pLZ271

LOCUS       pLZ271              8027 bp DNA     circular SYN 13-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..189
                     /label=182-WKG-596-F
     misc_feature    230
                     /label=CMV
     promoter        243..439
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    471..533
                     /label=Igk secretion signal
     regulatory      546..555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             554
                     /codon_start=1
                     /label=5xGS Linker
                     /translation=""
     CDS             555..617
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             618..692
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             693..755
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             756..830
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             837..899
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             900..974
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     CDS             975..1037
                     /codon_start=1
                     /label=ANR
                     /translation="GELDELVYLLDGPGYDPIHSD"
     CDS             1038..1112
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     misc_feature    1119..1868
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1120..1125
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1192..1214)
                     /label=186-WKG-603-R
     misc_feature    1398..1400
                     /label=F93A
                     /note="F93A"
     misc_feature    1846..1868
                     /label=EpoR-D1_D2_TM
                     /note="EpoR-D1_D2_TM"
     misc_feature    1846..1868
                     /label=transmembrane
                     /note="transmembrane"
     misc_feature    1869..3581
                     /label=VEGFR
                     /note="VEGFR"
     polyA_signal    3622..3846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3892..4320
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4334..4663
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4730..5524
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5698..5819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5864..5884)
                     /label=M13-rev
     primer_bind     complement(5868..5884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5890..5912)
                     /label=LacO
     protein_bind    5892..5908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5916..5946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5917..5946)
                     /label=lac
     protein_bind    5961..5982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6088..7028
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7029..7889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7890..7994)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tgatctgcga
      241 tctgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatcgctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctacgaat cgcgctagtg gccggccacc atggagaccg
      481 ataccttatt actgtgggtg cttcttctgt gggttcccgg tagcaccgga gacggatcca
      541 agcttgccac catgggggag ctcgacgagt tggtttatct cttagacgga cccggatatg
      601 accccattca ttcagatggc tccggaacgg gcagcgggac cggaagtggc accggaacca
      661 cttccggaac cggtactggc gggagtaccg gcggtgagct agacgaactc gtgtacctgt
      721 tggatggccc cggttacgac ccaatccaca gcgatggaag tggaactggg tctgggactg
      781 ggtccggcac cgggactact tcaggcaccg gcactggggg ctccactggc cataccgggg
      841 agctcgacga gttggtttat ctcttagacg gacccggata tgaccccatt cattcagatg
      901 gctccggaac gggcagcggg accggaagtg gcaccggaac cacttccgga accggtactg
      961 gcgggagtac cggcggtgag ctagacgaac tcgtgtacct gttggatggc cccggttacg
     1021 acccaatcca cagcgatgga agtggaactg ggtctgggac tgggtccggc accgggacta
     1081 cttcaggcac cggcactggg ggctccactg gccatatgga attcgcacct tcacccagcc
     1141 tcccggaccc caagtttgag agcaaagcgg ccctgctggc atcccggggc tccgaagaac
     1201 ttctgtgctt cacccaacgc ttggaagact tggtgtgttt ctgggaggaa gcggcgagct
     1261 ccgggatgga cttcaactac agcttctcat accagctcga gggtgagtca cgaaagtcat
