pShuttle

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载体基本信息

出品公司: Agilent
载体名称: pShuttle
GenBank序列号: AF334399
质粒类型: 腺病毒表达载体,哺乳动物细胞表达载体
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6568 bp
5' 测序引物及序列: pShuttle-F: GAAGTGAAATCTGAATAATTTTGTG
3' 测序引物及序列: pShuttle-R: CAAAACTACATAAGACCCCCAC
载体标签: --
载体抗性: 卡那
备注: --
产品目录号: 240006
稳定性: /
组成型: --
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pShuttle载体图谱


pShuttle多克隆位点


pShuttle载体特征

载体简介

pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.

载体序列

LOCUS       pShuttle           6568 bp    DNA     circular SYN 07-FEB-2001
DEFINITION  AdEasy vector pShuttle, complete sequence.
ACCESSION   AF334399
VERSION     AF334399.1  GI:12698892
KEYWORDS    .
SOURCE      AdEasy vector pShuttle
  ORGANISM  AdEasy vector pShuttle
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Grafsky,A.J. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2001) Technical Services, Stratagene, 11011 N.
            Torrey Pines Rd, La Jolla, CA 92037, USA
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="AdEasy vector pShuttle"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:148367"
                     /lab_host="Escherichia coli"
                     /label="extrachromosomal
                     used for the cloning of cDNA to be recombined with the
                     pADEasy-1 vector; for infection of, and expression in,
                     various mammalian cell types"
     LTR             4..106
                     /label="left inverted terminal repeat (LITR)"
     misc_signal     197..345
                     /label="encapsidation"
     primer_bind     313..337
                     /label="forward"
     misc_feature    359..418
                     /label="MCS"
     misc_feature    416..2414
                     /label="right arm homology region"
     primer_bind     452..473
                     /label="reverse"
     misc_feature    2682..3583
                     /label="left arm homology region"
     LTR             3584..3686
                     /label="right inverted terminal repeat (RITR)"
     rep_origin      3764..4542
                     /label="pBR322 ori"
     CDS             5289..6083
                     /label="kanamycin resistance protein"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAK01721.1"
                     /db_xref="GI:12698893"                     
ORIGIN      
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat actggtaccg cggccgcctc gagtctagag atatcgaatt
      361 caagcttgtc gactcgaaga tctgggcgtg gttaagggtg ggaaagaata tataaggtgg
      421 gggtcttatg tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc
      481 gtttgatgga agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt
      541 gcgtcagaat gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac
      601 taccttgacc tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc
      661 ttcagccgct gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct
      721 tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc
      781 acaattggat tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg
      841 ccagcaggtt tctgccctga aggcttcctc ccctcccaat gcggtttaaa acataaataa
      901 aaaaccagac tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt atttaggggt
      961 tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt
     1021 ttccaggacg tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct
     1081 ggggtggagg tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca
     1141 gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc
     1201 caggggcagg cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg
     1261 tggggatatg agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc
     1321 cctccgggga ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa
     1381 tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc
     1441 aagattttcc atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc
     1501 gaagatattt ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc
     1561 catttttaca aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc
     1621 aggggcgtag ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat
     1681 catgtctacc tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga
     1741 agaaagcagg ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc
     1801 tattaccggg tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg
     1861 ggccacttcg ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag
     1921 gcgctcgccg cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag
     1981 accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag
     2041 ctcggtcacc tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg
     2101 cggctttcgc tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc
     2161 cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc
     2221 tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct
     2281 tcgccctgcg cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg
     2341 gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga
     2401 cttttgaggg cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg
     2461 ccgcaggccc cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg
     2521 tcaaaaacca ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc
     2581 cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga ctnnngttta
     2641 aacgaattcn nntataaaat gcaaggtgct gctcaaaaaa tcaggcaaag cctcgcgcaa
     2701 aaaagaaagc acatcgtagt catgctcatg cagataaagg caggtaagct ccggaaccac
     2761 cacagaaaaa gacaccattt ttctctcaaa catgtctgcg ggtttctgca taaacacaaa
     2821 ataaaataac aaaaaaacat ttaaacatta gaagcctgtc ttacaacagg aaaaacaacc
     2881 cttataagca taagacggac tacggccatg ccggcgtgac cgtaaaaaaa ctggtcaccg
     2941 tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg gagtcataat gtaagactcg
     3001 gtaaacacat caggttgatt catcggtcag tgctaaaaag cgaccgaaat agcccggggg
     3061 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
     3121 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
     3181 ctcccgctcc agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca
     3241 gtaaaaaaga aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac
     3301 agtgtaaaaa agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg
     3361 ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc
     3421 caaaaaaccc acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc
     3481 attttaagaa aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca
     3541 cccgccccgt tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg
     3601 gcttcaatcc aaaataaggt atattattga tgatnnntta attaaggatc cnnncggtgt
     3661 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     3721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4921 agttcgccag ttaatagttt gcgcaacgtt gttgnnnnnn aaaaaggatc ttcacctaga
     4981 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     5041 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     5101 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     5161 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     5221 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     5281 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5461 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     5821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6061 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
     6121 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
     6181 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6241 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
     6301 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
     6361 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6421 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     6481 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
     6541 taattaacat catcaataat atacctta
//

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