| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-CherryPicker2 |
| 质粒类型: | 慢病毒载体；双顺反子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8394 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | CherryPicker(C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-CherryPicker2是双顺反子载体，目的基因与CherryPicker2报告基因以双顺反子的形式表达； CherryPicker是mCherry与转铁蛋白受体膜锚定结构域融合蛋白。 |
| 产品目录号: | 632581 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-CherryPicker2 8394 bp DNA circular 11-APR-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 632581,632575
FEATURES Location/Qualifiers
misc_feature 2842..3419
/label=IRES
LTR 1..635
/label=5'\LTR
misc_binding 636..653
/label=PBS
misc_feature 685..822
/label=Packaging\signal
misc_binding 1303..1536
/label=RRE
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 2803..2840
/label=MCS
misc_feature 4363..4954
/label=WPRE
LTR 5157..5793
/label=3'\LTR
rep_origin complement(6262..6935)
/label=pUC\origin
CDS complement(7080..8076)
/label=Amp(R)
CDS 3417..4346
/label=CherryPicker
misc_feature 4347..4349
/label=Stop\codon
BASE COUNT 2217 a 2036 c 2173 g 1968 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa
2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
3241 tcacctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
3361 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
3421 gaatggtgga cggcgacaac agccacgtgg agatgaagct ggccgtggac gaggaagaga
3481 acgccgacaa caacaccaag gccaacgtga ccaagcccaa gcggtgcagc ggcagcatct
3541 gctacggcac aatcgccgtg atcgtgttct tcctgatcgg cttcatgatc ggctacctgg
3601 gctactgcaa gtctagcgac ggccctggcg agacaggcat ggtgtccaag ggcgaagagg
3661 acaacatggc catcatcaaa gaattcatgc ggttcaaggt gcacatggaa ggcagcgtga
3721 acggccacga gttcgagatc gagggcgagg gcgaaggcag accttacgag ggcacccaga
3781 ccgccaagct gaaagtgacc aagggcggac ccctgccctt tgcctgggac atcctgagcc
3841 cccagttcat gtacggcagc aaggcctacg tgaagcaccc cgccgacatc cccgactacc
3901 tgaagctgag cttccccgag ggcttcaagt gggagagagt gatgaacttc gaggacggcg
3961 gcgtggtcac cgtgacccag gacagcagcc tgcaggacgg cgagttcatc tacaaagtga
4021 agctgcgggg caccaacttc cctagcgacg gccccgtgat gcagaaaaag accatgggct
4081 gggaggccag cagcgagaga atgtaccccg aggatggcgc cctgaagggc gagatcaagc
4141 agcggctgaa gctgaaggat ggcggccact acgacgccga agtgaaaacc acctacaagg
4201 ccaagaaacc cgtgcagctg cctggcgcct acaacgtgaa catcaagctg gacatcacca
4261 gccacaacga ggactacacc atcgtggagc agtacgagag agccgagggc agacacagca
4321 ccggcggcat ggacgagctg tacaaatgaa cgcgtctgga acaatcaacc tctggattac
4381 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
4441 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
4501 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
4561 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
4621 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
4681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
4741 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
4801 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
4861 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
4921 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
4981 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
5041 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
5101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
5161 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
5221 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
5281 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
5341 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
5401 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
5461 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
5521 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
5581 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
5641 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
5701 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
5761 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
5821 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
5881 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
5941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
6001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
6061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
6121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6721 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6961 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
7021 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
7081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
7621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
7681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
7741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
7981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
8041 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
8101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
8161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
8221 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
8281 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
8341 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
