| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001673 |
| 产品用途: | 3' AAVLINK plasmid for COL1A2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG 7148 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7148)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7148
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3861
/codon_start=1
/label=COL1A2
/translation="VRVVLPVLLVLLEAEVLLDPQGLMETRVNLVWLVLWALLVHLVLV
DSQERGVLLAYLEAREKRVNLVSEVKLVTLAEMVLVVLLVL*VPLVLLEPQVTGAKLGL
LVLLVLLVLGEALVNVVRSVLLAPMDLLVLLVLLVNLVLKEKEEPKGLRVKTVLLVPQA
PLELLAQLVQMVPPVLLEVVVMEAPLV*LVSLVLLDGLVPQDPLVFLALLVPLVLLGKK
GFVVLVVTKVQLAELEK*VQLVPLASLVRRVPLERLVLLDLLALQVLRVFLVLLVFWVS
LAREVNVVYQVLLVLWVNLVLLALPALLGPVVLLVLWVVLESTVLLVKLVVMATLGTMV
PQVAMVNPDTRESAVTLAILVPLVLQVHLVLMAPWVLLANMETVVKLVLLVLLVLLVLL
AQEVLVAHKAFVAIRESPVKRGPEVFLA*RDTMDCKVCLVSLVTMVIKVLLAPWVLLVL
GALLVLLALLEKMVALDILVQLDLLAFEALRVTKALLAPLVPLALLDLQV*AVVVMTLV
TMETSTGLTSLAQHLLSDPRTMKLMLL*SLSTTRLRPFLLLKALERTQLAHAVT*DSAT
QSGAVVTTGLTLTKDALWMLSKYTVISLLAKPVSGPNLKTSQPRTGIGAPRTRNTSG*E
KLSMLAASLNIM*KE*LPRKWLPNLPSCACWPTMPLRTSPTTARTALHTWMRRLAT*KR
LSFYRALMMLNLLLRATAGSLTLFL*MAALKRQMNGERQSLNTKQISHHACPSLILHLW
TSVVLTRNSLWTLAQSVS"
misc_feature 2179..2184
/label=SDS
misc_feature 2332..2337
/label=SDS
misc_feature 2467..2472
/label=SDS
misc_feature 2485..2490
/label=SDS
misc_feature 3052..3057
/label=SDS
CDS 3868..3933
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3937..4368
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4081..4086
/label=SDS
repeat_region 4411..4551
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4411..4540
/label=AAV2 ITR
rep_origin 4626..5081
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5363..5467
/gene="bla"
/label=AmpR promoter
CDS 5468..6328
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6499..7087
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga gagtggtgct
1561 gccggtccta ctggtcctat tggaagccga ggtccttctg gacccccagg gcctgatgga
1621 aacaagggtg aacctggtgt ggttggtgct gtgggcactg ctggtccatc tggtcctagt
1681 ggactcccag gagagagggg tgctgctggc atacctggag gcaagggaga aaagggtgaa
1741 cctggtctca gaggtgaaat tggtaaccct ggcagagatg gtgctcgtgg tgctcctggt
1801 gctgtaggtg cccctggtcc tgctggagcc acaggtgacc ggggcgaagc tggggctgct
1861 ggtcctgctg gtcctgctgg tcctcgggga agccctggtg aacgtggtga ggtcggtcct
1921 gctggcccca atggatttgc tggtcctgct ggtgctgctg gtcaacctgg tgctaaagga
1981 gaaagaggag ccaaagggcc taagggtgaa aacggtgttg ttggtcccac aggccccgtt
2041 ggagctgctg gcccagctgg tccaaatggt ccccccggtc ctgctggaag tcgtggtgat
2101 ggaggccccc ctggtatgac tggtttccct ggtgctgctg gacggactgg tcccccagga
2161 ccctctggta tttctggccc tcctggtccc cctggtcctg ctgggaaaga agggcttcgt
2221 ggtcctcgtg gtgaccaagg tccagttggc cgaactggag aagtaggtgc agttggtccc
2281 cctggcttcg ctggtgagaa gggtccctct ggagaggctg gtactgctgg acctcctggc
2341 actccaggtc ctcagggtct tcttggtgct cctggtattc tgggtctccc tggctcgaga
2401 ggtgaacgtg gtctaccagg tgttgctggt gctgtgggtg aacctggtcc tcttggcatt
2461 gccggccctc ctggggcccg tggtcctcct ggtgctgtgg gtagtcctgg agtcaacggt
2521 gctcctggtg aagctggtcg tgatggcaac cctgggaacg atggtccccc aggtcgcgat
2581 ggtcaacccg gacacaaggg agagcgcggt taccctggca atattggtcc cgttggtgct
2641 gcaggtgcac ctggtcctca tggccccgtg ggtcctgctg gcaaacatgg aaaccgtggt
2701 gaaactggtc cttctggtcc tgttggtcct gctggtgctg ttggcccaag aggtcctagt
2761 ggcccacaag gcattcgtgg cgataaggga gagcccggtg aaaaggggcc cagaggtctt
2821 cctggcttaa agggacacaa tggattgcaa ggtctgcctg gtatcgctgg tcaccatggt
2881 gatcaaggtg ctcctggctc cgtgggtcct gctggtccta ggggccctgc tggtccttct
2941 ggccctgctg gaaaagatgg tcgcactgga catcctggta cagttggacc tgctggcatt
3001 cgaggccctc agggtcacca aggccctgct ggcccccctg gtccccctgg ccctcctgga
3061 cctccaggtg taagcggtgg tggttatgac tttggttacg atggagactt ctacagggct
3121 gaccagcctc gctcagcacc ttctctcaga cccaaggact atgaagttga tgctactctg
3181 aagtctctca acaaccagat tgagaccctt cttactcctg aaggctctag aaagaaccca
3241 gctcgcacat gccgtgactt gagactcagc cacccagagt ggagcagtgg ttactactgg
3301 attgacccta accaaggatg cactatggat gctatcaaag tatactgtga tttctctact
3361 ggcgaaacct gtatccgggc ccaacctgaa aacatcccag ccaagaactg gtataggagc
3421 tccaaggaca agaaacacgt ctggctagga gaaactatca atgctggcag ccagtttgaa
3481 tataatgtag aaggagtgac ttccaaggaa atggctaccc aacttgcctt catgcgcctg
3541 ctggccaact atgcctctca gaacatcacc taccactgca agaacagcat tgcatacatg
3601 gatgaggaga ctggcaacct gaaaaaggct gtcattctac agggctctaa tgatgttgaa
3661 cttgttgctg agggcaacag caggttcact tacactgttc ttgtagatgg ctgctctaaa
3721 aagacaaatg aatggggaaa gacaatcatt gaatacaaaa caaataagcc atcacgcctg
3781 cccttccttg atattgcacc tttggacatc ggtggtgctg accaggaatt ctttgtggac
3841 attggcccag tctgtttcaa atgtacagac tacaaagacc atgacggtga ttataaagat
3901 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4081 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
4141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
4201 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
4261 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
4321 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
4381 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
4441 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
4501 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4621 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4681 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4921 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4981 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
5041 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
5101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
5161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
5221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
5281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
7141 tcacatgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A2-FLAG质粒载体应用举例
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