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pmCherry-C1-hLC3载体质粒基本信息
pmCherry-C1-hLC3质粒图谱载体图谱和pmCherry-C1-hLC3载体序列质粒序列多克隆位点信息
pmCherry-C1-hLC3质粒载体简介
pmCherry-C1-hLC3质粒序列载体序列
LOCUS pmCherry-C1-hLC3 5076 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5076)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5076
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 607..616
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1320
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
CDS 1342..1716
/codon_start=1
/gene="MAP1LC3B"
/product="human LC3 protein"
/label=LC3B
/note="Atg8 family member found on autophagosomes"
/translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
EDGFLYMVYASQETFGMKLSV"
polyA_signal 1864..1985
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1992..2447)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2474..2578
/gene="bla"
/label=AmpR promoter
promoter 2580..2937
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2788..2923
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2972..3766
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3998..4045
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4374..4962
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
1321 tccggactca gatctcgagc tatgccgtcg gagaagacct tcaagcagcg ccgcaccttc
1381 gaacaaagag tagaagatgt ccgacttatt cgagagcagc atccaaccaa aatcccggtg
1441 ataatagaac gatacaaggg tgagaagcag cttcctgttc tggataaaac aaagttcctt
1501 gtacctgacc atgtcaacat gagtgagctc atcaagataa ttagaaggcg cttacagctc
1561 aatgctaatc aggccttctt cctgttggtg aacggacaca gcatggtcag cgtctccaca
1621 ccaatctcag aggtgtatga gagtgagaaa gatgaagatg gattcctgta catggtctat
1681 gcctcccagg agacgttcgg gatgaaattg tcagtgtaag gtaccgcggg cccgggatcc
1741 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
1801 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
1861 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1981 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
2041 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2101 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2161 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2221 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2281 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2341 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2401 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
2461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
2521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
2581 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
2641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
2701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
2941 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
3001 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
3061 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
3121 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
3181 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
3241 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
3301 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
3361 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
3421 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
3481 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
3541 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
3601 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
3661 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
3721 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
3781 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
3841 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
3901 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
3961 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
4021 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
4081 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
4141 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
4201 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
4261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
4321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
4381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
4441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
4501 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
4561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
4621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
4681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
4741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
4801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
4861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
4921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
4981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
5041 cctgattctg tggataaccg tattaccgcc atgcat
//
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