| 质粒名称: | pSELECT-HA-mFOXO1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 83308 |
| 存储实验室: | Steven Abcouwer |
|
载体骨架基本信息 |
|
| 载体名称: | pSELECT-puro |
| 载体出品公司: | Invivogen |
| 空载体大小: | 3390 bp |
| 完整质粒大小: | 5432 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 嘌呤霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | FoxO1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1983 bp |
| GenBank编号 | NM_ 019739.3 |
| 启动子: | hEF1/HTLV prom |
| 标签/融合蛋白: | HA（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SalI (未破坏) |
| 3’克隆位点: | NheI (未破坏) |
| 5’测序引物 | GACCCTGCTTGCTCAACTCT |
| 3’测序引物 | GTCTACGCTGCCCAGTCTGT |
|
文献引用方法 |
|
| 材料和方法部分: | pSELECT-HA-mFOXO1 was a gift from Steven Abcouwer (Addgene plasmid # 83308 ; http://n2t.net/addgene:83308 ; RRID:Addgene_83308) |
| 参考文献部分: | Insulin-like growth factor 1 rescues R28 retinal neurons from apoptotic death through ERK-mediated BimEL phosphorylation independent of Akt. Kong D, Gong L, Arnold E, Shanmugam S, Fort PE, Gardner TW, Abcouwer SF. Exp Eye Res. 2016 Aug 7;151:82-95. doi: 10.1016/j.exer.2016.08.002. 10.1016/j.exer.2016.08.002 PubMed 27511131 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSELECT-HA-mFOXO1 5432 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection
marker.
ACCESSION .
VERSION .
KEYWORDS pSELECT-HA-mFOXO1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5432)
AUTHORS Kong D, Gong L, Arnold E, Shanmugam S, Fort PE, Gardner TW, Abcouwer
SF
TITLE Insulin-like growth factor 1 rescues R28 retinal neurons from
apoptotic death through ERK-mediated BimEL phosphorylation
independent of Akt.
JOURNAL Exp Eye Res. 2016 Aug 7;151:82-95. doi: 10.1016/j.exer.2016.08.002.
PUBMED 27511131
REFERENCE 2 (bases 1 to 5432)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5432
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 9..57
/note="synthetic polyadenylation signal"
misc_feature 68..159
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 108..127
/label=RVprimer3
/note="pGL3 vector, forward primer"
promoter 196..407
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 420..688
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 739..765
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
polyA_signal 2817..2938
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2828..2847)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2882..2901
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
polyA_signal 3038..3432
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
promoter complement(4055..4073)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(4065..4111)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(4158..4209)
/label=CMVd2 promoter
/note="truncated human cytomegalovirus (CMV) immediate
early promoter that yields moderately reduced expression
relative to the full-length promoter"
primer_bind complement(4187..4207)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 4362..4665
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(4745..5333)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4825..4844)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
61 cgtaactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
121 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgaaggatc tgcgatcgct
181 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
241 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
301 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
361 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
421 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
481 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
541 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
601 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
661 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacc tgagatcacc
721 ggcgtgtcga ccgccatgta cccatacgat gttccggatt acgctgccga agcgccccag
781 gtggtggaga ccgacccgga cttcgagccg ctgccccggc agcgctcctg tacctggccg
841 ctgcccaggc cggagtttaa ccagtccaac tcgaccacct ccagtccggc gccgtcgggc
901 ggcgcggccg ccaaccccga cgccgcggcg agcctggcct cggcgtccgc tgtcagcacc
961 gactttatga gcaacctgag cctgctggag gagagtgagg acttcgcgcg ggcgccaggc
1021 tgcgtggccg tggcggcggc ggctgcggcc agcaggggcc tgtgcgggga cttccagggc
1081 cccgaggcgg gctgcgtgca cccagcgccg ccacagcccc caccgaccgg gccgctgtcg
1141 cagcccccac ccgtgcctcc ctccgctgcc gccgccgcgg ggccactcgc gggacagccg
1201 cgcaagacca gctcgtcgcg ccgcaacgcg tggggcaacc tgtcgtacgc cgacctcatc
1261 accaaggcca tcgagagctc agccgagaag aggctcaccc tgtcgcagat ctacgagtgg
1321 atggtgaaga gcgtgcccta cttcaaggat aagggcgaca gcaacagctc ggcgggctgg
