| 质粒名称: | R4pGWB601_UBQ10p-miniTurbo-YFP-NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 127370 |
| 存储实验室: | Dominique Bergmann |
|
载体骨架基本信息 |
|
| 载体名称: | R4pGWB601 |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 12680 bp |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | Basta |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | miniTurbo (BirA mutant) |
| 物种来源: | BirA from E. coli, codon optimized for human and mutated |
| 插入大小: | 771 bp |
| 基因突变: | aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V |
| GenBank编号 | -- |
| 启动子 | Arabidopsis UBQ10 promoter |
| 标签/融合蛋白: |
V5（插入N端子）
GS链接器（插入时为C终端）
YFP（插入件上的C端子）
NLS（插入的C端子）
|
| 克隆方法: | Gateway 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13 fw |
| 3' 测序引物 | M13 rv |
|
文献引用方法 |
|
| 材料和方法部分: | R4pGWB601_UBQ10p-miniTurbo-YFP-NLS was a gift from Dominique Bergmann (Addgene plasmid # 127370 ; http://n2t.net/addgene:127370 ; RRID:Addgene_127370) |
| 参考文献部分: | Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC. Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. 10.7554/eLife.47864 PubMed 31535972 |
买家导航
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS载体质粒基本信息
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS质粒图谱载体图谱和R4pGWB601_UBQ10p-miniTurbo-YFP-NLS载体序列质粒序列多克隆位点信息
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS质粒载体简介
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS质粒序列载体序列
LOCUS R4pGWB601_UBQ10p-miniTurbo-YFP-NLS 12680 bp ds-DNA circular SYN 07-JUN-2019
DEFINITION Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10
promoter in plants.
ACCESSION .
VERSION .
KEYWORDS R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12680)
TITLE Plasmids for proximity labeling with TurboID and miniTurbo
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12680)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12680
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 188..210
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 202..219
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 203..219
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 244..264
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 2283..2307
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2321..2362
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 3167..3883
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3212..3233)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3473..3492)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3820..3841
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3893..3913
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
protein_bind complement(3933..3957)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
terminator 3989..4241
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(4274..4290)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4274..4290)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4287..4309)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4298..4314
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4322..4352)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4367..4388
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
terminator 4424..4676
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4836..5387)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(5439..5618)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 6124..6148
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 6669..7460
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7706..8294
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8195..8214
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8448..8465
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8480..8620
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8546..8565)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8706..8728
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 8964..9158
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(9224..10297)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(10726..11355)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 12656..12680
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc
61 tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
121 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
181 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgt ggatccccca
241 tcacaacttt gtatagaaaa gttggctccg aattcgccct tagtctagct caacagagct
301 tttaacccaa attggtacaa tagaatacaa ctttagatca taattctcaa aagaaagaga
361 ttccttagct attctatctg ccactccatt tccttctcgg cttgtatgca caagcataaa
421 atcctcaaac ttgctaagta gatactttat gtcttggata attggattga gacttgacaa
481 gcataacttt catgtaacca aagacacaag ttgctgagaa tccacctcaa aaatgatctt
541 cctataattg aatcgggata atgacagcac agcccatcta agagcctcca cttctacttc
601 cagcacgctt cttactttta ccacagctct tgcacctaac cataacacct tccctgtatg
661 atcgcgaagc acccacccta agccacattt taatccttct gttggccatg ccccatcaaa
721 gttgcactta acccaagatt gtggtggagc ttcccatgtt tctcgtctgt cccgacggtg
781 ttgtggttgg tgctttcctt acattctgag cctctttcct tctaatccac tcatctgcat
841 cttcttgtgt ccttactaat acctcattgg ttccaaattc cctcccttta agcaccagct
901 cgtttctgtt cttccacagc ctcccaagta tccaagggac taaagcctcc acattcttca
961 gatcaggata ttcttgttta agatgttgaa ctctatggag gtttgtatga actgatgatc
1021 taggaccgga taagttccct tcttcatagc gaacttattc aaagaatgtt ttgtgtatca
1081 ttcttgttac attgttatta atgaaaaaat attattggtc attggactga acacgagtgt
1141 taaatatgga ccaggcccca aataagatcc attgatatat gaattaaata acaagaataa
1201 atcgagtcac caaaccactt gcctttttta acgagacttg ttcaccaact tgatacaaaa
1261 gtcattatcc tatgcaaatc aataatcata caaaaatatc caataacact aaaaaattaa
1321 aagaaatgga taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc
1381 actgatttta taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga
1441 aataaagcac gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg
1501 ttcaattatt gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa
1561 aaaaatataa atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttag
1621 aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa agtggttgca gccggcacac
1681 acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa
1741 ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag
1801 ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt
1861 cttctataaa acaataccca aagagctctt cttcttcaca attcagattt caatttctca
1921 aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc
1981 ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt
2041 ggtttagatt ctgttaatct tagatcgaag acgattttct gggtttgatc gttagatatc
2101 atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt
2161 ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt agtttctagt
2221 ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacagaag ggcgaattcg
2281 acccaagttt gtacaaaaaa gcaggctccg cggccgcatg ggcaagccca tccccaaccc
2341 cctgctgggc ctggacagca ccgctagcat cccgctgctg aacgctaaac agattctggg
2401 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
2461 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
2521 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
2581 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
2641 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
2701 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
2761 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
2821 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
2881 gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
2941 ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc
3001 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
3061 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag
3121 tctgcggtct gccgaaaagg gaggcggtgg aagcggcgga ggttccatgg tgagcaaggg
3181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
3241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
3301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
3361 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
3421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
3481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
3541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
3601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
3661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
3721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
3781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
3841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggcttcgg atccaaaaaa
