pDONR221

价格:800元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pDONR221
质粒类型: Gateway载体;克隆载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: Gateway ; BP反应
载体大小: 4762 bp
5' 测序引物及序列: M13-F(-20): GTAAAACGACGGCCAG
3' 测序引物及序列: M13-R: CAGGAAACAGCTATGAC
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素/ 氯霉素
筛选标记: --
备注:
pDONR221载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
 
产品目录号: 12536-017
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDONR221载体图谱



pDONR221多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       pDONR221                4762 bp    DNA     circular      2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pDONR221|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     source          1..4762
                     /invitrogen="722"
                     /vntifkey="98"
     CDS             complement(1197..1502)
                     /invitrogen="1060000"
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(1847..2506)
                     /invitrogen="1080000"
                     /vntifkey="4"
                     /label=Cm(R)
     CDS             3156..3965
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     primer          537..552
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     primer          517..533
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          complement(3027..3043)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          complement(3003..3022)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        complement(3006..3022)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      427..470
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      268..295
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     misc_recomb     570..801
                     /invitrogen="2685000"
                     /vntifkey="86"
                     /label=attP1
     misc_recomb     complement(2754..2985)
                     /invitrogen="2686000"
                     /vntifkey="86"
                     /label=attP2
BASE COUNT     1260 a      1121 c      1060 g      1321 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 
      661 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 
      721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt 
      781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct 
      841 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca 
      901 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg 
      961 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt 
     1021 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta 
     1081 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt 
     1141 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat 
     1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca 
     1261 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc 
     1321 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc 
     1381 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc 
     1441 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc 
     1501 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac 
     1561 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc 
     1621 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac 
     1681 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg 
     1741 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat 
     1801 acgcatactg ttatctggct tttagtaagc cggatccacg cggcgtttac gccccgccct 
     1861 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 
     1921 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 
     1981 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 
     2041 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 
     2101 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 
     2161 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 
     2221 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 
     2281 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 
     2341 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 
     2401 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 
     2461 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 
     2521 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 
     2581 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg 
     2641 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca 
     2701 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt 
     2761 cactaatacc atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt 
     2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 
     2881 tttctcgttc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt 
     2941 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata 
     3001 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt 
     3061 ctcaaaatct ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac 
     3121 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 
     3181 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 
     3241 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 
     3301 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 
     3361 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 
     3421 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 
     3481 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 
     3541 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 
     3601 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 
     3661 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 
     3721 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 
     3781 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 
     3841 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 
     3901 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 
     3961 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 
     4021 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 
     4081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     4501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     4741 gctggccttt tgctcacatg tt 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

  • 上一篇:没有了
  • 下一篇:pENTR3C
分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng