| 质粒名称: | piRFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31857 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iRFP |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 948 bp |
| 基因突变: | T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI(克隆期间被破坏) |
| 3' 克隆位点: | KpnI(克隆期间被破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | piRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31857) |
| 参考文献部分: | Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS piRFP 4937 bp DNA SYN
DEFINITION piRFP
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4937
/organism="piRFP"
/mol_type="other DNA"
promoter 10..562
/label="CMV_immearly_promoter"
misc_feature 65..352
/label="CAG_enhancer"
CDS complement(447..953)
/label="ORF frame 1"
misc_feature 519..539
/label="CMV_fwd_primer"
promoter 520..589
/label="CMV_promoter"
CDS 624..1574
/label="ORF frame 3"
terminator 1618..1846
/label="SV40_PA_terminator"
misc_feature 1816..1835
/label="EBV_rev_primer"
rep_origin complement(1985..2291)
/label="f1_origin"
promoter 2370..2398
/label="AmpR_promoter"
misc_feature complement(2464..2484)
/label="pBABE_3_primer"
misc_feature complement(2470..2685)
/label="SV40_enhancer"
promoter 2482..2750
/label="SV40_promoter"
rep_origin 2649..2726
/label="SV40_origin"
misc_feature 2711..2730
/label="SV40pro_F_primer"
CDS 2833..3627
/label="ORF frame 1"
gene 2836..3624
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(3142..3678)
/label="ORF frame 3"
terminator 3802..4071
/label="TK_PA_terminator"
rep_origin 4219..4838
/label="pBR322_origin"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctgccgcc accatggctg aaggatccgt cgccaggcag cctgacctct
661 tgacctgcga cgatgagccg atccatatcc ccggtgccat ccaaccgcat ggactgctgc
721 tcgccctcgc cgccgacatg acgatcgttg ccggcagcga caaccttccc gaactcaccg
781 gactggcgat cggcgccctg atcggccgct ctgcggccga tgtcttcgac tcggagacgc
841 acaaccgtct gacgatcgcc ttggccgagc ccggggcggc cgtcggagca ccgatcactg
901 tcggcttcac gatgcgaaag gacgcaggct tcatcggctc ctggcatcgc catgatcagc
961 tcatcttcct cgagctcgag cctccccagc gggacgtcgc cgagccgcag gcgttcttcc
1021 gccgcaccaa cagcgccatc cgccgcctgc aggccgccga aaccttggaa agcgcctgcg
1081 ccgccgcggc gcaagaggtg cggaagatta ccggcttcga tcgggtgatg atctatcgct
1141 tcgcctccga cttcagcggc gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa
1201 aactaggcct gcactatcct gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca
1261 tcaacccggt acggatcatt cccgatatca attatcggcc ggtgccggtc accccagacc
1321 tcaatccggt caccgggcgg ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc
1381 ccgtccatct ggaattcatg cgcaacatag gcatgcacgg cacgatgtcg atctcgattt
1441 tgcgcggcga gcgactgtgg ggattgatcg tttgccatca ccgaacgccg tactacgtcg
1501 atctcgatgg ccgccaagcc tgcgagctag tcgcccaggt tctggcctgg cagatcggcg
1561 tgatggaaga gtgagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
1621 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
1681 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
1741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
1801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
1861 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
1921 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
1981 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
2041 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
2101 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
2161 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
2221 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
2281 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
2341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
2401 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
2461 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
2521 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
2641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
2701 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
2761 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
2821 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
2881 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
2941 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
3001 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
3061 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
3121 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3181 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3241 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3301 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3361 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3421 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3481 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3541 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
3601 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
3661 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
3721 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
3781 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
3841 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
3901 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
3961 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
4021 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
4081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
4141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
4201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
4261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
4321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
4381 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
4441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
4501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
4561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
4621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
4681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
4741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
4801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
4861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
4921 gtattaccgc catgcat
//
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