     1321 gtagcctgca ccaggctccc accgtccgcg gctccgtgcg tttctggtgt tcactgccaa
     1381 cagcggacac atcgagtgct gtgccgctgg agctgcaggt gacggaggcg tccggttctc
     1441 ctcgctatca ccgcatcatc catatcaatg aagtagtgct cctggacgcc cccgcggggc
     1501 tgctggcgcg ccgggcagaa gagggcagcc acgtggtgct gcgctggctg ccacctcctg
     1561 gagcacctat gaccacccac atccgatatg aagtggacgt gtcggcaggc aaccgggcag
     1621 gagggacaca aagggtggag gtcctggaag gccgcactga gtgtgttctg agcaacctgc
     1681 ggggcgggac gcgctacacc ttcgctgttc gagcgcgcat ggccgagccg agcttcagcg
     1741 gattctggag tgcctggtct gagcccgcgt cactactgac cgctagcgac ctggaccctc
     1801 tcatcttgac gctgtctctc attctggtcc tcatctcgtt gttgctgacg gttctggccc
     1861 tgctgtccct acggaccgtt aagcgggcca atggagggga actgaagaca ggctacttgt
     1921 ccatcgtcat ggacccagat gaactcccat tggatgaaca ttgtgaacga ctgccttatg
     1981 atgccagcaa atgggagttc cccagagacc ggctgaagct aggtaagcct cttggccgtg
     2041 gtgcctttgg ccaagtgatt gaagcagatg cctttggaat tgacaagaca gcaacttgca
     2101 ggacagtagc agtcaaaatg ttgaaagaag gagcaacaca cagtgagcat cgagctctca
     2161 tgtctgaact caagatcctc attcatattg gtcaccatct caatgtggtc aaccttctag
     2221 gtgcctgtac caagccagga gggccactca tggtgattgt ggagttctgc aaatttggaa
     2281 acctgtccac ttacctgagg agcaagagaa atgaatttgt cccctacaag accaaagggg
     2341 cacgattccg tcaagggaaa gactacgttg gagcaatccc tgtggatctg aaacggcgct
     2401 tggacagcat caccagtagc cagagctcag ccagctctgg atttgtggag gagaagtccc
     2461 tcagtgatgt agaagaagag gaagctcctg aagatctgta taaggacttc ctgaccttgg
     2521 agcatctcat ctgttacagc ttccaagtgg ctaagggcat ggagttcttg gcatcgcgaa
     2581 agtgtatcca cagggacctg gcggcacgaa atatcctctt atcggagaag aacgtggtta
     2641 aaatctgtga ctttggcttg gcccgggata tttataaaga tccagattat gtcagaaaag
     2701 gagatgctcg cctccctttg aaatggatgg ccccagaaac aatttttgac agagtgtaca
     2761 caatccagag tgacgtctgg tcttttggtg ttttgctgtg ggaaatattt tccttaggtg
     2821 cttctccata tcctggggta aagattgatg aagaattttg taggcgattg aaagaaggaa
     2881 ctagaatgag ggcccctgat tatactacac cagaaatgta ccagaccatg ctggactgct
     2941 ggcacgggga gcccagtcag agacccacgt tttcagagtt ggtggaacat ttgggaaatc
     3001 tcttgcaagc taatgctcag caggatggca aagactacat tgttcttccg atatcagaga
     3061 ctttgagcat ggaagaggat tctggactct ctctgcctac ctcacctgtt tcctgtatgg
     3121 aggaggagga agtatgtgac cccaaattcc attatgacaa cacagcagga atcagtcagt
     3181 atctgcagaa cagtaagcga aagagccggc ctgtgagtgt aaaaacattt gaagatatcc
     3241 cgttagaaga accagaagta aaagtaatcc cagatgacaa ccagacggac agtggtatgg
     3301 ttcttgcctc agaagagctg aaaactttgg aagacagaac caaattatct ccatcttttg
     3361 gtggaatggt gcccagcaaa agcagggagt ctgtggcatc tgaaggctca aaccagacaa
     3421 gcggctacca gtccggatat cactccgatg acacagacac caccgtgtac tccagtgagg
     3481 aagcagaact tttaaagctg atagagattg gagtgcaaac cggtagcaca gcccagattc
     3541 tccagcctga ctcggggacc acactgagct ctcctcctgt ttaatctaga gggcccgttt
     3601 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3781 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3841 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4321 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4681 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4741 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4801 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4861 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4921 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4981 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5041 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5101 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5161 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5221 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5281 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5341 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5401 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5461 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5521 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5581 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5641 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5821 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcga
//