pLOX-TERT-iresTK

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pLOX-TERT-iresTK
质粒类型: 慢病毒系统载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 13117bp
5' 测序引物及序列: CMV fwd: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: SP6: ATTTAGGTGACACTATAG
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: 博来霉素(Zeocin)
备注: 克隆菌株为Stbl3和HB101。
产品目录号: 12245
稳定性: --
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLOX-TERT-iresTK载体图谱



载体简介

 CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. 
During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites 
end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised. 

载体序列

LOCUS       pLOX-TERT-iresTK	13117 bp 	DNA	SYN
DEFINITION  pLOX-TERT-iresTK
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="pLOX-TERT-iresTK"
                     /mol_type="other DNA"
     misc_feature    457..637
                     /label="HIV-1_5_LTR"
     misc_feature    457..637
                     /label="truncHIV-1_3_LTR"
     misc_feature    748..792
                     /label="HIV-1_psi_pack"
     CDS             1180..2028
                     /label="ORF frame 1"
                     
     misc_feature    1302..1535
                     /label="RRE"
                     
     promoter        2040..2544
                     /label="CMV_immearly_promoter"
                    
     misc_feature    2047..2334
                     /label="CAG_enhancer"
                     
     misc_feature    2501..2521
                     /label="CMV_fwd_primer"
                     
     promoter        2502..2571
                     /label="CMV_promoter"
                     
     misc_feature    2545..2564
                     /label="pCEP_fwd_primer"
                     
     misc_feature    2547..2571
                     /label="LNCX_primer"
                    
     CDS             complement(2622..3305)
                     /label="ORF frame 3"
                     
     CDS             2691..6089
                     /label="ORF frame 3"
                    
     CDS             complement(3726..4946)
                     /label="ORF frame 3"
                    
     misc_feature    6118..6608
                     /label="IRES"
                    
     CDS             6612..7565
                     /label="ORF frame 3"
                    
     misc_feature    7609..7630
                     /label="U3PPT"
                     
     misc_feature    7609..7624
                     /label="cPPT"
                     
     misc_feature    7681..7714
                     /label="loxP"
                   
     misc_feature    7734..7914
                     /label="HIV-1_5_LTR"
                    
     misc_feature    7734..7914
                     /label="truncHIV-1_3_LTR"
                     
     misc_feature    complement(7940..7957)
                     /label="Sp6_primer"
                   
     gene            8195..8458
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     
     misc_feature    complement(8247..8266)
                     /label="pBRrevBam_primer"
                     
     misc_feature    8599..8621
                     /label="pGEX_3_primer"
                     
     promoter        8780..8808
                     /label="AmpR_promoter"
                    
     gene            8850..9710
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     
     CDS             8850..9710
                     /label="ORF frame 3"
                     
     rep_origin      9865..10484
                     /label="pBR322_origin"
                    
     misc_feature    complement(10710..10730)
                     /label="pBABE_3_primer"
                   
     misc_feature    complement(10716..11169)
                     /label="SV40_enhancer"
                     
     promoter        10728..10996
                     /label="SV40_promoter"
                     
     rep_origin      10895..10972
                     /label="SV40_origin"
                     
     misc_feature    10957..10976
                     /label="SV40pro_F_primer"
                    
     misc_feature    12177..12192
                     /label="SV40_int"
                     
     misc_feature    12198..12245
                     /label="SV40_3_splice"
                    
     CDS             12322..12795
                     /label="ORF frame 1"
                     
     misc_feature    12373..12393
                     /label="SV40 NLS"
                     
     misc_feature    12379..12393
                     /label="NLS"
                    
     terminator      12821..12952
                     /label="SV40_PA_terminator"
                   
     misc_feature    12909..12928
                     /label="EBV_rev_primer"
                   
