| 出品公司: | -- |
|---|---|
| 载体名称: | p53-AbAi |
| 质粒类型: | 酵母单杂交系统 |
| 高拷贝/低拷贝: | 低拷贝 |
| 复制子: | pUC ori |
| 启动子: | URA3 |
| 载体大小: | 4944bp |
| 5' 测序引物及序列: | pABAI-F (GTTCCTTATATGTAGCTTTCGACA) |
| 3' 测序引物及序列: | pABAI-R (CCATCTCGAAAAAGGGTTTGCC) |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
p53-AbAi载体 p53AbAi质粒基本信息
p53-AbAi质粒图谱 p53AbAi载体图谱和p53-AbAi载体序列 p53AbAi质粒序列多克隆位点信息
p53-AbAi质粒 p53AbAi载体简介
p53-AbAi质粒序列 p53AbAi载体序列
LOCUS p53-AbAi 4944 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS p53-AbAi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4944)
TITLE Direct Submission
JOURNAL Exported Saturday, September 10, 2016
COMMENT Created by Clontech Laboratories Inc. http://www.biofeng.com/
Cat. No. 630491
FEATURES Location/Qualifiers
source 1..4944
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 25..83
/note="p53 binding site"
misc_feature 235..1440
/note="Aur1-C"
/note="Aureobasidin Resistance"
rep_origin 2209..2659
/note="pUC origin"
misc_feature complement(2856..3716)
/note="Amp(R)"
misc_feature complement(3925..4727)
/note="URA3"
ORIGIN
1 aagcttgaat tcgagctcgg taccaggcat gcctagcatg cctaggcatg cctaggcatg
61 cctaggcatg cctaggcatg cctggatccc tcgaggcatg tgctctgtat gtatataaaa
121 ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc tttgtagcat
181 aaattactat acttctatag acacgcaaac acaaatacac acactaaatt aataatggca
241 aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt agccgattta
301 gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa acccgcttta
361 agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt cattactaat
421 cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt attcatcatt
481 ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt ggcgctgtat
541 ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa agtgttacca
601 gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac atcgacgaat
661 tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg ggccccattt
721 gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg ttatgctttt
781 gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc agccgctccc
841 ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca tggctcgcct
901 ggtggattag ctagaattga taagctactc ggtattaata tgtatactac atgtttttca
961 aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc tactatggaa
1021 gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc ttatgtttgc
1081 tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct tatggcaggt
1141 tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc aattgtagat
1201 acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat atcaaagagt
1261 gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa cttggaactt
1321 gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt tgatggatct
1381 acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa gagggcttaa
1441 ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt
1501 tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca
1561 tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc
1621 tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac tctagcgaat
1681 taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
1741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
1801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
1861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
1921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
1981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
2041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
2101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
2161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
2221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
2281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
2341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
2401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
2461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
2521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
2581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
2641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
2701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
2761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
2821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
2881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
2941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
3001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
3061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
3121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
3181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
3241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
3301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
3361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
3421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
3481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
3541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
3601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
3661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
3721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
3781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
3841 ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
3901 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
3961 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag
4021 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata
4081 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca
//
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