pAAVLINK-EF1a-HA-5'-BAZ2B

LOCUS       pAAVLINK-EF1a-HA-5'-BAZ2B               6852 bp DNA     circular SYN 19-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..3992
                     /codon_start=1
                     /label=BAZ2B
                     /translation="MESGERLPSSAASSTTPTSSSTPSVASVVSKGGLSTGVASLSSTI
                     NPCGHLFRTAGDQPFNLSTVSSAFPMVSHPVFGLHSASSGHSEFGGLGTLGTPTALAAH
                     PQLASFPGAEWWRTTDAHTRTGATFFPPLLGIPPLFAPPAQNHDSSSFHSRTSGKSNRN
                     GPEKGVNGSINGSNTSSVIGINTSVLSTTASSSMGQTKSTSSGGGNRKCNQEQSKNQPL
                     DARVDKIKDKKPRKKAMESSSNSDSDSCTSSDTSSEGISSSDSDDLEEDEEEEDQSIEE
                     SEDDDSDSESEAQHKSNNQVLLHGISDPKADGQKATEKAQEKRIHQPLPLASESQTHSF
                     QSQQKQPQVLSQQLPFIFQSSQAKEESVNKHTSVIQSTGLVSNVKPLSLVNQAKKETYM
                     KLIVPSPDVLKAGNKNTSEESSSLTSELRSKREQYKQAFPSQLKKQESSKSLKKVIAAL
                     SNPKATSSSPAHPKQTLENNHPNPFLTNALLGNHQPNGVIQSVIQEAPLALTTKTKMQS
                     KINENIAAASSTPFSSPVNLSTSGRRTPGNQTPVMPSASPILHSQGKEKAVSNNVNPVK
                     TQHHSHPAKSLVEQFRGTDSDIPSSKDSEDSNEDEEEDDEEEGEEDDEDDESDDSQSES
                     DSNSESDTEGSEEEDDDDKDQDESDSDTEGEKTSMKLNKTTSSVKSPSMSLTGHSTPRN
                     LHIAKAPGPAPAALCSESQSPAFLGTSSSTLTSSPHSGTSKRRRVTDERELRIPLEYGW
                     QRETRIRNFGGRLQGEVAYYAPCGKKLRQYPEVIKYLSRNGIMDISRDNFSFSAKIRVG
                     DFYEARDGPQGMQWCLLKEEDVIPRIRAMEGRRGRPPNPDRQRAREESRMRRRKGRPPN
                     VGNAEFLDNADAKLLRKLQAQEIARQAAQIKLLRKLQKQEQARVAKEAKKQQAIMAAEE
                     KRKQKEQIKIMKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRIKE
                     KEMRRQQAVLLKHQERERRRQHMMLMKAMEARKKAEEKERLKQEKRDEKRLNKERKLEQ
                     RRLELEMAKELKKPNEDMCLADQKPLPELPRIP"
     misc_feature    783..788
                     /label=SDS
     misc_feature    1839..1844
                     /label=SDS
     CDS             2696..2710
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     intron          3993..4033
                     /label=Splicing Donor
     misc_feature    4037..4070
                     /label=lox2272-JT1571
     repeat_region   4115..4255
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4115..4244
                     /label=AAV2 ITR
     rep_origin      4330..4785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5067..5171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5172..6032
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6203..6791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggagtctgga gaacggttac catcctcagc
      781 agcctcctct actacaccaa cttcatcttc gacaccttct gtggcttcag tagtttcaaa
      841 aggtggcctt tccactggag ttgcttcact tagctctaca atcaacccat gtggacattt
      901 attcagaaca gctggggatc aaccgtttaa cctgtccaca gtgtcgagtg ccttcccaat
      961 ggtcagccac ccagtctttg gtctacattc agccagctca gggcattcag aatttggtgg
     1021 tttggggaca cttggtacac ccacagcctt agccgcacat ccccaactag catcttttcc
     1081 aggtgcagaa tggtggcgaa caactgatgc tcatactcgt acaggagcaa ccttctttcc
     1141 accattactg ggaattccac cactatttgc tcccccagcc cagaatcatg attcttcttc
     1201 attccattca aggacttcgg gaaaaagtaa tcgaaatggt cccgaaaaag gtgtaaatgg
     1261 gtcaataaat ggaagtaata catcatctgt aattggtatc aacacatctg tactatccac
     1321 tactgcttca agttccatgg gacaaactaa aagtacaagc tcaggtggag gaaatcgaaa
     1381 atgtaatcag gaacaaagca aaaaccagcc tttggatgct agagttgaca aaatcaaaga
     1441 taagaaacca aggaagaaag caatggaaag ttctagcaac agtgatagtg attcatgcac
     1501 atcatcagac acctcaagtg aaggcattag tagcagtgat tcagatgatc tagaagaaga
     1561 tgaagaagaa gaagatcaaa gtattgaaga aagtgaagat gatgattctg attcagagag
     1621 tgaagcacaa cataaaagta acaaccaggt gctattacat ggtatttcag acccaaaagc
     1681 agatggacag aaagcaactg aaaaagccca ggaaaaaaga atacaccagc cattacctct
     1741 tgcgtctgaa tcccagactc actcattcca atcccagcag aagcagcctc aggttttgtc
     1801 acagcagctt ccatttattt tccaaagctc tcaggcaaag gaggaatctg tgaacaaaca
     1861 caccagtgta atacagtcta cgggattggt gtccaatgtg aaacctttat ctttggtaaa
     1921 tcaagccaaa aaggaaactt acatgaaact catagttcct tctcctgatg ttcttaaagc
     1981 agggaataaa aatacctctg aagaatctag ttcattgacc agtgaattga gatccaaacg
     2041 ggaacaatat aaacaggcat tcccatcaca gttaaagaaa caagagtcat cgaagagcct
     2101 gaagaaggtt attgcagctt tgtcaaatcc aaaagcaacc tctagttcac cagcacatcc
