pcambia1303

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pCAMBIA1303
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: 35S
载体大小: 12362 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13 Forward:TGTAAAACGACGGCCAGT
载体标签: mGFP5、GUS
载体抗性: 卡那霉素
筛选标记: 潮霉素
克隆菌株: HB101等菌株
宿主细胞(系): 农杆菌/植物细胞
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA1303载体图谱



pCAMBIA1303 多克隆位点

载体简介

载体序列

LOCUS       AF234299               12361 bp    DNA     circular SYN 24-APR-2000
DEFINITION  Binary vector pCAMBIA-1303, complete sequence.
ACCESSION   AF234299
VERSION     AF234299.1  GI:7638078
KEYWORDS    .
SOURCE      Binary vector pCAMBIA-1303
  ORGANISM  Binary vector pCAMBIA-1303
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
            for plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
   PUBMED   7919218
REFERENCE   2  (bases 1 to 12361)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     A comprehensive set of modular vectors for advanced manipulations
            and efficient transformation of plants
  JOURNAL   Unpublished
  REMARK    Full description of constructs
REFERENCE   3  (bases 1 to 12361)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
            GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Binary vector pCAMBIA-1303"
                     /mol_type="other DNA"
                     /db_xref="taxon:118391"
     CDS             2..2569
                     /note="gusA (N358Q) - mgfp5* gene fusion; His6"
                     /codon_start=1
                     /product="GUSA (N358Q)/MGFP5 fusion, hexaHis tagged"
                     /protein_id="AAF65347.1"
                     /db_xref="GI:7638079"
                     /translation="MVDLTSLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESAL
                     QESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKV
                     WVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVS
                     VELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
                     RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTS
                     HYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQA
                     HLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVN
                     VMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIIT
                     EYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGIL
                     RVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASKGEELFTGVVPILV
                     ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRY
                     PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
                     DGNILGHKLEYNYNSHNVYIMADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGD
                     GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKASHHHHHHV"
     misc_feature    1088..1090
                     /note="N358Q glycosylation mutation"
     misc_feature    2598..2850
                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
     misc_feature    2888..2913
                     /note="right border T-DNA repeat"
     misc_feature    complement(3954..4954)
                     /note="STA region from pVS1 plasmid"
     rep_origin      complement(5547..6547)
                     /note="pVS1-REP; replication origin from pVS1"
     misc_feature    complement(6957..7217)
                     /note="bom site from pBR322"
     rep_origin      complement(7357..7637)
                     /note="pBR322 origin of replication"
     CDS             complement(7928..8722)
                     /note="aadA (kanamycin resistance) gene amplified from
                     pIG121Hm"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAF65349.1"
                     /db_xref="GI:7638081"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9147..9172
                     /note="left border repeat from C58 T-DNA"
     misc_feature    9239..9447
                     /note="CaMV 3'UTR (polyA signal)"
     CDS             complement(9463..10488)
                     /note="hptII (hygromycin resistance) gene"
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /protein_id="AAF65350.1"
                     /db_xref="GI:7638082"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKK"
     promoter        complement(10524..11331)
                     /note="CaMV35S2; CaMV 35S promoter, duplicated"
     promoter        11452..11506
                     /note="PlacZ; lacZ promoter"
     CDS             11534..11767
                     /codon_start=1
                     /product="LacZ alpha fragment"
                     /protein_id="AAF65348.1"
                     /db_xref="GI:7638080"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    11576..11626
                     /note="pUC18 MCS; polylinker"
     promoter        11818..12355
                     /note="CaMV35S; 35S promoter from CaMV"
ORIGIN      
        1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
       61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
      121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
      181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
      241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
      301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
      361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
      421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
      481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
      541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
      601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
      661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
      721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
      781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
      841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
      901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
      961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
     1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
     1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
     1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
     1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
     1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     1801 aaaaccgcag cagggaggca aacaagctag taaaggagaa gaacttttca ctggagttgt
     1861 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1921 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1981 actacctgtt ccgtggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc
     2041 aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata
     2101 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt
     2161 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga
     2221 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat
     2281 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga
     2341 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc
     2401 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     2461 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2521 catggatgaa ctatacaaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca
     2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
     2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
     7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
     8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
     8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
     8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
     8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
     9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
     9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
     9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg
     9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
     9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
    10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
    10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc
    10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
    10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac
    10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
    10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
    10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
    10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
    10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
    10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg
    10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
    10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
    10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
    10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
    10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
    10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
    11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
    11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
    11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
    11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
    11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
    11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
    11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
    11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
    12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
    12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
    12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
    12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
    12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
    12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
    12361 c
//

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