| 质粒名称: | 4xCSL-luciferase |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 41726 |
| 存储实验室: | Raphael Kopan |
|
载体骨架基本信息 |
|
| 载体名称: | RSV-TATA pGL2 |
| 载体出品公司: | Dr. D. Towler (Washington University, St. Louis) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 从pGL2碱性：劳斯肉瘤病毒TATA盒插入降低基础荧光素酶活性。 |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 4xCSL binding sites,4xCBS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | Contains a multimerized high affinity CSL binding site (4 x CGTGGGAA) |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 萤火虫荧光素酶（主链C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII/BamHI（在克隆过程中销毁） |
| 3' 克隆位点: | BglII/BamHI（在克隆过程中销毁） |
| 5' 测序引物 | F1ori-F (GTGGACTCTTGTTCCAAACTGG) |
| 3' 测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | 4xCSL-luciferase was a gift from Raphael Kopan (Addgene plasmid # 41726 ; http://n2t.net/addgene:41726 ; RRID:Addgene_41726) |
| 参考文献部分: | Murine notch homologs (N1-4) undergo presenilin-dependent proteolysis. Saxena MT, Schroeter EH, Mumm JS, Kopan R. J Biol Chem. 2001 Oct 26;276(43):40268-73. Epub 2001 Aug 22. 10.1074/jbc.M107234200 PubMed 11518718 |
买家导航
4xCSLluciferase载体基本信息
4xCSLluciferase载体图谱质粒图谱和多克隆位点信息
4xCSLluciferase载体简介
4xCSLluciferase载体序列
LOCUS 4xCSL-luciferase 5715 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 4xCSL-luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5715)
AUTHORS Saxena MT, Schroeter EH, Mumm JS, Kopan R
TITLE Murine notch homologs (N1-4) undergo presenilin-dependent
proteolysis.
JOURNAL J Biol Chem. 2001 Oct 26;276(43):40268-73. Epub 2001 Aug 22.
PUBMED 11518718
REFERENCE 2 (bases 1 to 5715)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5715
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 86..207
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS 425..2077
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(491..509)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
intron 2318..2383
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2513..2533
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2958..3092
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3234..3251)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3405..3993)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3485..3504)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4164..5024)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4787..4806
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5025..5129)
/gene="bla"
/label=AmpR promoter
rep_origin 5156..5611
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5243..5262)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5453..5474
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gtacgtggag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
61 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
181 tttgtccaaa ctcatcaatg tatcttatgg tactgtaact gagctaacat aacccgggga
241 attcgccgtg ggaaaatcgc cgtgggaaaa tcgccgtggg aaaatcgccg tgggaaaatt
301 cgaggatctc cgcattgcag agataattgt atttaagtgc ctagctcgat acaataaacg
361 ccatttgacc attcaccaca ttggtgtgca cctccaagct tggcattccg gtactgttgg
421 taaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga
481 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
541 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
601 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
661 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
721 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
781 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
841 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
901 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
961 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc
1021 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
1081 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
1141 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
1201 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
1261 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
1321 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
1381 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
1441 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
1501 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
1561 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
1621 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
1681 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
1741 gtctttaatt aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt
1801 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
1861 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
1921 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
1981 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
2041 cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga
2101 cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta atttttttaa
2161 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
2221 atgaatggca gaaattcgcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
2281 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
2341 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
2401 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
2461 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
2521 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
2581 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
2641 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
2701 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
2761 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
2821 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
2881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
2941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
3001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
3061 aactcatcaa tgtatcttat catgtctgga tccgtcgacc gatgcccttg agagccttca
3121 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
3181 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
3241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
3301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
3361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
3421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
3481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
3541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
4021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
4081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
4141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
4201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
4261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
4321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
4381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
4441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
4501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
4561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
4621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
4681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
4801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
5041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
5101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
5161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
5221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
5281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
5341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
5401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
5461 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
5521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
5581 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
5641 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
5701 gtaagtaata ttaag
//
