| 质粒名称: | vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 160044 |
| 存储实验室: | Michael Elowitz |
|
载体骨架基本信息 |
|
| 载体名称: | PiggyBac |
| 载体出品公司: | SYSTEM BIOSCIENCE |
| 载体类型: | 哺乳动物表达，合成生物学 |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | iaaH |
| 启动子: | pEF1s |
| 标签/融合蛋白: | 3xNLS Citrine（插入件上的N端子） |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | TGGAGTAGCCATCACGTCACTGGC |
| 3’测序引物 | GGTGTTCGGAAGTCCAGCGAAAT |
|
文献引用方法 |
|
| 材料和方法部分: | vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro) was a gift from Michael Elowitz (Addgene plasmid # 160044 ; http://n2t.net/addgene:160044 ; RRID:Addgene_160044) |
| 参考文献部分: | Synthetic mammalian signaling circuits for robust cell population control. Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB. Cell. 2022 Mar 17;185(6):967-979.e12. doi: 10.1016/j.cell.2022.01.026. Epub 2022 Mar 1. 10.1016/j.cell.2022.01.026 PubMed 35235768 |
vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-3xNLS-Citrine-T2A-iaaH(Hygro)载体图谱.png)
载体简介
载体序列
LOCUS vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro) 9390 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION For expressing iaaH to convert auxin precursors (IAM or NAM) to
auxin (IAA or NAA).
ACCESSION .
VERSION .
KEYWORDS vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9390)
TITLE Synthetic mammalian signaling circuits for robust cell population
control
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9390)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9390
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..269
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 306..326
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 330..350
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 354..374
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 390..1103
/codon_start=1
/product="enhanced variant of YFP (Heikal et al., 2001)"
/label=Citrine
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(432..453)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(693..712)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1040..1061
/label=EGFP-C
/note="EGFP, forward primer"
CDS 1104..1157
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
primer_bind complement(2582..2599)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2588..2795
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(2828..2848)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2833..3162
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3013..3148
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3075..3094
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3211..4236
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(4296..4316)
/label=WPRE-R
/note="WPRE, reverse primer"
polyA_signal 4358..4479
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4395..4414)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4449..4468
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
repeat_region 5242..5276
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(5767..5783)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5767..5783)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5780..5802)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5791..5807
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5815..5845)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5860..5881
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5998..6015)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6169..6757)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6249..6268)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6928..7788)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7551..7570
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7789..7893)
/gene="bla"
/label=AmpR promoter
rep_origin complement(7919..8374)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8056..8077)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8268..8287
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8501..8523
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8515..8532
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8516..8532
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
repeat_region 8662..8724
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
ORIGIN
1 ggggctagca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacaccgg
61 ttgagttgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgtgtc cgccgtctag
121 gtaagtttaa agctcaggtt gagaccgggc ctttgtccgg tgctcccttg gagcctacct
181 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
241 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac ctgggcctac ggatccgcca
301 ccatgcccaa aaaaaagcga aaagtgggtc ctaagaagaa acggaaggtt ggccccaaaa
361 agaaacggaa ggtcggtagt ggtggcagtg tgagcaaggg cgaggagctg ttcaccgggg
421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
541 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
601 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
961 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
1021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
1081 tcggcatgga cgagctgtac aaggagggca gggggagcct cctaacttgc ggagatgtcg
1141 aggagaatcc tggcccaggt ggagtagcca tcacgtcact ggcccaatct ttggagcatc
1201 ttaaacgaaa agactattct tgtcttgaat tggttgagac acttattgcc agatgcgagg
1261 ccgcgaaaag tcttaatgct cttctcgcaa cggattggga cgggttgagg agatccgcta
1321 agaagataga ccggcatgga aatgctgggg ttggactctg tgggataccg ctgtgtttca
1381 aggcaaacat cgccacgggt gtgttcccca cgagtgcggc cacgccagcc ttgattaacc
1441 accttcctaa aatcccatcc cgcgtagcag aaaggctgtt ttccgcaggc gctcttccag
1501 gggcttccgg aaatatgcac gagcttagtt ttggcattac tagcaacaac tatgctactg
1561 gggccgtgcg caacccatgg aacccagacc tcattccggg tgggagttct ggcggtgtcg
1621 ccgccgcggt agcgtcacgc ttgatgctgg ggggaatcgg aaccgacaca ggggccagcg
1681 taaggttgcc agcagccctg tgtggcgtcg taggcttccg acctacactc ggacgctacc
1741 cgggcgaccg cattatccct gttagtccca ctcgcgatac tcctggtatt atcgctcaat
1801 gtgtggctga cgtcgtcatc ctggatcgga tcataagtgg tacaccagaa cgaatcccac
1861 cggttccgct taaaggcctc cggattgggc tgcccacgac ctatttttat gatgacctcg
1921 atgccgatgt cgccttggcg gcggagacta cgattagact tcttgcgaac aaaggggtca
1981 cgtttgttga ggctaacata ccgcacttgg acgagttgaa taaaggggcg tcttttcccg
2041 tggcactgta tgaatttccg cacgctctta aacagtacct ggacgatttc gttaaaacgg
2101 ttagcttttc agatgtaata aagggcatcc gatcaccaga tgtggctaat atagctaatg
2161 cccaaattga cggtcatcaa atttccaaag ccgagtatga actcgcccgc cacagctttc
2221 ggccaagact tcaggcgaca tatcgaaatt actttaagct taatcgcttg gatgcgatac
2281 ttttccccac cgcccctctg gtagccagac cgattggtca agactcttca gttatacaca
2341 atggaactat gcttgataca tttaagatat atgtacggaa cgtagaccca agctcaaatg
2401 cagggctccc gggcttgtca attccagttt gccttacgcc ggaccggctt ccagtgggga
2461 tggaaataga tggacttgcg gactctgacc aacgccttct cgctataggc ggtgccctgg
2521 aagaagcgat tgggtttaga tatttcgctg gacttccgaa cacctagcga atttaaatcg
