YIplac204

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: YIplac204
质粒类型: 酿酒酵母表达载体;酵母整合型载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lacZ
载体大小: 3545 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: TRP1
表达菌株: 酿酒酵母、白色念珠菌
备注: --
产品目录号: #87591
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

YIplac204载体图谱



YIplac204 多克隆位点

载体简介

载体序列

LOCUS       YIplac204	3545 bp 	DNA	SYN
DEFINITION  YIplac204
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="YIplac204"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    634..656
                     /label="pGEX_3_primer"
     gene            complement(775..1449)
                     /label="TRP1"
                     /gene="TRP1"
     CDS             complement(775..1449)
                     /label="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        1674..1702
                     /label="AmpR_promoter"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     gene            1744..2604
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             1744..2604
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2759..3378
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
  541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
  601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
  661 caccgtcatc accgaaacgc gcgagacgaa agggcgatct tttatgcttg cttttcaaaa
  721 ggcttgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt
  781 cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac
  841 acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc
  901 tggcgtcagt ccaccagcta acataaaatg taagctctcg gggctctctt gccttccaac
  961 ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa
 1021 caagggaata aacgaatgag gtttctgtga agctgcactg agtagtatgt tgcagtcttt
 1081 tggaaatacg agtcttttaa taactggcaa accgaggaac tcttggtatt cttgccacga
 1141 ctcatctcca tgcagttgga cgatatcaat gccgtaatca ttgaccagag ccaaaacatc
 1201 ctccttaggt tgattacgaa acacgccaac caagtatttc ggagtgcctg aactattttt
 1261 atatgctttt acaagacttg aaattttcct tgcaataacc gggtcaattg ttctctttct
 1321 attgggcaca catataatac ccagcaagtc agcatcggaa tctagtgcac attctgcggc
 1381 ctctgtgctc tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat
 1441 aacagacata ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag
 1501 tcaccaatgc cctccctctt ggccctctcc ttttcttttt tcgaccgaat tggcctcgtg
 1561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
 1621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
 1681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
 1741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
 1801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
 1861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
 1921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
 1981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
 2041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
 2101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
 2161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
 2221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
 2281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
 2341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
 2401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
 2461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
 2521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
 2581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
 2641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
 2701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
 2761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
 2821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
 2881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
 2941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
 3001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
 3061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
 3121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
 3181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
 3241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
 3301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
 3361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
 3421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
 3481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
 3541 gaaga
//

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