pKA/cAMP-mGreenLantern-iPAK4

LOCUS       pKA/cAMP-mGreenLantern-iPAK4            5152 bp DNA     circular SYN 19-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Rui
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1167..2243
                     /mol_type="genomic DNA"
                     /organism="."
     polyA_signal    9..57
                     /note="synthetic polyadenylation signal"
     misc_feature    71..162
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    196..282
                     /label=CRE regulatory seq
     promoter        289..435
                     /label=P_TAL
     primer_bind     422..465
                     /label=cAMP-mGL-F
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             450..1166
                     /codon_start=1
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(1151..1192)
                     /label=INKA-mGreenLantern-R
     CDS             1179..1292
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             1299..2240
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(1392..1410)
                     /label=PAK4-R
     primer_bind     complement(2223..2266)
                     /label=XbaI-PAK4-R
     polyA_signal    2284..2405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2824..3412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3583..4443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4444..4548)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4575..5030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
       61 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
      121 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct
      181 cttacgcgtg ctagcgcacc agacagtgac gtcagctgcc agatcccatg gccgtcatac
      241 tgtgacgtct ttcagacacc ccattgacgt caatgggaga acagatctgc cgccccgact
      301 gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt gtcatcatag
      361 aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc gagcaacggg
      421 ccacggggat gaagcagaag cttgccacca tggtgagcaa gggcgaggag ctgttcaccg
      481 gcgtggtgcc tatcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga
      541 ggggcgaggg cgagggcgac gccaccaacg gcaagctgac cctgaagttc atctgcacca
      601 ccggcaagct gcctgtgcct tggcctaccc tggtgaccac cctgggctac ggcgtggcct
      661 gcttcgccag gtaccctgac cacatgaagc agcacgactt cttcaagagc gccatgcctg
      721 agggctacgt gcaggagagg accatcagct tcaaggacga cggcacctac aagaccaggg
      781 ccgaggtgaa gttcgagggc gacaccctgg tgaacaggat cgtgctgaag ggcatcgact
      841 tcaaggagga cggcaacatc ctgggccaca agctggagta caacttcaac agccacaagg
      901 tgtacatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag accaggcaca
      961 acgtggagga cggcggcgtg cagctggccg accactacca gcagaacacc cctatcggcg
     1021 acggccctgt gctgctgcct gacaaccact acctgagcca ccagagcaag ctgagcaagg
     1081 accctaacga gaagagggac cacatggtgc tgaaggagag ggtgaccgcc gccggcatca
     1141 cccacgacat ggacgagctg tacaagtcag gtggatccga agcagaggac tggacagcag
     1201 ccctactgaa caggggtcgc agtcgccagc ccctggtact aggggacaat tgctttgctg
     1261 acttggtgca caactggatg gagctgcctg aggaattccc cgccgcccct gctgttcctg
     1321 ggccccctgg cccccgctca ccacagcggg agccacagcg agtatcccat gagcagttcc
     1381 gggctgccct gcagctggtg gtggacccag gcgacccccg ctcctacctg gacaacttca
     1441 tcaagattgg cgagggctcc acgggcatcg tgtgcatcgc caccgtgcgc agctcgggca
     1501 agctggtggc cgtcaagaag atggacctgc gcaagcagca gaggcgcgag ctgctcttca
     1561 acgaggtggt aatcatgagg gactaccagc acgagaatgt ggtggagatg tacaacagct
     1621 acctggtggg ggacgagctc tgggtggtca tggagttcct ggaaggaggc gccctcaccg
     1681 acatcgtcac ccacaccagg atgaacgagg agcagatcgc agccgtgtgc cttgcagtgc
     1741 tgcaggccct gtcggtgctc cacgcccagg gcgtcatcca ccgggacatc aagagcgact
     1801 cgatcctgct gacccatgat ggcagggtga agctgtcaga ctttgggttc tgcgcccagg
     1861 tgagcaagga agtgccccga aggaagtcgc tggtcggcac gccctactgg atggccccag
     1921 agctcatctc ccgccttccc tacgggccag aggtagacat ctggtcgctg gggataatgg
     1981 tgattgagat ggtggacgga gagcccccct acttcaacga gccacccctc aaagccatga
     2041 agatgattcg ggacaacctg ccaccccgac tgaagaacct gcacaaggtg tcgccatccc
     2101 tgaagggctt cctggaccgc ctgctggtgc gagaccctgc ccagcgggcc acggcagccg
     2161 agctgctgaa gcacccattc ctggccaagg cagggccgcc tgccagcatc gtgcccctca
     2221 tgcgccagaa ccgcaccaga tgaatctaga gtcggggcgg ccggccgctt cgagcagaca
     2281 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2341 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2401 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2461 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2521 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2581 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     2641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3241 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4561 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4621 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4921 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4981 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc
     5041 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5101 gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg ga
//