pUG66

价格:3800元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Hegemann Lab
载体名称: pUG66
质粒类型: 酵母菌株基因敲除载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3580 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: Bleomycin 博来霉素
产品目录号: --
稳定性: 稳定
组成型: 组成型表达
病毒/非病毒: 非病毒
备注: pUG66载体是大肠杆菌-酵母穿梭载体,在大肠杆菌中抗性是氨苄。pUG66载体质粒含有来自Tn5载体质粒的Ble基因,使得载体质粒在酵母菌中呈现出博来霉素抗性。

pUG66载体在筛选基因Ble两侧含有loxp基因,通过表达cre重组酶,可以利用loxp基因将Ble s筛选基因删除。

pUG66载体可以作为酵母基因敲除的一个质粒PCR模板。代表性的PCR片段的整合插入宿主菌,使得基因敲除突变子能够进行顺利筛选。

载体质粒图谱和多克隆位点信息

pUG66载体图谱


pUG66载体图谱

载体简介

载体序列

LOCUS       pUG66                3580 bp    DNA     circular SYN 11-MAR-2002
DEFINITION  PCR template vector pUG66, complete sequence.
ACCESSION   AF298794
VERSION     AF298794.1  GI:11344912
KEYWORDS    .
SOURCE      PCR template vector pUG66
  ORGANISM  PCR template vector pUG66
            other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
  TITLE     A second set of loxP marker cassettes for Cre-mediated multiple
            gene knockouts in budding yeast
  JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)
   PUBMED   11884642
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="PCR template vector pUG66"
                     /mol_type="other DNA"
                     /db_xref="taxon:142963"
                     /note="derived from Saccharomyces cerevisiae"
     misc_feature    54..87
                     /note="loxP site"
     regulatory      88..484
                     /regulatory_class="promoter"
                     /note="from TEF"
     gene            486..866
                     /gene="ble"
     CDS             486..866
                     /gene="ble"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="AAG34545.1"
                     /db_xref="GI:11344913"
     regulatory      873..1131
                     /regulatory_class="terminator"
                     /note="from TEF"
     misc_feature    1132..1165
                     /note="loxP site"
     gene            complement(2254..3114)
                     /gene="AmpR"
     CDS             complement(2254..3114)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance protein"
                     /protein_id="AAG34546.1"
                     /db_xref="GI:11344914"
ORIGIN      
        1 agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacaaccctt aatataactt
       61 cgtataatgt atgctatacg aagttattag gtctagagat ctgtttagct tgcctcgtcc
      121 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
      181 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
      241 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
      301 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
      361 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
      421 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
      481 caaccatggc cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccacggccg
      541 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
      601 agcgcgggga tctcaagctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt
      661 ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg
      721 tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt
      781 ggggaggcac gatggccgct ttggtcgacc cggacgggac gctcctgcgc ctgatacaga
      841 acgaattgct tgcaggcatc tcatgatcag tactgacaat aaaaagattc ttgttttcaa
      901 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
      961 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     1021 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     1081 atactaacgc cgccatccag tgtcgaaaac gagctctcga gaacccttaa tataacttcg
     1141 tataatgtat gctatacgaa gttattaggt gatatcagat ccactagtgg cctatgcggc
     1201 cgcggatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc aggttaacct
     1261 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1681 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3241 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3301 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3361 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3421 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     3481 tgtactgaga gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat
     3541 aaccttatgt atcatacaca tacgatttag gtgacactat
//

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