     2161 aaaacaaaca ttagaaaaca accacccaaa tccattcttg acaaatgcac ttttaggtaa
     2221 tcaccaacca aatggagtta ttcaaagtgt cattcaagaa gctcctctag cacttactac
     2281 caaaactaaa atgcagagca agattaatga aaacattgct gctgcaagta gcaccccttt
     2341 ttcctcacct gtaaatctga gtacaagtgg gagaagaacc cctggcaatc agacacctgt
     2401 aatgccctct gcctctccca tcctgcatag tcaagggaag gaaaaagcag ttagcaataa
     2461 tgtaaaccca gtaaaaacac agcatcactc ccatcctgca aaatctttag tggaacaatt
     2521 cagaggaaca gattcagaca ttcccagtag taaagattct gaagattcaa atgaggatga
     2581 agaggaagat gatgaagaag aaggtgagga agatgatgaa gatgatgaat ctgatgacag
     2641 ccaatcagaa tcagatagta attcagaatc agatacagaa ggatcagaag aagaagatga
     2701 tgatgataaa gaccaagatg aatcagatag tgatactgaa ggagagaaaa cttcaatgaa
     2761 actgaataaa acaacttcct ctgtcaaaag cccttccatg agtctcacag gtcactcaac
     2821 acctcgtaac ctccacatag caaaagcccc aggccctgct cctgctgcct tatgttctga
     2881 atcccagtca cctgcttttc ttggtacatc ttcttccaca cttacttcaa gcccacactc
     2941 tggcacttcc aaaagaagaa gagtaacaga tgaacgtgaa ctgcgtattc cattggaata
     3001 tggctggcag agagagacaa gaataagaaa ctttggaggg cgccttcaag gagaagtagc
     3061 atattatgct ccatgtggaa agaaacttag gcagtaccct gaagtaataa agtatctcag
     3121 cagaaatgga ataatggata tctcaaggga caatttcagc ttcagtgcaa aaataagagt
     3181 gggtgacttc tatgaagcca gagatggacc gcagggaatg cagtggtgtc ttttgaaaga
     3241 agaggatgtc attcctcgta tcagggcaat ggaaggtcgt agaggaagac caccaaatcc
     3301 agatagacaa cgagcaagag aggaatccag gatgagacgt cggaaaggtc gacctccaaa
     3361 tgttggcaat gctgaattcc tagataacgc agatgcaaag ttgctaagaa aactgcaagc
     3421 tcaagaaata gccaggcaag cagcacaaat aaagcttttg agaaaacttc aaaagcagga
     3481 acaggctcgg gttgctaaag aagccaaaaa acaacaagca ataatggctg ctgaggagaa
     3541 gcggaagcaa aaagaacaga taaagattat gaaacagcag gaaaaaatta agagaataca
     3601 gcaaatcaga atggaaaaag aacttcgagc tcagcaaatt ctagaggcta aaaagaaaaa
     3661 gaaggaagaa gcggcaaatg ccaaattatt ggaggccgag aaacgaataa aggaaaaaga
     3721 aatgagaaga caacaagctg ttcttctgaa acatcaggaa cgagagcgaa ggcgacaaca
     3781 catgatgctt atgaaagcta tggaagctcg taaaaaagca gaagaaaaag agcggttgaa
     3841 acaagaaaaa cgtgatgaga aaagattaaa taaagagcgt aaactagagc agcgaagatt
     3901 agaattagaa atggcaaagg aactaaagaa gcctaatgaa gacatgtgct tagcagacca
     3961 aaagcctttg ccagagttgc ctcgtattcc aggtaagtat caaggttaca agacaggttt
     4021 aaggagacca ataagctatt attcgtatag gatactttat acgaagttat taagttatgg
     4081 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4141 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4201 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4261 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4321 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4381 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     4441 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4501 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4561 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4621 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     4681 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     4741 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4801 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4861 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4921 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4981 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5041 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5101 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5281 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5341 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5401 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5461 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5521 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5581 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5641 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5701 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5761 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5821 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5881 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5941 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6001 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6061 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6121 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6841 ttgctcacat gt
//