| 质粒名称: | HOP-flash |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 83467 |
| 存储实验室: | Barry Gumbiner |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4797 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 荧光素酶表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter,TEAD reporter |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 223 bp |
| 基因突变: | -- |
| GenBank编号 | 7003, 8463,7005, 7004 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | LucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | HOP-flash was a gift from Barry Gumbiner (Addgene plasmid # 83467 ; http://n2t.net/addgene:83467 ; RRID:Addgene_83467) |
| 参考文献部分: | Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway. Kim NG, Gumbiner BM. J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27. 10.1083/jcb.201501025 PubMed 26216901 |
买家导航
HOPflash载体基本信息
HOPflash载体图谱质粒图谱和多克隆位点信息
HOPflash载体简介
HOPflash载体序列
LOCUS HOP-flash 5020 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with
minimal promoter plus luciferase reporter gene.
ACCESSION .
VERSION .
KEYWORDS HOP-flash
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5020)
AUTHORS Kim NG, Gumbiner BM
TITLE Adhesion to fibronectin regulates Hippo signaling via the
FAK-Src-PI3K pathway.
JOURNAL J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025.
Epub 2015 Jul 27.
PUBMED 26216901
REFERENCE 2 (bases 1 to 5020)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5020
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 284..293
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 290..1942
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(356..374)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1983..2104
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1994..2013)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2048..2067
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2352..2369)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2523..3111)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2603..2622)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3282..4142)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3905..3924
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4143..4247)
/gene="bla"
/label=AmpR promoter
rep_origin 4274..4729
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4361..4380)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4571..4592
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4860..4908
/note="synthetic polyadenylation signal"
misc_feature 4922..5013
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4962..4981
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg cccttggtgg
61 ggaggaatgc gaggaatgtc cctgtttgtg gtggggagga atgcgaggaa tgtccctgtt
121 tgtggtgggg aggaatgcga ggaatgtccc tgtttgtggt ggggaggaat gcgaggaatg
181 tccctgttta agggcgaatt ctgcagatta gagggtatat aatggatccg gtactcgaga
241 tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc
301 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
361 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
421 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
481 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
541 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
601 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
661 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
721 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
781 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
841 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
901 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
961 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
1021 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
1081 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
1141 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
1201 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
1261 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
1321 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
1381 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
1441 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
1501 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
1561 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
1621 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
1681 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
1741 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
1801 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
1861 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
1921 gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat
1981 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
2041 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
2101 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
2161 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
2221 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
2281 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
2341 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
2401 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
2461 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
2521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
2581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
2641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
2701 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
2761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
2821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
2881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
2941 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
3001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
3061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
3121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
3181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
3241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
3301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
3361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
3421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
3481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
3541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
3781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
3841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
3901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
3961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
4201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
4261 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
4381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
4501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4561 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
4621 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
4681 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca
4741 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
4801 cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca
4861 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
4921 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
4981 ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
//
