pLVX-CherryPicker2

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-CherryPicker2
质粒类型: 慢病毒载体;双顺反子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8394 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: CherryPicker(C-端)
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-CherryPicker2是双顺反子载体,目的基因与CherryPicker2报告基因以双顺反子的形式表达;
CherryPicker是mCherry与转铁蛋白受体膜锚定结构域融合蛋白。
产品目录号: 632581
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-CherryPicker2载体图谱



pLVX-CherryPicker2 多克隆位点

pLVX-CherryPicker2 载体特征

载体简介

载体序列

LOCUS       pLVX-CherryPicker2   8394 bp    DNA   circular            11-APR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632581,632575
FEATURES             Location/Qualifiers
     misc_feature    2842..3419
                     /label=IRES
     LTR             1..635
                     /label=5'\LTR
     misc_binding    636..653
                     /label=PBS
     misc_feature    685..822
                     /label=Packaging\signal
     misc_binding    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    2803..2840
                     /label=MCS
     misc_feature    4363..4954
                     /label=WPRE
     LTR             5157..5793
                     /label=3'\LTR
     rep_origin      complement(6262..6935)
                     /label=pUC\origin
     CDS             complement(7080..8076)
                     /label=Amp(R)
     CDS             3417..4346
                     /label=CherryPicker
     misc_feature    4347..4349
                     /label=Stop\codon
BASE COUNT     2217 a      2036 c      2173 g      1968 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 
     2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 
     2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 
     2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 
     3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 
     3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 
     3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 
     3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 
     3241 tcacctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 
     3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg 
     3361 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg 
     3421 gaatggtgga cggcgacaac agccacgtgg agatgaagct ggccgtggac gaggaagaga 
     3481 acgccgacaa caacaccaag gccaacgtga ccaagcccaa gcggtgcagc ggcagcatct 
     3541 gctacggcac aatcgccgtg atcgtgttct tcctgatcgg cttcatgatc ggctacctgg 
     3601 gctactgcaa gtctagcgac ggccctggcg agacaggcat ggtgtccaag ggcgaagagg 
     3661 acaacatggc catcatcaaa gaattcatgc ggttcaaggt gcacatggaa ggcagcgtga 
     3721 acggccacga gttcgagatc gagggcgagg gcgaaggcag accttacgag ggcacccaga 
     3781 ccgccaagct gaaagtgacc aagggcggac ccctgccctt tgcctgggac atcctgagcc 
     3841 cccagttcat gtacggcagc aaggcctacg tgaagcaccc cgccgacatc cccgactacc 
     3901 tgaagctgag cttccccgag ggcttcaagt gggagagagt gatgaacttc gaggacggcg 
     3961 gcgtggtcac cgtgacccag gacagcagcc tgcaggacgg cgagttcatc tacaaagtga 
     4021 agctgcgggg caccaacttc cctagcgacg gccccgtgat gcagaaaaag accatgggct 
     4081 gggaggccag cagcgagaga atgtaccccg aggatggcgc cctgaagggc gagatcaagc 
     4141 agcggctgaa gctgaaggat ggcggccact acgacgccga agtgaaaacc acctacaagg 
     4201 ccaagaaacc cgtgcagctg cctggcgcct acaacgtgaa catcaagctg gacatcacca 
     4261 gccacaacga ggactacacc atcgtggagc agtacgagag agccgagggc agacacagca 
     4321 ccggcggcat ggacgagctg tacaaatgaa cgcgtctgga acaatcaacc tctggattac 
     4381 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 
     4441 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 
     4501 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 
     4561 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 
     4621 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 
     4681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     4741 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 
     4801 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 
     4861 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 
     4921 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag 
     4981 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc 
     5041 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac 
     5101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 
     5161 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 
     5221 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 
     5281 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 
     5341 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 
     5401 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 
     5461 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 
     5521 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 
     5581 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 
     5641 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     5701 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     5761 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
     5821 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 
     5881 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     5941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     6001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     6061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     6121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     6181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     6241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     6301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     6361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     6421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     6481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     6541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     6601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     6661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     6721 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     6781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6901 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     6961 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     7021 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     7081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     7141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     7321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     7381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     7441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     7501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     7621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     7681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     7741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     7861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     7981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     8041 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt 
     8101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     8161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     8221 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 
     8281 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 
     8341 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt 
//


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