ORIGIN
    1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
   61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
  121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
  181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
  241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
  301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
  361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
  421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
  481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
  541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
  601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
  661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
  721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
  781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
  841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
  901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
  961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc
 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctacta
 2641 gtgaattcct gctgcgcacg tgggaagccc tggccccggc cacccccgcg atgccgcgcg
 2701 ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc
 2761 tggccacgtt cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc
 2821 cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc
 2881 cgccccccgc cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag
 2941 tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc
 3001 tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc
 3061 ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ttgcgccgcg
 3121 tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc
 3181 ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg
 3241 cccggccccc gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga
 3301 accatagcgt cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc
 3361 gcgggggcag tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc
 3421 ctgagccgga gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg
 3481 gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct
 3541 ctttggaggg tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc
 3601 acgcgggccc cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg
 3661 tgtacgccga gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct
 3721 ccttcctact cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca
 3781 tctttctggg ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc
 3841 agcgctactg gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc
 3901 cctacggggt gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg
 3961 gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag
 4021 acccccgtcg cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct
 4081 tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg
 4141 aacgccgctt cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct
 4201 cgctgcagga gctgacgtgg aagatgagcg tgcggggctg cgcttggctg cgcaggagcc
 4261 caggggttgg ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt
 4321 tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca
 4381 cggagaccac gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt
 4441 tgcaaagcat tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag
 4501 cagaggtcag gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca
 4561 tccccaagcc tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa
 4621 cgttccgcag agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg
 4681 tgctcaacta cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg
 4741 acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc
 4801 ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca
 4861 ggctcacgga ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt
 4921 atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct
 4981 ctaccttgac agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca
 5041 gcccgctgag ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg
 5101 gcctcttcga cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt
 5161 cctacgtcca gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc
 5221 tgtgctacgg cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc
 5281 tgcgtttggt ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc
 5341 tcaggaccct ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag
 5401 tggtgaactt ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg
 5461 cccacggcct attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga
 5521 gcgactactc cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct
 5581 tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca
 5641 gcctgtttct ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga
 5701 tcctcctgct gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc
 5761 aagtttggaa gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct
 5821 actccatcct gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc
 5881 ctctgccctc cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc
 5941 gacaccgtgt cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga
 6001 gtcggaagct cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc
 6061 cctcagactt caagaccatc ctggactgat ggcgtcgaca ttaattccgg ttattttcca
 6121 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
 6181 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
 6241 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
 6301 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
 6361 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
 6421 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
 6481 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
 6541 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
 6601 atgataatac catggcttcg tacccctgcc atcaacacgc gtctgcgttc gaccaggctg
 6661 cgcgttctcg cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag
 6721 ccacggaagt ccgcctggag cagaaaatgc ccacgctact gcgggtttat atagacggtc
 6781 ctcacgggat ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg
 6841 atatcgtcta cgtacccgag ccgatgactt actggcaggt gctgggggct tccgagacaa
 6901 tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg
 6961 cggcggtggt aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg
 7021 ccgttctggc tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc
 7081 tcaccctcat cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcgat
 7141 accttatggg cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga
 7201 ccttgcccgg cacaaacatc gtgttggggg cccttccgga ggacagacac atcgaccgcc
 7261 tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc tatgctggcc gcgattcgcc
 7321 gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca gggcggcggg tcgtggcggg
 7381 aggattgggg acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca
 7441 acgcgggccc acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt
 7501 tgctggcccc caacggcgac ctgtataacg tgtttgcctg ggctttggct cgacggtacc
 7561 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
 7621 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga
 7681 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc
 7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
 7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
 7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
 7921 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
 7981 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
 8041 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
 8101 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
 8161 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
 8221 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
 8281 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
 8341 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
 8401 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
 8461 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
 8521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
 8581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
 8641 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
 8701 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
 8761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
 8821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
 8881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
 8941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
 9001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
 9061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
 9121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
 9181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
 9241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
 9301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
 9361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
 9421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
 9481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
 9541 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
 9601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
 9661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
 9721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
 9781 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
 9841 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
 9901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
 9961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
10021 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
10081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
10141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
10201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
10261 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
10321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
10381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
10441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
10501 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
10561 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
10621 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
10681 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
10741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
10801 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
10861 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
10921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
10981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
11041 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
11101 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
11161 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
11221 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
11281 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
11341 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
11401 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
11461 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
11521 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
11581 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
11641 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
11701 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
11761 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
11821 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
11881 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
11941 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
12001 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
12061 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
12121 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
12181 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
12241 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
12301 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
12361 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
12421 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
12481 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
12541 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
12601 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
12661 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
12721 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
12781 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
12841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
12901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
12961 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
13021 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
13081 gaaattgtat ttgttaaata tgtactacaa acttagt
//

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