| 质粒名称: | pACYC-T7-Nla-repeat-BbsI |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 180215 |
| 存储实验室: | Yan Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC |
| 载体出品公司: | Novagen |
| 空载体大小: | 2219bp |
| 完整质粒大小: | 2309bp |
| 载体类型: | Bacterial Expression, CRISPR |
| 载体抗性: | Chloramphenicol, 25 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 克隆方法: | Ligation Independent Cloning |
| 3’测序引物 |
GACTACCGGAAGCAGTGTGAC
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pACYC-T7-Nla-repeat-BbsI was a gift from Yan Zhang (Addgene plasmid # 180215 ; http://n2t.net/addgene:180215 ; RRID:Addgene_180215) |
| 参考文献部分: | Cas11 enables genome engineering in human cells with compact CRISPR-Cas3 systems. Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z, Zhang Y. Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi: 10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19. 10.1016/j.molcel.2021.12.032 PubMed 35051351 |
买家导航
pACYC-T7-Nla-repeat-BbsI载体质粒基本信息
pACYC-T7-Nla-repeat-BbsI质粒图谱载体图谱和pACYC-T7-Nla-repeat-BbsI载体序列质粒序列多克隆位点信息
pACYC-T7-Nla-repeat-BbsI质粒载体简介
pACYC-T7-Nla-repeat-BbsI质粒序列载体序列
LOCUS pACYC-T7-Nla-repeat-BbsI 2309 bp DNA circular SYN 14-APR-2025
DEFINITION For cloning Neisseria lactamica Type I-C crRNA for protein
purification in E.Coli.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2309)
AUTHORS Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z,
Zhang Y
TITLE Cas11 enables genome engineering in human cells with compact
CRISPR-Cas3 systems.
JOURNAL Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi:
10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19.
PUBMED 35051351
REFERENCE 2 (bases 1 to 2309)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.molcel.2021.12.032"; journalName: "Mol Cell"; date:
"2022-02-17- 17"; volume: "82"; issue: "4"; pages: "852-867.e5"
COMMENT Sequence Label: pACYC-T7-Nla-repeat-BbsI
FEATURES Location/Qualifiers
source 1..2309
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(364..1023)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 938..957
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(1024..1126)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(1518..1535)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1652..2197)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(1732..1751)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 attaatacga ctcactatag tcagccgcct ctaggcggct gtgtgttgaa acgggtcttc
61 attattccag aagacaatca gccgcctcta ggcggctgtg tgttgaaact tttttttcta
121 gatatcgctc aatactgacc atgctgaaac ctcaggcatt tgagaagcac acggtcacac
181 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
241 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt
301 attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa
361 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
421 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
481 gtcgccttgc gtataatatt tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt
541 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
601 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
661 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
721 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
781 ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat
841 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
901 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
961 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
1021 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
1081 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
1141 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
1201 gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa
1261 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
1321 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac
1381 atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga
1441 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
1501 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
1561 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
1621 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
1681 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
1741 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
1801 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
1861 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
1921 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
1981 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
2041 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
2101 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
2161 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
2221 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
2281 catacgatat aagttgtaat tctcatgtt
//
