| 出品公司: | -- |
|---|---|
| 载体名称: | pFUSE-hIgG1e1-Fc2 |
| 质粒类型: | 哺乳细胞，抗体表达载体 |
| 终止子: | SV40 poly(A) signal |
| 复制子: | pUC |
| 启动子: | EF1α |
| 载体大小: | 4194bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 博莱霉素 |
| 筛选标记: | 博莱霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFUSE-hIgG1e1-Fc2 4194 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4194)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..4194
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 31..242
/note="EF-1-alpha core promoter"
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 255..523
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 573..632
/codon_start=1
/product="human interleukin-2 signal sequence"
/note="IL-2 signal sequence"
/translation="MYRMQLLSCIALSLALVTNS"
polyA_signal 1359..1480
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
polyA_site 1584..1978
/note="beta-globin poly(A)"
/note="human beta-globin polyadenylation signal"
CDS complement(1992..2366)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
enhancer 2959..3262
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(3342..3930)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 4038..4086
/note="synthetic polyadenylation signal"
misc_feature 4097..4188
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctgacaaaa
661 ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct
721 tccccccaaa acccaaggac caactgatga tctcccggac ccctgaggtc acatgcgtgg
781 tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg
841 aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg
901 tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg
961 tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc
1021 cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg
1081 tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga
1141 gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct
1201 ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct
1261 tctcatgctc cgtgctgcat gaggctctgc acaaccacta cacgcagaag agcctctccc
1321 tgtctccggg taaatgagtg ctagctggcc agacatgata agatacattg atgagtttgg
1381 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
1441 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
1501 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
1561 caaatgtggt atggaattaa ttctaaaata cagcatagca aaactttaac ctccaaatca
1621 agcctctact tgaatccttt tctgagggat gaataaggca taggcatcag gggctgttgc
1681 caatgtgcat tagctgtttg cagcctcacc ttctttcatg gagtttaaga tatagtgtat
1741 tttcccaagg tttgaactag ctcttcattt ctttatgttt taaatgcact gacctcccac
1801 attccctttt tagtaaaata ttcagaaata atttaaatac atcattgcaa tgaaaataaa
1861 tgttttttat taggcagaat ccagatgctc aaggcccttc ataatatccc ccagtttagt
1921 agttggactt agggaacaaa ggaaccttta atagaaattg gacagcaaga aagcgagctt
1981 ctagcttatc ctcagtcctg ctcctctgcc acaaagtgca cgcagttgcc ggccgggtcg
2041 cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag
2101 gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg
2161 cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg
2221 aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag
2281 aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc
2341 ggaacggcac tggtcaactt ggccatgatg gctcctcctg tcaggagagg aaagagaaga
2401 aggttagtac aattgctata gtgagttgta ttatactatg cagatatact atgccaatga
2461 ttaattgtca aactagggct gcagggttca tagtgccact tttcctgcac tgccccatct
2521 cctgcccacc ctttcccagg catagacagt cagtgactta ccaaactcac aggagggaga
2581 aggcagaagc ttgagacaga cccgcgggac cgccgaactg cgaggggacg tggctagggc
2641 ggcttctttt atggtgcgcc ggccctcgga ggcagggcgc tcggggaggc ctagcggcca
2701 atctgcggtg gcaggaggcg gggccgaagg ccgtgcctga ccaatccgga gcacatagga
2761 gtctcagccc cccgccccaa agcaagggga agtcacgcgc ctgtagcgcc agcgtgttgt
2821 gaaatggggg cttggggggg ttggggccct gactagtcaa aacaaactcc cattgacgtc
2881 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
2941 ctgccaaaac cgcatcatca tggtaatagc gatgactaat acgtagatgt actgccaagt
3001 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
3061 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
3121 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
3181 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
3241 atttaccgta agttatgtaa cgcctgcagg ttaattaaga acatgtgagc aaaaggccag
3301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
3361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
3421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
3481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
3541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
3601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
3661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
3721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
3781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
3841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
3901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
3961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatggctag ttaattaaca
4021 tttaaatcag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
4081 tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
4141 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaa
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pNL1.3[secNluc]
- 下一篇：pAAV-tre-mcherry
