| 质粒名称: | pDONR221_SLC22A31 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 132036 |
| 存储实验室: | RESOLUTE Consortium, Giulio Superti-Furga |
|
载体骨架基本信息 |
|
| 载体名称: | pDONR221 |
| 载体出品公司: | Thermo Fisher Scientific |
| 空载体大小: | 2550 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SLC22A31 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1674 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | M13-fwd |
| 3’测序引物 | M13-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDONR221_SLC22A31 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 132036 ; http://n2t.net/addgene:132036 ; RRID:Addgene_132036) |
买家导航
pDONR221SLC22A31载体基本信息
pDONR221SLC22A31载体图谱质粒图谱和多克隆位点信息
pDONR221SLC22A31载体简介
pDONR221SLC22A31载体序列
LOCUS pDONR221_SLC22A31 4224 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning
into custom expression vectors.
ACCESSION .
VERSION .
KEYWORDS pDONR221_SLC22A31
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4224)
TITLE RESOLUTE consortium SLC collection
REFERENCE 2 (bases 1 to 4224)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4224
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 92..109
/label=L4440
/note="L4440 vector, forward primer"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 522..544
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 536..553
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(2349..2448)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(2465..2484)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2466..2484)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2489..2505)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2489..2505)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2607..2626)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 2618..3427
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(2685..2704)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 3574..4162
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4063..4082
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agcaggctcc accatggagc aggaggcacg cgtgctgagg gcagcaggag gcttcggaag
721 agcacggaga ctgctggcat ccgcctcttg ggtgccatgc atcgtgctgg gcctggtgct
781 gagctccgag gagctgctga ccgcacagcc agcacctcac tgtaggccag accccacact
841 gctgccacct gccctgagag ccctgagggg acccgccctg ctggatgcag caatcccaag
901 actgggacct accagggcac cagcagaggc cctgggcgtg ctgagcccat cctacctggc
961 acctctgaca agggcaccta ggccatctag ctgggcatct tgcagcggag cagcagcagg
1021 acctacctgg aacctggtgt gcggcgacgg atggaaggtg ccactggagc aggtgagcca
1081 cctgctggga tggctgctgg gatgcgtgat cctgggagca ggatgtgata ggttcggcag
1141 gcgcgccgtg tttgtggcat ccctggtgct gaccacagga ctgggagcat ctgaggccct
1201 ggcagcaagc ttccccaccc tgctggtgct gaggctgctg cacggaggca cactggcagg
1261 cgccctgctg gccctgtatc tggccagact ggagctgtgc gacccaccac acaggctggc
1321 cttctccatg ggagcaggcc tgttttctgt ggtgggcacc ctgctgctgc ctggactggc
1381 cgccctggtg caggattgga gactgctgca gggactgggc gccctgatgt ccggcctgct
1441 gctgctgttc tggggctttc cagccctgtt cccagagtct ccctgttggc tgctggcaac
1501 aggacaggtg gcaagggcaa ggaagatcct gtggcggttt gcagaggcat ctggagtggg
1561 accaggcgac tcctctctgg aggagaacag cctggccacc gccacagagc tgaccatgct
1621 gtctgcccgc agcccacagc ctaggtacca cagccctctg ggactgctga ggaccagagt
1681 gacatggcgc aatggcctga tcctgggctt cagctccctg gtgggaggag gaatcagggc
1741 ctcctttcgg agatctctgg cccctcaggt gccaacattc tacctgcctt attttctgga
1801 ggcaggactg gaggcagccg ccctggtgtt cctgctgctg accgcagatt gctgtggcag
1861 gcgcccagtg ctgctgctgg gaaccatggt gacaggactg gcaagcctgc tgctgctggc
1921 aggagcacag tatctgccag gctggacagt gctgtttctg tccgtgctgg gactgctggc
1981 aagcagagcc gtgtccgccc tgtctagcct gttcgcagca gaggtgtttc ccacagtgat
2041 caggggagca ggactgggac tggtgctggg agccggcttc ctgggccagg ccgccggccc
2101 tctggacacc ctgcacggcc gccagggctt ctttctgcag caggtggtgt ttgcatccct
2161 ggccgtgctg gccctgctgt gcgtgctgct gctgccagag tccaggtcta ggggactgcc
2221 acagagcctg caggacgcag ataggctgcg gagatcccca ctgctgaggg gcagacccag
2281 gcaggatcac ctgcctctgc tgcctccaag caattcctac tgggccggcc acacccccga
2341 gcagcacgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
2401 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
2461 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
2521 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
2581 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
2641 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
2701 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
2761 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
2821 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
2881 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
2941 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
3001 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
3061 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
3121 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
3181 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
3241 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
3301 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
3361 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
3421 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
3481 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
3541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
3601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
3661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
3721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
3781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
3841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
3901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
3961 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
4021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
4081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
4141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
4201 ttgctggcct tttgctcaca tgtt
//
