| 质粒名称: | pLYL95 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000598 |
| 产品用途: | Mammalian VanR-specific 8xANR-NSP3 expression vector (VanO5-Pmin-(ANR)8-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-Pmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (ANR)8-NSP3 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL95
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL95载体质粒基本信息
pLYL95质粒图谱载体图谱和pLYL95载体序列质粒序列多克隆位点信息
pLYL95质粒载体简介
pLYL95质粒序列载体序列
LOCUS pLYL95 6958 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6958)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6958
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 166..191
/label=182-WKG-596-F
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
promoter 324..355
/label=P_min
misc_feature 357..361
/label=NheI
misc_feature 372
/label=New Feature
misc_feature 372
/label=New Feature
regulatory 378..387
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 386
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 387..449
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 450..524
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 525..587
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 588..662
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 669..731
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 732..806
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 807..869
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 870..944
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 951..1013
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1014..1088
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1089..1151
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1152..1226
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1233..1295
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1296..1370
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1371..1433
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1434..1508
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1515..2456
/label=NSP3
misc_feature 1515..1538
/label=NSP3
primer_bind complement(1526..1545)
/label=179-WKG-592-R
misc_feature 2479..2484
/label=EcoRI
misc_feature 2518..2523
/label=New Feature
misc_feature 2518..2523
/label=New Feature
polyA_signal 2555..2779
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2825..3253
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3267..3596
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3663..4457
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4631..4752
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4797..4817)
/label=M13-rev
primer_bind complement(4801..4817)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4823..4845)
/label=LacO
protein_bind 4825..4841
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4849..4879)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4850..4879)
/label=lac
protein_bind 4894..4915
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5021..5961
/label=ColE1
/note="ColE1"
CDS complement(5962..6822)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6823..6927)
/gene="bla"
/label=AmpR promoter
promoter complement(6864..6892)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tttaattaag cagctcctgc aggtagaggg tatataatgg aagctcgact tccaggctag
361 cgtttaaact taagcttgcc accatggggg agctcgacga gttggtttat ctcttagacg
421 gacccggata tgaccccatt cattcagatg gctccggaac gggcagcggg accggaagtg
481 gcaccggaac cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac
541 tcgtgtacct gttggatggc cccggttacg acccaatcca cagcgatgga agtggaactg
601 ggtctgggac tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg
661 gccataccgg ggagctcgac gagttggttt atctcttaga cggacccgga tatgacccca
721 ttcattcaga tggctccgga acgggcagcg ggaccggaag tggcaccgga accacttccg
781 gaaccggtac tggcgggagt accggcggtg agctagacga actcgtgtac ctgttggatg
841 gccccggtta cgacccaatc cacagcgatg gaagtggaac tgggtctggg actgggtccg
901 gcaccgggac tacttcaggc accggcactg ggggctccac tggccatacc ggggagctcg
961 acgagttggt ttatctctta gacggacccg gatatgaccc cattcattca gatggctccg
1021 gaacgggcag cgggaccgga agtggcaccg gaaccacttc cggaaccggt actggcggga
1081 gtaccggcgg tgagctagac gaactcgtgt acctgttgga tggccccggt tacgacccaa
1141 tccacagcga tggaagtgga actgggtctg ggactgggtc cggcaccggg actacttcag
1201 gcaccggcac tgggggctcc actggccata ccggggagct cgacgagttg gtttatctct
1261 tagacggacc cggatatgac cccattcatt cagatggctc cggaacgggc agcgggaccg
1321 gaagtggcac cggaaccact tccggaaccg gtactggcgg gagtaccggc ggtgagctag
1381 acgaactcgt gtacctgttg gatggccccg gttacgaccc aatccacagc gatggaagtg
1441 gaactgggtc tgggactggg tccggcaccg ggactacttc aggcaccggc actgggggct
1501 ccactggcca tatgatgctc aagatggagt ctacacagca gatggctagt tcgattatta
1561 acacttcttt tgaagccgca gtcgttgctg ccacttcaac tcttgaactt atgggcattc
1621 aatatgatta taatgaagtt tatactagag ttaagagcaa atttgattac gtaatggatg
1681 attcaggtgt taaaaacaat ctgttgggta aagcggcaac tattgatcag gcactgaatg
1741 gaaaatttgg ttcagcagca agaaatagaa attggatggc tgatactaga actacagcta
1801 gactagatga agatgtgaac aaacttagaa tgatgctatc atctaaagga attgatcaaa
1861 aaatgagagt acttaatgca tgtttcaatg tgaagcgagt tccaggtaaa tcatcatcaa
1921 taattaaatg tactagatta atgagagata agattgaacg tggtgaagtc gaagtggatg
1981 attcgtttgt tgaagaaaaa atggaagttg acacaattga ttggaaatct agatatgaac
2041 aattggaaaa acgatttgaa tcgctgaaac aaagggtgaa tgaaaaatat acaagttggg
2101 tgcaaaaagc aaaaaaggta aatgaaaata tgtactctct ccaaaacgtt atatcacaac
2161 agcagagcca gattgctgat ttacaaaatt actgtaataa attggaagtt gatctacaaa
2221 ataaaattag ttcacttgtg tcttcagtcg aatggtacct gaaatcaatg gaattgcctg
2281 atgaaattaa gacagatatt gaacaacaac taaattccat tgatgtaatc aatccaatta
2341 atgctataga tgattttgaa tcactgatca gaaatattat actcgactat gacagaatat
2401 ttttaatgtt taaaggatta atgaggcaat gcaactatga atatacgtat gaataggatc
2461 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
2521 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
2581 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
2641 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
2701 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
2761 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
2821 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
2941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
3001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
3061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
3121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
3181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
3241 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
3301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
3421 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3481 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
3541 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3601 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
3661 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3721 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3781 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3841 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3901 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3961 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
4021 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
4081 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
4141 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
4201 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
4261 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
4321 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
4381 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
4441 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
4501 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
4561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
4621 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
4681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
4741 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
4801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
5041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
5101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
5161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
5221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
5281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
5341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
5401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
5461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
5521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
5581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
5641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
5701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
5761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
5821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
5881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
5941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
6001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
6061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
6121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
6181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
6241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
6301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
6361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
6421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
6481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
6541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
6601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
6661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
6721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
6781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
6841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
6901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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