pC-GoldyTALEN

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pC-GoldyTALEN 
出品公司: 爱迪基因
目录编号: 38143
存储实验室: Daniel Carlson

载体骨架基本信息
载体名称: pBSK
载体抗性: 氨苄霉素
空载体大小: 4000 bp
完整质粒大小: 5702 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: GoldyTALEN
物种来源: 合成
插入大小: 1800 bp
基因突变: Truncate at N and C terminous
启动子 miniCaggs
标签/融合蛋白: FokI homodimer(C端)    AcV5(N端)
克隆方法: 限制性内切酶
5' 克隆位点: Esp31(在克隆期间被破坏)
3' 克隆位点: Esp31(在克隆期间被破坏)
5' 测序引物 CGTGACCGCAATGGAGGC
3' 测序引物 CACTGCATCCATGGCAGG

文献引用方法
材料和方法部分: pC-GoldyTALEN was a gift from Daniel Carlson (Addgene plasmid # 38143)
参考文献部分: Efficient TALEN-mediated gene knockout in livestock. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC. Proc Natl Acad Sci U S A. 2012 Oct 1. 10.1073/pnas.1211446109 PubMed 23027955

载体图谱质粒图谱和多克隆位点信息

pC-GoldyTALEN载体图谱

载体简介

载体序列

LOCUS       pC-GoldyTALEN	5701 bp 	DNA	SYN
DEFINITION  pC-GoldyTALEN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="pC-GoldyTALEN"
                     /mol_type="other DNA"
     misc_feature    108..395
                     /label="CAG_enhancer"
     misc_feature    940..959
                     /label="pCAG_F_primer"
     CDS             1047..1688
                     /label="ORF frame 3"
     misc_feature    1062..1082
                     /label="SV40 NLS"
     misc_feature    1068..1082
                     /label="NLS"
     misc_feature    complement(1544..1699)
                     /label="lacZ_a"
     misc_feature    1670..1692
                     /label="M13_pUC_fwd_primer"
     promoter        1685..1701
                     /label="M13_forward20_primer"
     promoter        1711..1729
                     /label="T7_promoter"
     promoter        complement(1776..1795)
                     /label="T3_promoter"
     promoter        complement(1812..1830)
                     /label="M13_reverse_primer"
     misc_feature    complement(1829..1851)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1865..1894)
                     /label="lac_promoter"
     CDS             1909..2709
                     /label="ORF frame 1"
     misc_feature    complement(2788..2807)
                     /label="EBV_rev_primer"
     misc_feature    complement(2993..3012)
                     /label="EBV_rev_primer"
     rep_origin      complement(3403..3709)
                     /label="f1_origin"
     promoter        3788..3816
                     /label="AmpR_promoter"
     gene            3858..4718
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             3858..4718
                     /label="ORF frame 3"
     rep_origin      4873..5492
                     /label="pBR322_origin"
ORIGIN
    1 tcattaatgc agctgagatc ttaagggatt actagtttaa acctagttat taatagtaat
   61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
  121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
  181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
  241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
  301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
  361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
  421 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
  481 ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc
  541 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
  601 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
  661 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
  721 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
  781 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
  841 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagctaga gcctctgcta
  901 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
  961 tgtctcatca ttttggcaaa gaattttaag ctaggatcta actcgagaaa gatattgtat
 1021 atatcgtaac aataggaggt tcaacaatgg cttcctcccc tccaaagaaa aagagaaagg
 1081 ttagttggaa ggacgcaagt ggttggtcta gagtggatct acgcacgctc ggctacagtc
 1141 agcagcagca agagaagatc aaaccgaagg tgcgttcgac agtggcgcag caccacgagg
 1201 cactggtggg ccatgggttt acacacgcgc acatcgttgc gctcagccaa cacccggcag
 1261 cgttagggac cgtcgctgtc acgtatcagc acataatcac ggcgttgcca gaggcgacac
 1321 acgaagacat cgttggcgtc ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc
 1381 tcacggatgc gggggagttg agaggtccgc cgttacagtt ggacacaggc caacttgtga
 1441 agattgcaaa acgtggcggc gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac
 1501 tgacgggtgc ccccctggag acgggcgccg ctacagggcg cgtcccattc gccattcagg
 1561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
 1621 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
 1681 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
 1741 accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg
 1801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
 1861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
 1921 actcacatta attgcgttgc gctcactgcc cgctttccac cggtcgtctc caacgaccac
 1981 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
 2041 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
 2101 cgcgttgcct ctagatccca gctagtgaaa tctgaattgg aagagaagaa atctgaactt
 2161 agacataaat tgaaatatgt gccacatgaa tatattgaat tgattgaaat cgcaagaaat
 2221 tcaactcagg atagaatcct tgaaatgaag gtgatggagt tctttatgaa ggtttatggt
 2281 tatcgtggta aacatttggg tggatcaagg aaaccagacg gagcaattta tactgtcgga
 2341 tctcctattg attacggtgt gatcgttgat actaaggcat attcaggagg ttataatctt
 2401 ccaattggtc aagcagatga aatgcaaaga tatgtcgaag agaatcaaac aagaaacaag
 2461 catatcaacc ctaatgaatg gtggaaagtc tatccatctt cagtaacaga atttaagttc
 2521 ttgtttgtga gtggtcattt caaaggaaac tacaaagctc agcttacaag attgaatcat
 2581 atcactaatt gtaatggagc tgttcttagt gtagaagagc ttttgattgg tggagaaatg
 2641 attaaagctg gtacattgac acttgaggaa gtgagaagga aatttaataa cggtgagata
 2701 aacttttaat aggagctcgc ccggggatct aattcaatta gagactaatt caattagagc
 2761 taattcaatt aggatccaga attcattgat gagtttggac aaaccacaac tagaatgcag
 2821 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
 2881 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
 2941 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca ttgatgagtt
 3001 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
 3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
 3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
 3181 ctacaaatgt ggtcattcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
 3241 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
 3301 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
 3361 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
 3421 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
 3481 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
 3541 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
 3601 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
 3661 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
 3721 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
 3781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
 3841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
 3901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
 3961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
 4021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
 4081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
 4141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
 4201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
 4261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
 4321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
 4381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
 4441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
 4501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
 4561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
 4621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
 4681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
 4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
 4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
 4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
 4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
 4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
 5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
 5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
 5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
 5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
 5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
 5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
 5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
 5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
 5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
 5581 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
 5641 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
 5701 t
//

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