata tcctcgagga cattgtgctg
     3901 acactcactc tgttcgagga ccgggagatg atcgaggagc gcctgaagac ttacgcccat
     3961 ctcttcgatg acaaggtcat gaagcagctc aagaggagga ggtacaccgg ctgggggagg
     4021 ctgagcagga agctcatcaa cggcattcgg gacaagcagt ccgggaagac gatcctcgac
     4081 ttcctgaaga gcgatggctt cgcgaaccgc aatttcatgc agctgattca cgatgacagc
     4141 ctcacattca aggaggatat ccagaaggct caggtgagcg gccaggggga ctcgctgcac
     4201 gagcatatcg cgaacctcgc tggctcgcca gctatcaaga aggggattct gcagaccgtg
     4261 aaggttgtgg acgagctggt gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt
     4321 gagatggccc gggagaatca gaccacgcag aagggccaga agaactcacg cgagaggatg
     4381 aagaggatcg aggagggcat taaggagctg gggtcccaga tcctcaagga gcacccggtg
     4441 gagaacacgc agctgcagaa tgagaagctc tacctgtact acctccagaa tggccgcgat
     4501 atgtatgtgg accaggagct ggatattaac aggctcagcg attacgacgt cgatcatatc
     4561 gttccacagt cattcctgaa ggatgactcc attgacaaca aggtcctcac caggtcggac
     4621 aagaaccggg gcaagtctga taatgttcct tcagaggagg tcgttaagaa gatgaagaac
     4681 tactggcgcc agctcctgaa tgccaagctg atcacgcagc ggaagttcga taacctcaca
     4741 aaggctgaga ggggcgggct ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg
     4801 gtcgagacac ggcagatcac taagcacgtt gcgcagattc tcgactcacg gatgaacact
     4861 aagtacgatg agaatgacaa gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag
     4921 ctcgtctccg acttcaggaa ggatttccag ttctacaagg ttcgggagat caacaattac
     4981 caccatgccc atgacgcgta cctgaacgcg gtggtcggca cagctctgat caagaagtac
     5041 ccaaagctcg agagcgagtt cgtgtacggg gactacaagg tttacgatgt gaggaagatg
     5101 atcgccaagt cggagcagga gattggcaag gctaccgcca agtacttctt ctactctaac
     5161 attatgaatt tcttcaagac agagatcact ctggccaatg gcgagatccg gaagcgcccc
     5221 ctcatcgaga cgaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg
     5281 accgtcagga aggttctctc catgccacaa gtgaatatcg tcaagaagac agaggtccag
     5341 actggcgggt tctctaagga gtcaattctg cctaagcgga acagcgacaa gctcatcgcc
     5401 cgcaagaagg actgggatcc gaagaagtac ggcgggttcg acagccccac tgtggcctac
     5461 tcggtcctgg ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag
     5521 gagctgctgg ggatcacgat tatggagcgc tccagcttcg agaagaaccc gatcgatttc
     5581 ctggaggcga agggctacaa ggaggtgaag aaggacctga tcattaagct ccccaagtac
     5641 tcactcttcg agctggagaa cggcaggaag cggatgctgg cttccgctgg cgagctgcag
     5701 aaggggaacg agctggctct gccgtccaag tatgtgaact tcctctacct ggcctcccac
     5761 tacgagaagc tcaagggcag ccccgaggac aacgagcaga agcagctgtt cgtcgagcag
     5821 cacaagcatt acctcgacga gatcattgag cagatttccg agttctccaa gcgcgtgatc
     5881 ctggccgacg cgaatctgga taaggtcctc tccgcgtaca acaagcaccg cgacaagcca
     5941 atcagggagc aggctgagaa tatcattcat ctcttcaccc tgacgaacct cggcgcccct
     6001 gctgctttca agtacttcga cacaactatc gatcgcaaga ggtacacaag cactaaggag
     6061 gtcctggacg cgaccctcat ccaccagtcg attaccggcc tctacgagac gcgcatcgac
     6121 ctgtctcagc tcgggggcga ctccggcggc agcccaaaga agaagcggaa ggtgtaggaa
     6181 ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     6241 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     6301 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     6361 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc ctaaagtccc
     6421 tatagatctt tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg agcccagacg
     6481 ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga tgctaaggca
     6541 gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt ggacggaagg
     6601 aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga agatggaaat
     6661 ttgatagagg tacgctacta tacttatact atacgctaag ggaatgcttg tatttatacc
     6721 ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc ccccgcttaa
     6781 aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat aaaacctcac
     6841 caaaatacga aagagttctt aactctaaag ataaaagatc tttcaagatc aaaactagtt
     6901 ccctcacacc ggtgacgggg atcgcatgcg atatctcgag atctagcttg gcgtaatcat
     6961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgccagtg
     9121 ccaagctaat tcgagctcgg tacctgaccc ggtcgtgccc ctctctagag ataatgagca
     9181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
     9241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
     9301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
     9361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
     9421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
     9481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
     9541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
     9601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
     9661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
     9721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
     9781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg atcgagagtt
     9841 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca
     9901 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg
     9961 gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc
    10021 ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga
    10081 tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc
    10141 ccccccctct ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc
    10201 tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc
    10261 gtacacggat gcgacctgta cgtcagacac gttc
//