3901 gaagagaaag gtatagaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttga
3961 taacagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
4021 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
4081 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
4141 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
4201 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc
4261 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
4321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
4381 tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag taacatagat
4441 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
4501 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
4561 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
4621 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
4681 aaattcgagc tggggacgtc cccagctcgg taccccctcc tgggcacggt cgtacgcgat
4741 ccgccgcggt tccacctcac cattgcgggt atgccagttg agttcgatga gttcccgtac
4801 cagctccaac tcgggcgatg ccacgcgcac tccgttcaga tctcggtgac gggcaggacc
4861 ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc
4921 ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc
4981 atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt
5041 gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg
5101 cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag
5161 gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag
5221 cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg
5281 aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc
5341 gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatatc ttattgcccc
5401 ccgggatcag atctggattg agagtgaata tgagactcta attggatacc gaggggaatt
5461 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
5521 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
5581 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
5641 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat
5701 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
5761 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
5821 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
5881 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
5941 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
6001 tgcttgcggc agcgtgaagc tgatccgtcg agattaatag aaataaattc agcctaattc
6061 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
6121 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
6181 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
6241 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
6301 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
6361 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
6421 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
6481 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
6541 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
6601 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
6661 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
6721 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
6781 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
6841 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
6901 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
6961 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
7021 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
7081 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
7141 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
7201 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
7261 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
7321 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
7381 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
7441 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
7501 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
7561 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
7621 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
7681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
8281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
8341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
8401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
8461 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
8521 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
8581 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
8641 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
8701 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
8761 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
8821 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
8881 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
8941 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
9001 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
9061 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
9121 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
9181 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
9241 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
9301 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
9361 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
9421 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
9481 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
9541 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
9601 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
9661 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
9721 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
9781 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
9841 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
9901 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
9961 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
10021 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
10081 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
10141 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
10201 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
10261 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
10321 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
10381 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
10441 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
10501 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
10561 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
10621 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
10681 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
10741 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
10801 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
10861 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
10921 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
10981 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
11041 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
11101 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
11161 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
11221 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
11281 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
11341 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
11401 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
11461 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
11521 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
11581 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
11641 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
11701 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
11761 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
11821 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
11881 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
11941 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
12001 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
12061 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
12121 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
12181 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
12241 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
12301 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
12361 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
12421 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
12481 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
12541 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
12601 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
12661 cccgccaata tatcctgtca
//