1381 aagaattcaa ttcgccacaa tctgtccctt cacagcaagt ttattcgagt gcagaatgaa
1441 ggaactggaa agagttcttg gtggatgctc aatccagagg gaggcaagag cggaaaatca
1501 ccccggagaa gagctgcgtc catggacaac aacagtaaat ttgctaagag ccgagggcgg
1561 gctgctaaga aaaaagcatc tctccagtct gggcaagagg gtcctggaga cagccctggg
1621 tctcagtttt ctaagtggcc tgcgagtcct gggtcccaca gcaacgatga ctttgataac
1681 tggagtacat ttcgtcctcg aaccagctca aatgctagta ccatcagtgg gagactttct
1741 cccatcatga cagagcagga tgacctggga gatggggacg tgcattccct ggtgtatcca
1801 ccctctgctg ccaagatggc gtctacgctg cccagtctgt ctgaaatcag caatccagaa
1861 aacatggaga accttctgga taatctcaac cttctctcgt ccccaacatc tttaactgtg
1921 tccacccagt cctcgcctgg cagcatgatg cagcagacac catgctattc gtttgcaccg
1981 ccaaacacca gtctaaattc acccagtcca aactactcaa agtacacata cggccaatcc
2041 agcatgagcc ctttgcccca gatgcctatg cagacacttc aggacagcaa atcaagttac
2101 ggaggattga accagtataa ctgtgcccca ggactcttga aagagttgtt gacttctgac
2161 tctcctcccc acaatgacat tatgtcaccg gttgatcccg gagtggccca acccaacagt
2221 cgggtcctgg gccaaaatgt aatgatgggc cctaattcgg tcatgccagc gtatggcagc
2281 caggcatctc ataacaaaat gatgaacccc agctcccaca cccaccctgg acatgcacag
2341 caaacggctt cggtcaacgg ccgtaccctg ccccatgtgg tgaacaccat gcctcacaca
2401 tctgccatga accgcttgac ccccgtgaag acacctttac aagtgcctct gtcccacccc
2461 atgcagatga gtgccctggg cagctactcc tcggtgagca gctgcaatgg ctatggtagg
2521 atgggtgtcc tccaccagga gaagctccca agtgacttgg atggcatgtt tattgagcgc
2581 ttggactgtg acatggagtc catcattcgg aatgacctca tggatggaga taccttggat
2641 tttaactttg ataatgtgtt gcccaaccaa agcttcccac acagtgtcaa gactacaaca
2701 cacagctggg tgtcaggcta agagttagtg agcaggctac atttaaaagt ccttcagatt
2761 gtctgacagc aggaactgag gagcagtcca aagatgcgct agctggccag acatgataag
2821 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
2881 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2941 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
3001 aagcaagtaa aacctctaca aatgtggtat ggaattctaa aatacagcat agcaaaactt
3061 taacctccaa atcaagcctc tacttgaatc cttttctgag ggatgaataa ggcataggca
3121 tcaggggctg ttgccaatgt gcattagctg tttgcagcct caccttcttt catggagttt
3181 aagatatagt gtattttccc aaggtttgaa ctagctcttc atttctttat gttttaaatg
3241 cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa atacatcatt
3301 gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc cttcataata
3361 tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa attggacagc
3421 aagaaagcga gcttctagct caggtttaag ctccaggctt ccttgtcatg caccaagttc
3481 ttgggccttc tggaacctca acatcagctg tcacagtgaa tcccagtctt tcataaaaag
3541 gcaggtttct gggagcagaa gtttccagaa aggcaggaac tccagccctt tcagcagctt
3601 caactccagg cagaacaaca gcagatccca gaccctttcc ctggtggtca gggctcactc
3661 caacagttgc cagaaaccaa gctggctctt ttggcctgtg tggtgccagc agaccttcca
3721 tttgttgttg tgctgccagc ctgcttccag agagctcagc cattcttggt ccaatttcag
3781 caaaaacagc accagcttca acagactcag gtgttgtcca aactgcaaca gcagctccat
3841 catctgcaac ccaaactttt ccaatgtcca gtcccactct ggtgaggaag agttcttgca
3901 gttctgtcac cctctcaatg tgcctgtcag ggtcaactgt gtgccttgtt gcagggtagt
3961 ctgcaaaagc agcagccagt gttctcacag ctcttggaac atcatctctg gttgccagcc
4021 tcactgtggg tttgtactca gtcatggtgg ccctcctata gtgagtcgta ttatactatg
4081 ccgatatact atgccgatga ttaattgtca aaacagcgtg gatggcgtct ccagcttatc
4141 tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc
4201 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg atttactagt
4261 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
4321 cgctatccac gcccattgat gtactgccaa aaccgcatca tcatggtaat agcgatgact
4381 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
4441 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
4501 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
4561 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
4621 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcctgc aggttaatta
4681 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4741 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4861 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4921 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4981 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
5041 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
5101 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
5161 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
5221 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
5281 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
5341 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
5401 tggtcatggc tagttaatta acatttaaat ca
//
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