2581 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
2641 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
2701 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
2761 gaggattggg aagacaatag caggcatgct ggggacgcgg ccgcaattta acgcgaatta
2821 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
2881 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
2941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3001 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3061 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
3121 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
3181 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
3241 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
3301 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
3361 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
3421 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
3481 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
3541 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
3601 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
3661 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
3721 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
3781 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
3841 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
3901 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
3961 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
4021 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
4081 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
4141 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
4201 cgacgcccca gcactcgtcc gagggcaaag gaataggtcg acaatcaacc tctggattac
4261 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
4321 tacgctgctt taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa
4381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
4441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
4501 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
4561 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
4621 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
4681 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
4741 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
4801 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
4861 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
4921 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
4981 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
5041 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
5101 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
5161 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
5221 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
5281 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
5341 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
5401 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
5461 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
5521 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
5581 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
5641 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
5701 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
5761 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
5821 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
5881 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
5941 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
6001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
6061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
6121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
6181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
6241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
6301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
6361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
6421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
6481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
6541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
6601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
6661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
6721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
6781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
6841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
6901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
6961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
7021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
7081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
7141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
7201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
7261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
7321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
7381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
7441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
7501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
7561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
7621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
7681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
7741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
7801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
7861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
7921 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
7981 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
8041 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
8101 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
8161 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
8221 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
8281 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
8341 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
8401 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
8461 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
8521 acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag
8581 actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
8641 cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat
8701 aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta
8761 atttgaatag atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca
8821 aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat
8881 aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac
8941 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
9001 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
9061 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
9121 tacggggaaa aggcctggat ctgccattgc tccagtgccc ttcagtgggc agagagcaca
9181 tcacccacag tccccaagaa gttgggggga ggggtgggca attgaactgg tgcctagaga
9241 aggtggcgca gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
9301 ggtgggggag aaccatatat aagtgcagta gtccccctga acgttctttt ttgcaacggg
9361 tttgccgcca gaacacagct gaagctttga
//
